Determination of microbial inhibition potency of macleaya cordata extract and minimal inhibitory concentration
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A technology of Boluohui extract and antibacterial concentration, applied in the field of breeding, can solve problems such as increased feed consumption, inability to completely replace antibiotics, and bacterial resistance
Inactive Publication Date: 2018-03-06
云南集睿科技有限公司
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Problems solved by technology
[0002] The use of antibiotics as feed additives can improve the health of piglets and increase feed utilization, but long-term large-scale, repeated use and abuse of antibiotics will lead to bacterial resistance and animal product residues, which seriously endanger human health
The long-term and extensive use of antibiotics has led to bacterial resistance and drug residues, which has aroused great concern in the society. The adverse effects of prohibiting the use of antibiotics on the pig breeding industry have gradually emerged. rate reduction
At present, there is no additive that can effectively improve the performance of pigs and improve feed utilization like antibiotics. If antibiotics are not used in pig feed, it will lead to increased feed consumption and decreased growth rate of pigs. Therefore, stop using antibiotics Feed additives must have new antibiotic substitutes to ensure the health and production efficiency of pigs. Currently commonly used enzyme preparations, trace element additives, and organic acids can improve pig production performance and feed utilization to varying degrees, but they cannot completely alternative to antibiotic use
[0003] At present, the current common method for piglet production is to directly apply special feed such as plasma protein powder in the piglet diet to increase the blood antibody IgG level of piglets, and the research and application of using special active substances to induce an increase in the IgG content in animals is relatively weak
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Embodiment 1
[0029] The mensuration of Boluohui extract to microorganism inhibitory effect comprises the following steps:
[0030] 1) Boluohui raw material extraction: take the dried aboveground part of Boluohui, grind it to 40 mesh thickness, add 80% ethanol with a pH of 5, and filter after refluxing for 2 hours, combine the filtrate and concentrate until the ethanol is recovered completely, add Equal volume of water, let it stand, get precipitate, remove the supernatant, and vacuum-dry the filter residue to obtain the orange powder boluohui extract;
[0031] 2) Preparation of the sample solution to be tested: take 20.0m of the Boluohui extract described in step 1) in a 250ml volumetric flask, add about 200ml of 0.1% phosphoric acid aqueous solution for ultrasonic treatment for 26min, cool to room temperature, and dilute with 0.1% phosphoric acid aqueous solution To the scale, shake well to obtain the test sample solution;
[0032] 3) Preparation of standard sample solution: Accurately w...
Embodiment 2
[0046] The mensuration of Boluohui extract to microorganism inhibitory effect comprises the following steps:
[0047] 1) Raw material extraction of Boluohui: take the dried aboveground part of Boluohui, crush it to a thickness of 50 meshes, add 80% ethanol with a pH of 5, filter after refluxing for 2 hours, combine the filtrates and concentrate until the ethanol is recovered completely, add Equal volume of water, let it stand, get precipitate, remove the supernatant, and vacuum-dry the filter residue to obtain the orange powder boluohui extract;
[0048] 2) Preparation of the sample solution to be tested: Take 20.0m of the Boluohui extract described in step 1) in a 250ml volumetric flask, add about 200ml of 0.1% phosphoric acid aqueous solution for ultrasonic treatment for 32min, cool to room temperature, and dilute with 0.1% phosphoric acid aqueous solution To the scale, shake well to obtain the test sample solution;
[0049] 3) Preparation of standard sample solution: Accur...
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Abstract
The invention discloses determination of microbial inhibition potency of macleaya cordata extract. The determination comprises the following steps: 1) extracting a macleaya cordata raw material; 2) preparing a to-be-determined sample solution; 3) preparing a standard sample solution; 4) preparing test culture medium; 5) determining antibacterial potency; and 6) respectively taking 0.1ml bacteria solution of escherichia coli, salmonellas, staphylococcus aureus and bacillus subtilis in a logarithm growth period in a control group, uniformly coating the surface of a common broth medium, taking sterilized filter paper pieces with the diameter of 6mm, immersing in 1.5mg/ml, 3.0mg/ml and 4.5mg/ml standard sample solution for 38-42 minutes, then taking out, putting into an incubator at the temperature of 37 DEG C, culturing for 24 hours, then observing, and recording the average value of diameters of inhibition zones of three filter paper pieces for each kind of bacteria. The determination disclosed by the invention has the advantages that a theoretical foundation is provided for applying macleaya cordata to replace antibiotics in piglet feed through study on the determination of the macleaya cordata extract; and by adopting a contrast test, content of macleaya cordata effective ingredient in the macleaya cordata extract can be determined more accurately, and use of the macleaya cordata is accurately controlled.
Description
technical field [0001] The invention belongs to the field of breeding, and in particular relates to the determination of the inhibitory effect of Boluohui extract on microorganisms and the minimum inhibitory concentration. Background technique [0002] The use of antibiotics as feed additives can improve the health of piglets and increase feed utilization, but long-term large-scale, repeated use and abuse of antibiotics will lead to bacterial resistance and animal product residues, which seriously endanger human health. The long-term and extensive use of antibiotics has led to bacterial resistance and drug residues, which has aroused great concern in the society. The adverse effects of prohibiting the use of antibiotics on the pig breeding industry have gradually emerged. rate decreased. At present, there is no additive that can effectively improve the performance of pigs and improve feed utilization like antibiotics. If antibiotics are not used in pig feed, it will lead to...
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