Method for preparing high purity chlorogenic acid

A chlorogenic acid and high-purity technology, which is applied in the field of preparing high-purity chlorogenic acid, can solve the problems of high cost and long cycle of column chromatography, and achieve the effects of saving production cost, facilitating water-soluble impurities, and shortening the production cycle

Active Publication Date: 2012-04-04
GUANGXI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

Because the market price of honeysuckle is relatively expensive, this paper chooses the relatively low price of Guangxi's superior resource mountain silver flower as the research object. The present invention relates to a simple, quick and high extraction method to prepare chlorogenic acid crystals, eliminating the need for columnar layers Due to the disadvantages of long analysis period and high cost, it is suitable for industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 500g of Japonica japonica, ultrasonically extract 3 times at 60°C, add 5000ml of 80% ethanol water for extraction for 1 hour each time, combine the three extractions and concentrate under reduced pressure to form an extract, which is dissolved in 500ml of distilled water , adjust the pH to be 5, continuously extract 6 times with 500ml ethyl acetate, combine the extracts for 6 times, add 50g ammonium sulfate in the aqueous solution after extraction, adjust the pH to 3, then use 500ml ethanol: ethyl acetate volume ratio is The mixed solvent extraction of 1:9 was extracted 4 times, combined the extracts of these 4 times, and back-extracted once with 500ml polyacrylamide aqueous solution with a mass percentage of 1%, and then the extract after back-extraction was passed through an activated carbon column, and the eluent was reduced Concentrate under pressure and dry in vacuo to obtain crude chlorogenic acid, dissolve the crude chlorogenic acid with 40ml ethyl acetate a...

Embodiment 2

[0030] Weigh 500g of Japonica japonica, ultrasonically extract 3 times at 60°C, add 5000ml of 80% ethanol water for extraction for 1 hour each time, combine the three extractions and concentrate under reduced pressure to form an extract, which is dissolved in 500ml of distilled water , adjust the pH to be 6, use 500ml ethyl acetate to continuously extract 5 times, combine the extracts for 5 times, add 50g ammonium sulfate in the aqueous solution after extraction, adjust the pH to 4, then use 500ml ethanol: ethyl acetate volume ratio is The mixed solvent extraction of 1:9 was extracted 5 times, and the extracts of these 5 times were combined, and the polyacrylamide aqueous solution 500ml with a mass percentage of 1% was used for back extraction once, and then the extract after back extraction was passed through an activated carbon column, and the eluent was reduced Concentrate under pressure and dry in vacuo to obtain crude chlorogenic acid, dissolve the crude chlorogenic acid w...

Embodiment 3

[0032] Weigh 500g of Japonica japonica, ultrasonically extract 3 times at 60°C, add 5000ml of 80% ethanol water for extraction for 1 hour each time, combine the three extractions and concentrate under reduced pressure to form an extract, which is dissolved in 500ml of distilled water , adjust the pH to be 5, continuously extract 5 times with 500ml ethyl acetate, combine the extracts for 5 times, add 50g ammonium sulfate in the aqueous solution after extraction, adjust the pH to 3, then use 500ml ethanol: ethyl acetate volume ratio is The mixed solvent extraction of 1:9 was extracted 5 times, and the extracts of these 5 times were combined, and the polyacrylamide aqueous solution 500ml with a mass percentage of 1% was used for back extraction once, and then the extract after back extraction was passed through an activated carbon column, and the eluent was reduced Concentrate under pressure and dry in vacuo to obtain crude chlorogenic acid, dissolve the crude chlorogenic acid wit...

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Abstract

The invention discloses a method for preparing high purity chlorogenic acid. The method comprises the following steps of: preparing a lonicera confusa extract from lonicera confuse serving as a raw material by using a mixed solvent of ethanol and water under the ultrasonic condition, dissolving the extract in water, selectively extracting chlorogenic acid from the aqueous solution, performing back-extraction on extract liquor by using an aqueous solution of polyacrylamide to remove partial high-polarity impurities, decoloring the extract liquor subjected to back-extraction by using active carbon, and recrystallizing to obtain chlorogenic acid crystals. The method is easy to impellent, the production period is short, the yield and purity of the product are high, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from plants. Specifically, it is a method for preparing high-purity chlorogenic acid. technical background [0002] Chlorogenic acid has a wide range of biological activities. Modern scientific research on the biological activity of chlorogenic acid has penetrated into many fields such as food, health care, medicine and daily chemical industry. Chlorogenic acid is an important biologically active substance, which has the functions of antibacterial, antiviral, increasing white blood cells, protecting liver and gallbladder, antitumor, lowering blood pressure, lowering blood fat, scavenging free radicals and stimulating the central nervous system. Although my country has natural resources such as honeysuckle, eucommia and sunflower seeds rich in chlorogenic acid, domestic research on the development and utilization of chlorogenic acid is still far behind developed countries. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/48
Inventor 韦万兴崔春雨周敏凌秀荣
Owner GUANGXI UNIV
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