27 results about "Metabolomic profiling" patented technology

The invention belongs to the technical field of compound analysis and identification, and discloses application of a metabonomics analysis technology to identification of lycium seed oil. According to the metabonomics analysis technology, the characteristic components of the lycium seed oil are identified through Log2 Fold Channel and VIP values, and the screening conditions for identifying the characteristic components of the lycium seed oil are | Log2 Fold Channel | > 1.5, and VIP < gt >; the method specifically comprises the steps of sample preparation, non-targeted metabonomics analysis, extraction and identification of characteristic peaks, similarity analysis, difference analysis and screening of differential markers. According to the method, a non-targeted metabonomics analysis method is adopted, various metabolic components in the lycium barbarum seed oil can be undiscriminately covered, the limitation that a traditional identification technology only aims at a few indexes is broken through, the unique characteristic components of the lycium barbarum seed oil can be accurately screened out, and the accuracy and specificity of an identification result are greatly improved.

The invention provides an iron-based nano material, a preparation method and application, the material comprises magnetic iron oxide nano particles and a functional modification layer on the surface, the magnetic iron oxide nano particles are Fe3O4, the particle size is 10-30 nm, and the magnetic iron oxide nano particles have specific oxygen vacancy concentration, 337 nm laser absorptivity and saturation magnetization; the functional modification layer is an amino-containing polymer layer, such as a polydopamine or cysteine-polyethyleneimine copolymer, and can reduce salt ion interference. The material solves the problems of low molecular weight region background interference, non-uniform crystallization, low ionization efficiency, poor salt tolerance and the like during detection of 100-400Da small molecular metabolites in trace samples such as tears and the like by optimizing magnetic responsiveness, photo-thermal conversion capability and salt resistance of an existing matrix; the method realizes high sensitivity and high repeatability (detection), and is suitable for metabonomics analysis of trace biological samples.

The invention relates to the technical field of biological detection, and discloses a metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method which comprises the following specific steps: S1, constructing a compound pollutant exposure system; s2, carrying out nematode culture and exposure treatment; s3, extracting in-vivo metabolites of the nematodes; s4, carrying out metabonomics analysis; s5, carrying out bioinformatics analysis; and S6, verifying the combined toxicity effect. By establishing the metabonomics-based caenorhabditis elegans compound pollutant exposure evaluation method, the limitation of a traditional single pollutant evaluation system is broken through, and systematic and high-sensitivity detection of multi-pollutant combined toxicity is realized; in addition, by constructing a standardized composite exposure system and an efficient metabolite extraction process and combining multi-dimensional mass spectrum data and bioinformatics modeling, a synergistic, antagonistic or additive effect mechanism among pollutants is deeply analyzed from the molecular level.

This invention relates to a method for compressing GC-HRMS fingerprint data, specifically for metabolomics analysis of plant, food essential oils, or biological samples. The method includes three steps: extraction of EICs, column isometry, and row isometry, which converts the data into an equal-row, equal-column matrix comparable to the original data, and then combines this with classical chemometric methods. This method overcomes the drawbacks of existing GC-HRMS fingerprints, such as their massive size and complexity, and is also applicable to LC-HRMS data. While the data compression method may sacrifice m / z accuracy, the peak intensities corresponding to each m / z range can be placed in their appropriate dictionary positions, ensuring accurate m / z readings in subsequent mass spectrometry. After row isometry, the retention time remains unchanged compared to the original data. Compared to existing ROI techniques, this data compression method offers significantly faster processing speeds.

OF THE INVENTION USE OF INHIBITORS OF THE N-ACETYLASPARTATE SYNTHETASE FOR THE TREATMENT OF ACUTE MYELOID LEUKEMIAS Acute Myeloid Leukemias (AML) are characterized by the proliferation of immature blood cells, leading to suppression of normal hematopoiesis. Despite existing treatments, resistance and relapse are common. A metabolomic analysis revealed increased N-Acetyl-Aspartate (NAA) levels in leukemic cells compared to normal HSCs, with high NAA levels linked to poor prognosis. The inventors studied the gene NAT8L, responsible for NAA synthesis, and found correlations with patient survival. Using CRISPR-cas9, the inventors targeted NAT8L in AML cell lines and observed that NAA depletion improved response to treatments and increased survival in vivo. Inhibiting NAA synthetase thus represents a therapeutic strategy to enhance chemotherapy efficacy and prevent relapse in AML patients.

