The invention discloses a method for detecting enantiomer in a vildagliptin midbody. The chromatographic conditions of the method are that a CHIRALPAK IC chromatographic column is adopted, the column temperature is 25-40 DEG C, the detection wavelength is 210nm, the mobile phase is a mixture of n-butyl alcohol, ethanol and diethylamine, and the flow velocity is 0.8-1.2mL/min. By adopting the method disclosed by the invention, quantitative analysis on the enantiomer (2R)-1-(2-chloracetyl)-2-pyrrolidine formonitrile in (2S)-1-(2-chloracetyl)-2-pyrrolidine formonitrile can be implemented, the enantiomer can be effectively separated and detected, peak patterns are in symmetry without trailing, aspects such as the separation degree, the specificity, the limit of quantitation, the detection limit, the linearity, the precision degree, the accuracy degree, the solution stability and the durability need to be specifically verified, various verification results meet requirements of related rules and guidance principles, and a good detection effect can be achieved.