Preparation method of efficient active composite corruption decoloring inoculant

A decolorizing bacteria and high-efficiency technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of complex structure, serious environmental pollution, and difficult to degrade, and achieves simple preparation process and convenient use. , the effect of stable performance

Inactive Publication Date: 2013-04-24
LANZHOU JIAOTONG UNIV
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Problems solved by technology

[0003] The object of the present invention is to aim at the shortcomings of various pollutants in bacterial dyeing wastewater, complex structure, difficult to degrade, potential toxicity, and serious pollution to the environment caused by direct discharge, and to invent a method for preparing an efficient and active compound putrid decolorizing bacterial agent, comprising:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] An accidental discovery: Pour the prepared broth culture medium into an Erlenmeyer flask, put a cotton plug on the mouth of the bottle, boil it until it boils, and put it at room temperature (in summer). The milky white bacterial film was poured into the bacterial dyeing wastewater. After 48 hours, the color of the dye was completely removed, indicating that the natural corruption of the broth medium has a good decolorization function, so the following experimental process was established:

[0018] 1. Domestication and screening of three strains of decolorizing bacteria, including:

[0019] (1) Put the Erlenmeyer flask containing the broth culture medium, put a cotton plug on the mouth of the bottle, and put it in a constant temperature incubator at 35°C for rotten fermentation for 48 hours. The broth culture medium is turbid and a milky white bacterial film is formed on the surface. With a fragrant and rotten smell, the formula of the broth medium used is beef extract ...

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Abstract

A preparation method of an efficient active composite corruption decoloring inoculant comprises the following steps: carrying out corruption fermentation of a broth medium, dumping bacterial dyed wastewater, carrying out domestication culture, separating and screening three decolored bacteria, and finally determining Bacillus cereus, Clostridium perfringens and Shewanella putrefaciens according to the form characteristics and the culture characteristics of the three decolored bacteria and biochemical experiments of the three decolored bacteria; and respectively inoculating the three decolored bacteria preserved at a low temperature into an inclined plane nutritional agar culture medium, carrying out rejuvenation culture, carrying out composite culture of the three rejuvenated decolored bacterial in the broth culture medium to obtain a seed liquid, inoculating the seed liquid into a fermentation tank provided with the broth culture medium, and carrying out enlargement fermentation culture to prepare the efficient active composite corruption decoloring inoculant. The efficient active composite corruption decoloring inoculant has the advantages of simple preparation process, stable performances, use convenience, storage at normal temperature without inactivation, and realization of the bacterial dyed wastewater decoloring rate of 100%.

Description

technical field [0001] The invention provides a preparation method of a high-efficiency active compound putrefaction and decolorization bacterial agent, which belongs to the technical field of microbial preparations. Background technique [0002] At present, many countries and regions have developed a variety of high-efficiency microbial agents, which are made into commercial products for sale. The establishment of a microbial strain bank for environmental microbial preparations is the basis for promoting its industrial development to meet actual needs. The present invention is the application of microbial engineering in the field of environmental protection, especially the high-efficiency and active compound spoilage decolorization bacteria agent produced by fermentation technology is used to decolorize a large amount of bacterial dyeing wastewater produced every year in the microbiology laboratory. The main dyes in these wastewaters are basic dyes. They have a complex str...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/085C12R1/145C12R1/01
Inventor 张晓华周键王三反张学敏
Owner LANZHOU JIAOTONG UNIV
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