The invention discloses an anti-aging drug screening method based on metabonomics. Firstly, a group of biomarkers, including L-valine, 4-hydroxyproline, L-serine, L-leucine, L-proline and melatonin, which are closely related to the caenorhabditis elegans aging process are identified in the invention. On the basis, the invention provides a drug screening method which comprises the following steps: performing metabonomics analysis on the nematodes subjected to drug administration treatment, detecting the content change of the biomarker group, and if the content of the biomarker group has obvious callback (up-regulation) compared with that of an old control group, judging that the drug has a potential anti-aging effect. The technical defects that a traditional nematode life experiment is long in period, tedious in operation, low in flux, high in subjectivity and the like are overcome, and a new anti-aging drug screening strategy which is rapid, stable and efficient and can preliminarily reveal drug effects is provided.

The invention relates to the technical field of metabonomics analysis, in particular to application of a metabolic marker in preparation of a head and neck squamous cell carcinoma diagnostic product, a kit, a screening method of the metabolic marker of the head and neck squamous cell carcinoma, a diagnostic model and a construction method and application of the diagnostic model. The metabolic marker comprises at least one of lactic acid, sphingosine, cadaverine, uridine diphosphate, allantoic acid, hydroxyproline, sphingosine-1-phosphoric acid, indole-3-acetaldehyde, pantothenic acid, fumaric acid, malic acid, prostaglandin or ornithine in red blood cells and / or plasma. The screened red blood cells and plasma metabolism markers can be used for predicting or diagnosing the head and neck squamous cell carcinoma respectively or independently, particularly, the red blood cell metabolism markers can provide more stable tumor microenvironment information, and the metabolism stability is high and is not easily influenced by diet and circadian rhythm.

The invention discloses a method for preventing and treating fusarium oxysporum by using trans-cinnamic acid and palmitic acid, and belongs to the technical field of biological prevention and treatment of plant diseases. The bacillus subtilis B579 and the fusarium oxysporum (FOC) are co-cultured to induce bacteriostatic metabolites, key bacteriostatic metabolites including trans-cinnamic acid (CA) and palmitic acid (PA) are screened out through metabonomics analysis, the optimal bacteriostatic concentration of the key bacteriostatic metabolites is determined to be 50, and the bacteriostatic effect is achieved by destroying the completeness of cell membranes of pathogenic bacteria and initiating membrane lipid peroxidation. The invention further provides a biological agent containing CA and PA and an application method, greenhouse pot experiment verifies that the prevention and control effects on cucumber fusarium wilt respectively reach 67.58% (CA) and 47.29% (PA), the environmental compatibility is excellent, and an efficient and safe prevention and control scheme is provided for sustainable development of the facility cucumber industry.

The invention discloses a method for enhancing the synthetic ability of yeast total protein and dipeptide tripeptide. Relates to the technical field of synthetic biology and metabolic engineering. According to the invention, the saccharomyces cerevisiae engineering strain with high protein synthesis capability and high endogenous dipeptide and tripeptide accumulation level is successfully constructed. The metabonomics analysis reveals the unique peptide fragment accumulation spectrum, especially the generation of a large amount of lysine-rich tripeptide at the C terminal, and proposes a potential mechanism of metabolic pathway'bottleneck 'caused by peptidase activity down-regulation in combination with proteome data. The recombinant strain has important application potential in the fields of single-cell protein, functional peptide products and the like.

A method for metabolomic profiling of a holobiont comprises the steps of subjecting a sample from a holobiont to chromatography-tandem mass spectrometry to acquire sets of experimental mass spectra, matching the sets of experimental mass spectra against library mass spectra of known metabolites to identify likely candidate metabolites in the holobiont sample, calculating predicted mass spectrometry fragmentation patterns and / or predicted chromatographic retention times and / or predicted collision cross sections for likely candidate metabolites, matching the sets of experimental mass spectra against the predicted mass spectrometry fragmentation patterns and / or predicted chromatographic retention times and / or predicted collision cross sections, and using a similarity metric to quantify the relationship between the experimental mass spectra and the library spectra candidates, predicted mass spectra candidates, and predicted chromatographic retention times and / or collision cross sections to rank the candidates by their combined relationship to the experimental signals in order to identify metabolites present in the holobiont sample.

