Probiotic composition for inhibiting streptococcus mutans and application of probiotic composition

A technology of Streptococcus mutans and probiotics, applied in the field of probiotics, can solve problems such as dental fluorosis

Inactive Publication Date: 2021-08-20
ALAND BIOTECHNOLOGY RES TAIZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fluoride-containing toothpaste can enhance the anti-acid and calcium-dissolving ability of tooth enamel to a certain extent, but in the process of using it, we must pay attention to the excessive intake of fluoride. Excessive intake of children will lead to dental fluorosis.

Method used

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  • Probiotic composition for inhibiting streptococcus mutans and application of probiotic composition
  • Probiotic composition for inhibiting streptococcus mutans and application of probiotic composition
  • Probiotic composition for inhibiting streptococcus mutans and application of probiotic composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Screening and Identification of Strains

[0044] Selective medium (Bifidobacterium: TPY agar; Lactobacillus: modified MC agar) was used to isolate the strains from the intestines of healthy young people and children. gram, placed in a sterile collection tube, added 50ml of sterile water within 1 hour, and oscillated to make a homogenate. Serially dilute 10-fold concentration with sterile water to 10 -5 ;Take 0.1ml of samples with different dilutions and spread them on the two selective media, and incubate anaerobically at 37°C for 48 hours; take out the petri dish, pick single colonies with different morphological characteristics, and transfer them to liquid MRS broth medium, 37 After 24 hours of anaerobic culture at ℃, send it to the sequencing company to detect and identify the strains, and the identified strains can be frozen for preservation after three consecutive passages.

[0045] The single colony shape of Lactobacillus rhamnosus strain AI-11 is white to trans...

Embodiment 2

[0051] Safety Evaluation of Strains

[0052] The -20°C cryopreserved glycerol tube strain obtained in Example 1 was inoculated and resuscitated in a normal MRS broth medium, and subjected to three transfer activation cultures (anaerobic culture at 37°C for 24 h), and the activated culture solution was used for Antibiotic susceptibility testing.

[0053] Take 0.5mL of each activated bacterial strain, spread it evenly on the surface of MRS agar plate, and after drying, add gentamicin, vancomycin, kanamycin, clindamycin, streptomycin, ampicillin , penicillin, erythromycin, and chloramphenicol were pasted on the surface of the agar, and placed in a 37°C incubator for 24 hours to observe the growth of the strains on the agar surface. If an obvious transparent circle appears around the antibiotic paper, measure the diameter of the transparent circle with a ruler to determine whether the tested strain is drug-sensitive. At the same time, Staphylococcus aureus was selected as the qu...

Embodiment 3

[0062] The general Oxford cup method was used to determine the inhibitory effect of co-culture strains on caries-forming bacteria (Streptococcus mutans): 4 Oxford cups were evenly placed on the solid brain-heart infusion agar BHI plate for cultivating Streptococcus mutans, and each Oxford cup was added 200 μL of the supernatant of the corresponding probiotic bacteria liquid was placed in a 37°C incubator for overnight culture, and then the inhibition zone of the plate was measured. The results are shown in Table 3 and Figure 4 As shown, a single strain can inhibit Streptococcus mutans, but the co-cultivated strains Lactobacillus rhamnosus AI-11※Lactobacillus fermentum AI-25, Lactobacillus casei AI-12※Bifidobacterium animalis milk subspecies AI-01, Bifidobacterium infantis AI-20※Lactobacillus paracasei AI-62 has a better inhibitory effect on Streptococcus mutans than a single strain.

[0063] Table 3 co-cultivated bacterial strains to the inhibition zone effect of Streptococc...

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Abstract

The invention provides a probiotic composition for inhibiting streptococcus mutans and application of the probiotic composition, and relates to the technical field of probiotics. The probiotic composition comprises a bacterial solution formed by co-culturing lactobacillus rhamnosus and lactobacillus fermentum, a bacterial solution formed by co-culturing bifidobacterium animalissubsp.lactis and lactobacillus casei and/or a bacterial solution formed by co-culturing lactobacillus paracasei and bifidobacterium infants; and an inoculation culture medium for co-culturing comprises an MRS broth culture medium, and co-culturing is anaerobic culturing at 37 DEG C for 24 h. The probiotic composition shows a higher antibacterial effect on inhibiting dental caries generation bacteria (streptococcus mutans) than a single strain, and can be widely applied to probiotic health food through safety evaluation.

Description

technical field [0001] The invention belongs to the technical field of probiotics, and in particular relates to a probiotic composition for inhibiting streptococcus mutans and an application thereof. Background technique [0002] Probiotics refer to a class of active microorganisms that are beneficial to the human body, improve the micro-ecological balance of the host, and exert beneficial effects to produce certain health effects. With the continuous development and deepening of research on probiotics, the role of probiotics is no longer limited to the field of food, but gradually turned to the application of some common diseases including intestinal diseases, vaginitis, oral diseases, constipation, etc. prevention and treatment. It even played a huge role in some other diseases, such as the star strains of Lactobacillus casei, LGG and BB12, which played an important role in many diseases (obesity, type 2 diabetes, non-alcoholic fatty liver and cardiovascular and cerebrova...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135
CPCA23L33/135A23V2002/00A23V2400/143A23V2400/165A23V2400/125A23V2400/175A23V2400/515A23V2400/529A23V2200/312A23V2200/30
Inventor 李雪君张国栋赵宇徐成炜
Owner ALAND BIOTECHNOLOGY RES TAIZHOU CO LTD
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