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Application of Polygonum multiflorum extract

A technology of Polygonum multiflorum and extract, which is applied in the application field of Polygonum multiflorum extract to control strawberry gray mold, can solve the problems of rare reports on the inhibitory effect of plant pathogens, achieve safety and environmental protection for humans and animals, and the extraction method is simple and easy to promote and apply the effect

Inactive Publication Date: 2013-11-20
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results of previous studies have shown that Polygonum multiflorum extract has a certain inhibitory effect on animal pathogenic bacteria, but there are few reports on the inhibitory effect on plant pathogenic bacteria.

Method used

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  • Application of Polygonum multiflorum extract
  • Application of Polygonum multiflorum extract
  • Application of Polygonum multiflorum extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Get Radix Polygoni Multiflori Radix Radix Root tuber, wash and dry, and use a high-speed pulverizer to pulverize to 75 mesh fine powder with the rotating speed of 3000r / min, obtain Radix Polygoni Multiflori powder. Take Polygonum multiflorum powder 10g and put it into a triangular flask, extract the Polygonum multiflorum powder with sterile water, add sterile water 3 times, add 30mL each time, put the triangular flask on a shaker at 25°C and shake at 100r / min 60h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, and evaporate and concentrate at 110r / min to a paste under the conditions of 0.08MPa air pressure, water bath temperature of 45°C and condensation temperature of 5°C to obtain Red-yellow Polygonum multiflorum extract.

Embodiment 2

[0022] Wash the Radix Polygoni Multiflori, dry it in the sun, and pulverize it into a fine powder of 85 mesh with a high-speed pulverizer at a speed of 4000 r / min to obtain Polygonum Polygoni Multiflori powder. Take Polygonum multiflorum powder 10g and put it into a triangular flask, extract the Polygonum multiflorum powder with sterile water, add sterile water 3 times, add 40mL each time, put the triangular flask on a shaker at 27°C and shake at 115r / min 48h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, and evaporate and concentrate at 120r / min to a paste under the conditions of 0.09MPa air pressure, water bath temperature of 50°C and condensation temperature of 7°C to obtain Red-yellow Polygonum multiflorum extract.

Embodiment 3

[0024] Wash the Radix Polygoni Multiflori, dry it in the sun, and pulverize it into a fine powder of 95 mesh with a high-speed pulverizer at a speed of 5000 r / min to obtain the Radix Polygonum multiflorum powder. Take Polygonum multiflorum powder 10g and put it into a triangular flask, extract the Polygonum multiflorum powder with sterile water, add sterile water 3 times, add 50mL each time, put the triangular flask on a shaker at 29°C and shake at 130r / min 36h, filter, collect the filtrate, combine the filtrate for 3 times, put the filtrate into a rotary concentration evaporator, and evaporate and concentrate at 130r / min to a paste under the conditions of 0.1MPa air pressure, water bath temperature of 55°C and condensation temperature of 9°C to obtain Red-yellow Polygonum multiflorum extract.

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Abstract

The invention relates to an application of a Polygonum multiflorum extract in controlling grey mould fruit rot of strawberry. The extract is Polygonum multiflorum earthnut which is extracted by sterile water. According to the invention, Polygonum multiflorum is taken as a plant source extract resource to extract antibacterial substances, and the extract has the inhibiting effect and a certain in-vitro control efficiency to mould fruit rot of strawberry and can be further developed to novel plant source bactericide.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and mainly relates to the application of a Polygonum multiflorum extract, in particular to the application of the Polygonum multiflorum extract in controlling gray mold of strawberries. Background technique [0002] Strawberry gray mold caused by Botrytis cinerea Pers.et Tris. is one of the main diseases of strawberry. The safe production of ground strawberries poses a threat. Since the disease occurred, it has been reported all over the world and has become a restrictive obstacle to strawberry production. At present, the source of resistance to strawberry gray mold has not been found in production, and chemical agents are mainly used for prevention and control, the most important of which are the use of benzimidazole and dicarboximide fungicides. The continuous use of bacteriocarbamide, pyrimethanil, carbendazim, kresoxim-methyl, fludioxonil and other agents has resulted in the resistanc...

Claims

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Application Information

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IPC IPC(8): A01N65/30A01P3/00
Inventor 廖晓兰张亚黄璜刘双清王凌宇鱼欢
Owner HUNAN AGRICULTURAL UNIV
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