The application provides a screening method for a wheat seed storage-related gene, and belongs to the field of agricultural biotechnology.The screening method comprises the following steps: 1) subjecting seeds of a storage-tolerant variety N38 and a storage-intolerant variety N5 to artificial aging treatment, taking the seed embryos after germination; 2) subjecting the seed embryos to metabolomics analysis to obtain differential metabolites; 3) extracting total RNA of the seed embryos, performing transcriptome sequencing, and screening candidate differential expression genes; and 4) screening the candidate differential expression genes associated with the obtained differential metabolites to obtain final screening differential expression genes.The application subjects known storage-tolerant and storage-intolerant wheat varieties to artificial aging, performs metabolomics detection and transcriptome sequencing, screens differential metabolites and candidate differential expression genes, and further analyzes to obtain a wheat seed storage-related gene, thereby providing key gene resources and technical support for wheat storage-tolerant molecular breeding.

The invention discloses a space metabonomics analysis method and application thereof, relates to the technical field of metabonomics analysis, and solves the problem that an analysis result of an existing conventional space metabonomics analysis method is not accurate enough. To-be-analyzed biological tissue samples are taken and divided into groups; preparing all groups of samples into slices, adsorbing the slices on the same glass slide, carrying out mass spectrum imaging detection, and synchronously collecting metabolite spatial distribution signals and mass spectrum intensity data of all the samples; the method comprises the following steps: preprocessing collected original data, identifying the distribution condition of known exogenous components in a medicine in a tissue slice, and delimiting a region with high signal intensity as a region of interest; randomly selecting a plurality of pixel points in the region of interest of each sample; and integrating the mass spectrum data corresponding to the pixel points selected from all the parallel samples in the same group, and sequentially carrying out PCA, OPLS-DA, differential metabolite screening and KEGG pathway enrichment analysis. The method can be applied to research on the hepatotoxicity of the polygonum multiflorum and the prepared polygonum multiflorum.

The invention relates to an artificial assistance and cattle mating method. The artificial assistance and cattle mating method comprises the steps that genital tract microenvironment parameters of to-be-mated cattle are monitored in real time, and the microenvironment parameters at least comprise the pH value, the temperature, the oxygen content and the metabolite concentration; collecting multi-dimensional data of the physiological state of the cattle to be hybridized, and preprocessing the multi-dimensional data and the microenvironment parameters through an edge calculation unit; analyzing the embryonic development state based on metabonomics and evaluating the quality; a micro-fluidic technology is combined to realize accurate regulation and control of a microenvironment; a hierarchical time memory network model and a Bayesian network are applied to construct an early pregnancy state prediction model, and high-precision pregnancy result prediction within 7-10 days after hybridization is achieved; according to the artificial assistance and cattle mating method, through multi-dimensional monitoring and accurate regulation and control, the pregnancy success rate is remarkably increased, and the breeding management cost is reduced.

The invention discloses a method for screening a drought-tolerant alfalfa variety, and relates to the technical field of forage grass breeding, and the method comprises the following specific steps: uniformly treating seeds, planting and culturing, carrying out drought stress treatment in a four-leaf stage, collecting a leaf sample in a stress later stage, carrying out liquid nitrogen cryopreservation, and obtaining metabolite data by using a chromatography-mass spectrometry technology, a characteristic metabolite fingerprint spectrum is constructed, drought-resistant alfalfa varieties and materials are screened in combination with a cosine similarity algorithm, a complete technical path from seeds to variety and material identification and screening is formed, and efficient screening and breeding of the drought-resistant varieties are achieved. According to the method, interference is reduced through a standardized process, reliable data is provided in combination with metabonomics analysis, screening scientificity is improved by constructing a characteristic metabolite fingerprint spectrum and a cosine similarity algorithm, result stability is ensured through a preset threshold value and repeated verification, batch screening and variety improvement are supported, the operation threshold is reduced, drought-enduring varieties are accurately recognized, and the method is suitable for large-scale popularization and application. And the alfalfa variety improvement and industrial application are efficiently promoted.

This invention belongs to the field of modified protein technology, and provides a shiitake mushroom protein crude extract-β-glucan complex and its application. The shiitake mushroom variety includes Shenxiang 1513, and the origin of Shenxiang 1513 includes Hubei or Zhejiang. The β-glucan includes at least one of barley β-glucan, oat β-glucan, and yeast β-glucan. This invention found that, compared with shiitake mushroom protein crude extract alone, the shiitake mushroom protein crude extract-β-glucan complex significantly increased the production of short-chain fatty acids and promoted the proliferation of beneficial bacteria. Simultaneously, this invention, through microbiome and metabolomics analysis, identified Bacillus phylum as a key positive regulator and confirmed its association with the upregulation of metabolic pathways such as purines, lipids, bile acids, and amino acids. These findings indicate that the shiitake mushroom protein crude extract-β-glucan complex of this invention has superior prebiotic potential, providing a theoretical basis for the development of functional food ingredients.

The invention discloses a method for analyzing an anti-inflammatory mechanism of total flavonoids of abelmoschus manihot based on UHPLC-QTOF-MS (Ultra High Performance Liquid Chromatography-Quantitative Time of Flight Mass Spectrometry) cell metabonomics. The method comprises the following steps: optimizing extraction conditions of a metabonomics intracellular sample, inducing RAW 264.7 cells by utilizing lipopolysaccharide (LPS) to construct an inflammation model, extracting the intracellular sample by adopting an optimized extraction mode, and then separating and identifying intracellular metabolites of a control group, a model group and an administration group (medium, low and high TFA (total flavonoids of abelmoschus manihot) dosage groups) by utilizing a UHPLC-Q-TOF-MS (Ultra High Performance Liquid Chromatography-Quantitative Time of Flight Mass Spectrometry) method. Analyzing the obtained secondary mass spectrum data, screening out differential compounds in the three groups by utilizing multivariate variable statistical analysis, and identifying the differential compounds; metaboAnalyst 6.0 is used for carrying out enrichment analysis and metabolic pathway analysis on the screened differential metabolites, and the possible anti-inflammatory activity mechanism of the differential metabolites influencing the total flavonoids of the abelmoschus manihot is clarified. The invention provides an effective metabonomics analysis method for illuminating an anti-inflammatory activity mechanism of the total flavonoids of the abelmoschus manihot flowers.

Methods, apparatuses, and systems for determining a peritoneal transport status classification of a patient based on mass analyzing low volumes of peritoneal dialysis (PD) effluent evaluated using PD effluent fingerprints of known transport statuses to determine a peritoneal transport status classification of the patient are described. In one example, a method includes obtaining a volume of PD effluent of the dialysis patient, generating patient information via mass analysis of the volume of PD effluent, and determining patient profile information based on evaluating the patient information with a profile library, the patient profile information comprising a peritoneal transport status classification. The PD effluent fingerprints may include information of at least one biomarker including at least one of L-tryptophan, 2-methoxy-2-methylpopanoic acid, 2-hydroxy-3-methyl-butyric acid, L-glutamine, L-phenylalanine, L-leucine, L-serine, L-α-glycerophosphorylcholine, cis-cinnamic acid, L-tyrosine, uric acid, L-histidine, N1-acetylspermidine, L-isoleucine, 5′-methylthioadenosine, and 4-hydroxybenzoic acid.

The invention discloses a metabolome and transcriptome analysis method and application in the aspect of traditional Chinese medicine eye masks.The metabolome and transcriptome analysis method comprises the following steps that S1, experimental grouping is conducted, and experimental animals are divided into a control group, a model group and a treatment group according to the standard; s2, after the experiment is finished, collecting a sample for detection; s3, metabonomics analysis is carried out, and specific metabolites related to treatment are screened out; s4, carrying out transcriptomics analysis, and screening out specific genes related to treatment; and S5, according to an analysis result, optimizing drug components so as to regulate and control the metabolic pathway and the gene in a targeted manner. A research method is provided for a treatment scheme of the xerophthalmia, the traditional Chinese medicine eye pad is further applied and developed, the xerophthalmia is treated more accurately, immunity is adjusted, metabolism is balanced, safety is high, and no chemical medicine side effect exists.

The application provides a kind of assay method for screening specific liver injury components of Epimedium and Psoralea, comprising the steps of preparing mother liquor, preparing diluent, measuring after cell administration, animal experiment verification and metabolomics analysis.The application excavates the components of Epimedium and Psoralea medicinal materials, and finds that immune stress induced by TNF-alpha, immune promoting components in Epimedium and Psoralea and susceptible components of liver injury synergistically cause liver injury, which provides potential biological target for early warning, diagnosis and treatment of liver injury caused by compatibility of Epimedium and Psoralea, and ensures safe and reasonable use of clinical drugs.

The invention provides an application of a metabolic small molecule additive in promoting the development process of a bovine in-vitro embryo, and belongs to the technical field of embryo in-vitro production. In particular to application of a polypeptide substance N-acetylaspartic acid-glutamine (NAAG) in promoting blastocyst formation in a bovine in-vitro embryo culture process. The invention further relates to a preparation method of the polypeptide substance NAAG, and particularly relates to application of the polypeptide substance NAAG in promoting blastocyst formation in a bovine in-vitro embryo culture process. Metabonomics analysis and experimental results prove that by adding the NAAG into an in-vitro embryo culture system, the blastocyst formation rate of the bovine in-vitro fertilized embryo can be remarkably increased, and the development efficiency and development quality of the in-vitro embryo are improved. The invention provides a new technical means for optimizing a cattle in-vitro embryo culture system, improving the embryo breeding efficiency and promoting the rapid propagation of improved cattle breeds, and has important application value and popularization significance in the fields of animal husbandry genetic breeding and animal embryo engineering.

