Method of extracting lagedium sibiricum type bitter principle from chicories
A technology of lettuce and bitters, which is applied in the field of separation, can solve problems such as the complexity of the separation process, and achieve the effect that the separation method is simple and feasible
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Embodiment 1
[0022] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:
[0023] Step 1: After pulverizing the chicory root, use water with a pH of 6.6 and a temperature of 75°C for ultrasonic-assisted extraction for 35 minutes. The mass ratio of material to water is 1:19. The extract is filtered and concentrated under reduced pressure to obtain the total extract;
[0024] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;
[0025] Step 3: After dissolving the ethyl acetate extract obtained in Step 2 with ethyl acetate, 120 mesh silica gel column chromatography gradient elution separation, the mobile phase A is ethyl acetate, the mobile phase B is formic acid, The mobile phase C is n-butanol, and the elution conditions are: 0-4min, 45% A, 50% B, 5...
Embodiment 2
[0029] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:
[0030] Step 1: After pulverizing the chicory root, use water with a pH of 6.8 and a temperature of 80°C for ultrasonic-assisted extraction for 40 minutes. The mass ratio of material to water is 1:20. The extract is filtered and concentrated under reduced pressure to obtain a total extract;
[0031] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;
[0032] Step 3: After dissolving the ethyl acetate extract obtained in step 2 with ethyl acetate, it is separated by 130 mesh silica gel column chromatography gradient elution. In the gradient elution, the mobile phase A is ethyl acetate and the mobile phase B is formic acid. The mobile phase C is n-butanol, and the elution condi...
Embodiment 3
[0036] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:
[0037] Step 1: After pulverizing the chicory root, ultrasonically assisted extraction with water with a pH of 7.0 and a temperature of 85°C for 45 minutes, the extract is filtered and concentrated under reduced pressure to obtain a total extract;
[0038] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;
[0039] Step 3: After dissolving the ethyl acetate extract obtained in step 2 with ethyl acetate, it is separated by 140 mesh silica gel column chromatography gradient elution. In the gradient elution, the mobile phase A is ethyl acetate and the mobile phase B is formic acid. The mobile phase C is n-butanol, and the elution conditions are: 0-4 min, 45% A, 50% B, 5% C; 4-10 ...
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