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Selective enterococcus enrichment medium

An enrichment medium, enterococcus technology, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as unsatisfactory effect and blackening, and achieve the effect of easier observation and obvious color change.

Active Publication Date: 2016-07-06
明儒成
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effect of the existing AC broth medium and enterococcus broth (all positive medium for hydrolyzed aescin) is not very satisfactory ( figure 1 , figure 2 )

Method used

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  • Selective enterococcus enrichment medium
  • Selective enterococcus enrichment medium
  • Selective enterococcus enrichment medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0031] Strains: (1) ATCC29212 was purchased from Qingdao Green Valley Trading Co., Ltd.

[0032] Reagents and media: (1) PROTEOSEPEPTONE (peptone, peptone) was purchased from MADEINENGLANDBYOXOIDLIMITEDLONDON.S.E.1; (2) Yeast extract powder was produced by Beijing Shuangxuan Microbial Medium Products Factory; (3) Beef extract Shanghai Milk Company Comprehensive Factory Production; (4) Glucose produced by Beijing Guohua Chemical Reagent Factory; (5) Lactose produced by Shanghai Chemical Reagent Factory; (6) Sodium Chloride produced by Zibo Chemical Reagent Factory; (7) Sodium Azide did not indicate the place of origin Label content: Art .822335NatriumazidZurSyntheseNaN3MERCK-schuchardtschuchardt,8011HohenbrunnbeiMǖnchenSehrgiftigVerschlucken.EntwickeltbeiBerǖhrungsauresehrgiftigeGase; (9) Nutrient broth medium is self-made in the laboratory; (10) AC broth medium is produced by Qingdao High-tech Park Haibo Biotechnology Co., Ltd.; (11) Plate colony counting agar culture

[0033...

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Abstract

The invention provides a selective enterococcus enrichment medium, the technical scheme of which is composed of the following components: protein powder, peptone 5.0g, yeast extract powder 2.5g, beef extract 3.0g, glucose 20.0g, lactose 4.0g, chlorine Sodium chloride 5.0g, sodium azide 2.5g, ox bile salt 10.0g, 0.1% bromocresol green 20% ethanol aqueous solution 6.0ml, distilled water 1000ml. Weigh 5.0g of peptone, 5.0g of peptone, 2.5g of yeast extract powder, 3.0g of beef extract, 20.0g of glucose, 4.0g of lactose, 5.0g of sodium chloride, 2.5g of sodium azide, and 10.0g of ox bile salt in a 1000ml Erlenmeyer flask In the above-mentioned Erlenmeyer flask, measure 1000ml of distilled water, heat to dissolve, adjust the pH value to 6.0, then add 6.0ml of 0.1% bromocresol green 20% ethanol aqueous solution, pack 50.0ml in a 150ml Erlenmeyer flask and wrap it in the temperature Sterilize for 15 minutes at 121°C. Beneficial effects of the present invention: compared with AC broth culture medium, the color change is more obvious, and the result is easier to observe.

Description

Technical field: [0001] The invention relates to the technical field of microbial culture medium, in particular to a selective enterococcus enrichment culture medium. Background technique: [0002] Recent studies have shown that most enterococci can produce bacteriocins, which are considered as opportunistic pathogens and can cause: urinary tract infection, abdominal cavity, pelvic cavity infection, wound infection after burns, etc. It is an important hospital infection second only to Staphylococcus pathogenic bacteria. Food poisoning caused by Enterococcus has been reported in my country in recent years, most of which are caused by Enterococcus faecalis and its variants, Enterococcus faecium, and Enterococcus gallinarum; food poisoning caused by Enterococcus saccharolyticum and Enterococcus avoid have been reported abroad. If the amount of enterococcus from clinical specimens is small or the enterococcus of food samples is damaged during the production process, it is not e...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 明儒成李汝期
Owner 明儒成
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