The invention relates to the technical field of agricultural product quality identification and origin traceability, in particular to a saffron crocus origin traceability method based on multi-omics analysis. The saffron crocus origin tracing method based on multi-omics analysis comprises the following steps: 1) metabonomics analysis; according to the method, metabonomics data and proteomics data are integrated, the high-resolution origin traceability model is constructed, accurate identification of saffron crocus in different origins is achieved, and technical support is provided for saffron crocus quality control and market supervision.

The invention discloses a biomarker combination for distinguishing kidney transplantation post-transplantation plant function delay duration and application of the biomarker combination. The combination consists of IL-15, CCL3, IL-7, CCL20 and IL-5 proteins, and can optionally comprise metabolites such as alpha-ketoglutaric acid and the like. Targeted proteomics and metabonomics analysis discovers that short-term DGF (SDGF) is characterized by acute inflammation and innate immune activation, and long-term DGF (LDGF) shows chronic immunoregulation accompanied by amino acid and energy metabolism disorder. Based on the combination, the invention further provides a corresponding in-vitro detection kit, a non-diagnostic distinguishing method and a data processing device. The early-stage accurate risk stratification of the DGF patient can be realized, a key basis is provided for clinical intervention decision, and the method has important clinical application value.

This invention discloses a miR3980b Its application in promoting flavonoid synthesis in rice grains belongs to the field of genetic engineering technology. This invention is the first to discover and verify this technology. miR3980b It is a negative regulator of flavonoid biosynthesis in rice. Through positive (overexpression) and negative (STTM silencing) genetic strategies, combined with physiological and biochemical assays and metabolomics analysis, it was confirmed that... miR3980b Negative regulatory function in flavonoid accumulation in rice grains. Through silencing... miR3980b , miR3980b The expression level was significantly reduced, while the total flavonoid and polyphenol content was significantly increased, further confirming... miR3980b miR3980b Negative regulation of flavonoid biosynthesis. This invention lays the theoretical foundation for cultivating rice varieties with increased flavonoid content.

Methods, apparatuses, and systems for determining a peritoneal transport status classification of a patient based on mass analyzing low volumes of peritoneal dialysis (PD) effluent evaluated using PD effluent fingerprints of known transport statuses to determine a peritoneal transport status classification of the patient are described. In one example, a method includes obtaining a volume of PD effluent of the dialysis patient, generating patient information via mass analysis of the volume of PD effluent, and determining patient profile information based on evaluating the patient information with a profile library, the patient profile information comprising a peritoneal transport status classification. The PD effluent fingerprints may include information of at least one biomarker including at least one of L-tryptophan, 2-methoxy-2-methylpopanoic acid, 2-hydroxy-3-methyl-butyric acid, L-glutamine, L-phenylalanine, L-leucine, L-serine, L-α-glycerophosphorylcholine, cis-cinnamic acid, L-tyrosine, uric acid, L-histidine, N1-acetylspermidine, L-isoleucine, 5'-methylthioadenosine, and 4-hydroxybenzoic acid.

The application discloses application of an ELOVL5 gene as a biomarker and a therapeutic target of esophagogastric junction adenocarcinoma, and relates to the technical field of biological medicine.The application is proved by clinical sample detection that ELOVL5 is highly expressed in esophagogastric junction adenocarcinoma tissue, and is significantly different from normal tissue adjacent to the cancer, and the area under the diagnostic curve shows excellent diagnostic efficiency, providing a high-specificity biomarker for early screening and differential diagnosis of the disease.Metabolomics analysis shows that the fatty acid elongation process mediated by ELOVL5 is abnormally up-regulated in the patient, revealing the molecular mechanism of its participation in tumorigenesis.Cell experiments further confirm that the esophagogastric junction adenocarcinoma cell proliferation can be effectively inhibited by knocking down the expression of ELOVL5, and the feasibility of the ELOVL5 as a therapeutic target is clear.The application provides a new strategy for the diagnosis, efficacy evaluation and targeted treatment of esophagogastric junction adenocarcinoma, and has important scientific significance and clinical transformation value.