Culture solution capable of improving content of resveratrol in polygonum cuspidatum callus and culture method

A callus and resveratrol technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as inability to meet market demand, achieve the effects of increasing yield, simple operation, and prolonging the linear growth period

Active Publication Date: 2016-05-18
PROYA COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In existing reports, resveratrol accounted for 0.01-0.03% of the dry weight of callus cells after treatment with a specific elicitor, which still cannot meet the market demand. content has become an urgent problem to be solved

Method used

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  • Culture solution capable of improving content of resveratrol in polygonum cuspidatum callus and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A culture method that can increase the resveratrol content in Polygonum cuspidatum callus, adopts the following steps:

[0015] A. Selection and pretreatment of explant materials: The stems of Polygonum cuspidatum with a seedling age of about one week are selected as explants. First cut the stems of Polygonum cuspidatum into 5~10cm stems, rinse with running water for 2~3h, place the stems on a sterilized ultra-clean workbench, soak in sterile water for 10 minutes, and then soak in 75% ethanol-water solution for disinfection 30s, rinse with sterile water for 2~3 times, then sterilize with 10% NaClO solution for 5min, and finally soak the material 3 times with sterile water for 10min, 5min, 3min, and put it on sterile filter paper to dry; Cut off the old cut surface at both ends of the stem, and cut into small sections about 0.5×0.5cm for use;

[0016] B. Induction of Polygonum cuspidatum callus: Put the sterilized explants in step A into solid medium, the medium composition ...

Embodiment 2

[0020] A culture method that can increase the resveratrol content in Polygonum cuspidatum callus, adopts the following steps:

[0021] A. Selection and pretreatment of explant materials: The stems of Polygonum cuspidatum with a seedling age of about one week are selected as explants. First cut the stems of Polygonum cuspidatum into 5~10cm stems, rinse with running water for 2~3h, place the stems on a sterilized ultra-clean workbench, soak in sterile water for 10 minutes, and then soak in 75% ethanol-water solution for disinfection 30s, rinse with sterile water for 2~3 times, then sterilize with 10% NaClO solution for 5min, and finally soak the material 3 times with sterile water for 10min, 5min, 3min, and put it on sterile filter paper to dry; Cut off the old cut surface at both ends of the stem, and cut into small sections about 0.5×0.5cm for use;

[0022] B. Induction of Polygonum cuspidatum callus: Put the sterilized explants in step A into solid medium, the medium composition ...

Embodiment 3

[0026] A culture method that can increase the resveratrol content in Polygonum cuspidatum callus, adopts the following steps:

[0027] A. Selection and pretreatment of explant materials:

[0028] The stem segments of Polygonum cuspidatum with a seedling age of about one week are selected as explants. First cut the stems of Polygonum cuspidatum into 5~10cm stems, rinse with running water for 2~3h, place the stems on a sterilized ultra-clean workbench, soak in sterile water for 10 minutes, and then soak in 75% ethanol-water solution for disinfection 30s, rinse with sterile water for 2~3 times, then sterilize with 10% NaClO solution for 5min, and finally soak the material 3 times with sterile water for 10min, 5min, 3min, and put it on sterile filter paper to dry; Cut off the old cut surface at both ends of the stem, and cut into small sections about 0.5×0.5cm for use;

[0029] B. Induction of Polygonum cuspidatum callus: Put the sterilized explants in step A into solid medium, the med...

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Abstract

The invention relates to a culture solution capable of improving the content of resveratrol in polygonum cuspidatum callus and a culture method. The culture solution is composed of an MS basic culture medium, 6-benaylaminopurine, 6-glycosylaminopurine, naphthylacetic acid, spermine, spermidine and sucrose, wherein the concentration of the 6-benaylaminopurine is 0.5mg / L-1.8mg / L, the concentration of the 6-glycosylaminopurine is 0.3mg / L-1.0mg / L, the concentration of the naphthylacetic acid is 0.15mg / L-0.45mg / L, the concentration of the spermine is 1.0mg / L-4.5mg / L, the concentration of the spermidine is 0.5mg / L-1.25mg / L and the concentration of the sucrose is 20g / L-50g / L; and the pH value of the culture solution is 5-6. When the polygonum cuspidatum callus is cultured by using the culture solution provided by the invention, the content of the resveratrol can reach 0.12%-0.18% of the dry cell weight; and the culture solution has the beneficial effect of improving the content of the resveratrol in the polygonum cuspidatum callus.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to a culture solution and a culture method that can increase the resveratrol content in Polygonum cuspidatum callus. technical background [0002] Resveratrol (Res for short) is a non-flavonoid polyphenol compound containing stilbene structure. Its chemical name is 3,4,5-trihydroxystilbene (also known as stilbene triphenol). It is widely present in Polygonum cuspidatum, peanuts, grapes, Lilu and other natural plants or fruits have been found in 72 plants of 21 families and 31 genera. Among them, the rhizome of Polygonum cuspidatum is the most abundant. [0003] Resveratrol has many functions such as anti-cancer, anti-tumor, anti-cardiovascular disease, anti-inflammatory, and anti-oxidation. It is widely used in medicine and food and health industries. In the cosmetics industry, resveratrol plays its role in capturing free radicals, antioxidant and inhibiting tyrosinase activity. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 黄洁芳王丽娜毕永贤殷海琴陈斌蒋丽刚
Owner PROYA COSMETICS
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