Method for testing sensitivity of pathogenic microorganisms to antibacterial drugs

Abstract

The invention relates to a method for testing sensitivity of pathogenic microorganisms to antibacterial drugs. The method comprises the following step of: contacting the antibacterial drugs with the pathogenic microorganisms in culture solution and is characterized in that the antibacterial drugs are dissolved in the culture solution in the form of sterile instant antibacterial drug films; the thickness of the sterile instant antibacterial drug films is 0.01-1mm; and the 1cm<2> films are completely dissolved in a 1ml broth culture medium in five minutes, preferably three minutes and more preferably 100 seconds. The invention also provides the sterile instant drug films used in the method and a preparation method and application thereof. By adopting the method, the operating efficiency, accuracy and economical efficiency of drug sensitivity test of the microorganisms can be improved, quantification can be achieved and cumbersome operation of the dilution method is omitted, and the method is more accurate and economical than E-test strips.

Description

technical field [0001] The invention relates to a method for testing microbial drug sensitivity, in particular to a method for testing the sensitivity of antibacterial drugs to pathogenic microorganisms, and belongs to the field of drugs. Background technique [0002] With the continuous emergence of new pathogenic bacteria, the control effect of antibacterial drugs is getting worse and worse. And various pathogenic bacteria have different sensitivities to different antibacterial drugs, and different strains of the same bacterium have different sensitivities to different antibacterial drugs. For a long time, the emergence of drug resistance of various pathogenic bacteria has caused various commonly used antibacterial drugs to lose their efficacy, and the sensitivity of drugs to bacteria cannot be well grasped. [0003] Despite the continuous emergence of new antibacterial drugs, the resistance of common infectious bacteria has also increased. In China, the detection rate o...

AI Technical Summary

Problems solved by technology

Of course, the instrument method is also inseparable from the manual preparation of the bacterial suspension in advance. The purity and concentration of the bacterial suspension will affect the results of the instrument. Since the instrument needs a matching antibacterial drug plate, its consumables are relatively expensive, and the antibacterial drug combination It is stipulated by the manufacturer and cannot be changed by the user at will

For some clinically relevant fastidious bacteria, as well as anaerobic bacteria, instrumental methods for some non-fermenting bacteria cannot be determined

For some bacteria with special drug resistance mechanism or low-level drug resistance, it cannot be detected by instrumental methods

Images