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Method of extracting lagedium sibiricum type bitter principle from chicories

A technology of lettuce and bitters, which is applied in the field of separation, can solve problems such as the complexity of the separation process, and achieve the effect that the separation method is simple and feasible

Active Publication Date: 2017-10-20
新疆正生营养研究院(有限公司)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The Chinese invention patent with the authorized announcement number of CN102351824B provides a preparation method of olectuca and lentopicrin. In the above method, high-speed counter-current chromatography is used to separate the celtuce and celtuce, but it involves five kinds of The configuration and separation operation of the mixed solvent of the components, the overall separation process is slightly complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:

[0023] Step 1: After pulverizing the chicory root, use water with a pH of 6.6 and a temperature of 75°C for ultrasonic-assisted extraction for 35 minutes. The mass ratio of material to water is 1:19. The extract is filtered and concentrated under reduced pressure to obtain the total extract;

[0024] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;

[0025] Step 3: After dissolving the ethyl acetate extract obtained in Step 2 with ethyl acetate, 120 mesh silica gel column chromatography gradient elution separation, the mobile phase A is ethyl acetate, the mobile phase B is formic acid, The mobile phase C is n-butanol, and the elution conditions are: 0-4min, 45% A, 50% B, 5...

Embodiment 2

[0029] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:

[0030] Step 1: After pulverizing the chicory root, use water with a pH of 6.8 and a temperature of 80°C for ultrasonic-assisted extraction for 40 minutes. The mass ratio of material to water is 1:20. The extract is filtered and concentrated under reduced pressure to obtain a total extract;

[0031] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;

[0032] Step 3: After dissolving the ethyl acetate extract obtained in step 2 with ethyl acetate, it is separated by 130 mesh silica gel column chromatography gradient elution. In the gradient elution, the mobile phase A is ethyl acetate and the mobile phase B is formic acid. The mobile phase C is n-butanol, and the elution condi...

Embodiment 3

[0036] A method for extracting bitterin from chicory, having such characteristics, includes the following steps:

[0037] Step 1: After pulverizing the chicory root, ultrasonically assisted extraction with water with a pH of 7.0 and a temperature of 85°C for 45 minutes, the extract is filtered and concentrated under reduced pressure to obtain a total extract;

[0038] Step 2: Disperse the total extract in water, extract the aqueous phase with petroleum ether and ethyl acetate in sequence, and obtain petroleum ether extract and ethyl acetate extract from the organic phase after distillation under reduced pressure;

[0039] Step 3: After dissolving the ethyl acetate extract obtained in step 2 with ethyl acetate, it is separated by 140 mesh silica gel column chromatography gradient elution. In the gradient elution, the mobile phase A is ethyl acetate and the mobile phase B is formic acid. The mobile phase C is n-butanol, and the elution conditions are: 0-4 min, 45% A, 50% B, 5% C; 4-10 ...

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PUM

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Abstract

The invention discloses a method of extracting lagedium sibiricum type bitter principle from chicories. The method comprises the following steps: crushing chicory roots, performing extracting with water; performing filtering, and performing reduced pressure concentration to obtain a total extract; dispersing the total extract into water; performing extraction with ethyl acetate; performing reduced pressure distillation to obtain an ethyl acetate extract; and dissolving the ethyl acetate extract obtained in the second step and performing gradient elution separation, wherein a mobile phase A is ethyl acetate, a mobile phase B is formic acid, a mobile phase C is n-butyl alcohol and the elution condition comprises 0-4 min, 45% A, 50% B and 5% C; 4-10 min, 45%-40% A, 50%-55% B and 5% C; 10-14 min, 40%-30% A, 55% B and 5%-15% C; and 14-20 min, 30% A, 55% B and 15% C. The extracting method provided by the invention is convenient and feasible, and the purities of separated lactucin and lactucopicrin are both higher than 98.0%.

Description

Technical field [0001] The present invention relates to a separation method, in particular to a method for extracting bittersweet from chicory. Background technique [0002] Chicory is also known as European chicory or French cocory. It is called "bitter cabbage" in Japan. It is a plant of the genus Compositae with the same medicine and food. Studies have shown that chicory contains bitter substances that are not found in general vegetables, such as horse chestnut cortex, horse chestnut cortex glycosides, lyctophyllin and lyctobacter, etc. These bitter substances have antibacterial properties, improve digestion, clear liver and gallbladder, and relieve Wine and other effects. [0003] At present, the research on the chemical constituents of plants of the genus Cichorium mainly focuses on sesquiterpenoids. The sesquiterpenes in chicory as long as there are Lactucanum and Lactucanum, the molecular formula of Lactucanum is C 15 H 16 O 5 ,The molecular formula of Lactucae is C 23 H 22...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/93
CPCC07D307/93
Inventor 林峰高敬铭许伟王晓雯
Owner 新疆正生营养研究院(有限公司)
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