Method for separating and extracting lycopene from fermentation liquor

A technology of lycopene and fermented liquid, applied in the direction of extraction and purification/separation, crystallization purification/separation, chemical instruments and methods, etc., can solve the problems of long chemical synthesis process route, food safety impact, toxic substance residue, etc., to achieve The effect of important application value, low cost and less impurities

Active Publication Date: 2021-03-12
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Lycopene can be artificially synthesized through the Wittig olefination reaction, but the chemical synthesis method has a long process route, relatively harsh reaction conditions, and low yield. At the same time, the product contains a large amount of toxic residues, which has a great impact on food safety.

Method used

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  • Method for separating and extracting lycopene from fermentation liquor
  • Method for separating and extracting lycopene from fermentation liquor
  • Method for separating and extracting lycopene from fermentation liquor

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0037] Embodiment 1, the establishment of the method for separating and extracting lycopene from fermented liquid

[0038] 1. Experimental exploration of separation and extraction of lycopene from fermentation broth

[0039] The experiment was repeated three times to take the average value, and the steps for each repetition were as follows:

[0040]1. Take the recombinant Escherichia coli LYC029 fermentation broth, centrifuge, and collect the bacterial cells.

[0041] 2. Take a 500mL Erlenmeyer flask, add 100g of bacteria and 400mL of ethyl acetate, mix well, soak for 1 day, and collect the supernatant. Take 1 mL supernatant with a pipette, dilute 100 times with acetone, and then detect OD 474nm and OD 506nm .

[0042] 3. After completing step 2, add 400mL ethyl acetate to the Erlenmeyer flask, mix well, continue soaking for 1 day, and collect the supernatant. Take 1 mL supernatant with a pipette, dilute 100 times with acetone, and then detect OD 474nm and OD 506nm .

...

Embodiment 2

[0066] Example 2, Experiment 1 of Separating and Extracting Lycopene from Recombinant Escherichia coli LYC029 Fermentation Broth

[0067] 1. Take 10L of recombinant E. coli LYC029 fermentation broth (the concentration of lycopene in the recombinant E. coli LYC029 fermentation broth is 4g / L), centrifuge in a tubular centrifuge, and collect 3.3kg of thalline.

[0068] 2. After completing step 1, take 40g of bacteria, add 40mL of ethanol to soak for 1 hour, filter, filter with a sand core funnel, and collect the precipitate (about 10g).

[0069] 3. After completing step 2, wash the precipitate twice with ethanol, and collect the bacteria powder (about 10g).

[0070] The method of each washing is as follows: slowly add 10 mL of ethanol to the precipitate for rinsing, stir evenly and filter with suction.

[0071] 4. After completing step 3, transfer the bacterial powder to a 1L three-necked flask, add 200mL of ethyl acetate, extract 3 times at 60°C, combine the ethyl acetate phase...

Embodiment 3

[0079] Example 3, Experiment 2 of Separating and Extracting Lycopene from Recombinant Escherichia coli LYC029 Fermentation Broth

[0080] 1. Take 30L of recombinant E. coli LYC029 fermentation broth (the concentration of lycopene in the recombinant E. coli LYC029 fermentation broth is 4g / L), centrifuge in a tubular centrifuge, and collect 9.9kg of thallus.

[0081] 2. After completing step 1, take 320g of bacteria, add 320mL of ethanol to soak for 2 hours, filter, and filter with a sand core funnel to collect the precipitate (about 80g).

[0082] 3. After completing step 2, wash the precipitate twice with ethanol, and collect the bacteria powder (about 80g).

[0083] The method of each washing is: slowly add 80mL of ethanol to the precipitate for rinsing, stir evenly and filter with suction.

[0084] 4. After completing step 3, transfer the bacterial powder to a three-necked flask, add 1.6L ethyl acetate, extract twice at 60°C, combine the ethyl acetate phase to obtain an org...

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Abstract

The invention discloses a method for separating and extracting lycopene from fermentation liquor. The method comprises the following steps: taking fermentation liquor containing lycopene, and collecting thalli; adding ethanol into the thalli to soak for 1-3h, and collecting precipitates; washing the precipitate with ethanol twice or more, and collecting bacterial powder; extracting the bacterial powder for 2-4 times by using ethyl acetate at 50-60 DEG C, and combining ethyl acetate phases to obtain an organic phase; concentrating the organic phase; adding ethanol, and crystallizing at 0-4 DEGC to obtain the lycopene crystal. By adopting the method provided by the invention to separate and extract lycopene from the fermentation liquor, the purity can reach 98% or above, and the yield can reach 75% or above. The method is simple in separation and purification process, high in product purity, low in cost and suitable for continuous production, and meets industrial requirements. The invention has important application value.

Description

technical field [0001] The invention belongs to the technical field of biochemical separation, and in particular relates to a method for separating and extracting lycopene from fermentation broth. Background technique [0002] Lycopene is a long-chain unsaturated olefin, a kind of carotenoid. Its antioxidant capacity is 3.2 times that of β-carotene and 100 times that of vitamin E. It can efficiently quench singlet oxygen in the human body. And scavenging free radicals, thereby playing the role of anti-cancer, tumor suppressor and activation of immune cells and other functions, known as "plant gold". [0003] At present, lycopene is mainly extracted from plants. Natural plants have the highest lycopene content in tomato, but the content is only about 100mg / kg. Large-scale extraction of lycopene will cause a lot of waste of plant resources, and the plant components are complex. The purity of the extracted product is not high. Therefore, considering factors such as quality, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C7/00C07C7/10C07C7/14C07C11/21
CPCC07C7/00C07C7/10C07C7/14C07C11/21Y02E50/30
Inventor 付绍平徐超夏海容唐金磊张学礼
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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