Preparation method of efficient active composite corruption decoloring inoculant

Abstract

A preparation method of an efficient active composite corruption decoloring inoculant comprises the following steps: carrying out corruption fermentation of a broth medium, dumping bacterial dyed wastewater, carrying out domestication culture, separating and screening three decolored bacteria, and finally determining Bacillus cereus, Clostridium perfringens and Shewanella putrefaciens according to the form characteristics and the culture characteristics of the three decolored bacteria and biochemical experiments of the three decolored bacteria; and respectively inoculating the three decolored bacteria preserved at a low temperature into an inclined plane nutritional agar culture medium, carrying out rejuvenation culture, carrying out composite culture of the three rejuvenated decolored bacterial in the broth culture medium to obtain a seed liquid, inoculating the seed liquid into a fermentation tank provided with the broth culture medium, and carrying out enlargement fermentation culture to prepare the efficient active composite corruption decoloring inoculant. The efficient active composite corruption decoloring inoculant has the advantages of simple preparation process, stable performances, use convenience, storage at normal temperature without inactivation, and realization of the bacterial dyed wastewater decoloring rate of 100%.

Description

technical field [0001] The invention provides a preparation method of a high-efficiency active compound putrefaction and decolorization bacterial agent, which belongs to the technical field of microbial preparations. Background technique [0002] At present, many countries and regions have developed a variety of high-efficiency microbial agents, which are made into commercial products for sale. The establishment of a microbial strain bank for environmental microbial preparations is the basis for promoting its industrial development to meet actual needs. The present invention is the application of microbial engineering in the field of environmental protection, especially the high-efficiency and active compound spoilage decolorization bacteria agent produced by fermentation technology is used to decolorize a large amount of bacterial dyeing wastewater produced every year in the microbiology laboratory. The main dyes in these wastewaters are basic dyes. They have a complex str...

AI Technical Summary

Problems solved by technology

[0003] The object of the present invention is to aim at the shortcomings of various pollutants in bacterial dyeing wastewater, complex structure, difficult to degrade, potential toxicity, and serious pollution to the environment caused by direct discharge, and to invent a method for preparing an efficient and active compound putrid decolorizing bacterial agent, comprising: