Preparation method and application of rapid detection kit for copper-containing nanorod compound
A detection kit and technology of copper nanorods are applied in the direction of material analysis, color/spectral property measurement, and chemical reaction of materials by observing the influence on chemical indicators, so as to achieve easy preparation and difficult inactivation. , the effect of good application prospects
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Embodiment 1
[0041] Firstly, copper-containing nanorod composites are prepared. Add 200 μL of 1M Na to 30 mL of the gold nanorod solution prepared by the seed crystal method 2 S 2 o 3 solution, placed in 50°C hot water and heated for 5 minutes to obtain core-shell gold nanorods, measure their corresponding absorption peaks on a UV-visible photometer, and the longitudinal plasma absorption peaks of gold nanorods red-shifted, continue to the solution Add 400μL 0.2M Cu(NO 3 ) 2 Solution, the longitudinal plasmon absorption peak of core-shell nanorods will blue-shift again to obtain copper-containing nanorod composites, such as figure 1 As shown in , after acting for 12 hours, centrifuge at 8000 rpm for 10 minutes on a centrifuge, remove the supernatant, and disperse with double distilled water for later use.
[0042] Prepare 14 centrifuge tubes, add 300 μL copper-containing nanorod complex to the centrifuge tubes, and then add 300 μL 5 mmol / L TMB and 300 μL 10 mmol / L H 2 o 2 , and then...
Embodiment 2
[0045] For the detection of glucose:
[0046] Add 200 μL of 1M Na to 30 mL of the gold nanorod solution prepared by the seed crystal method 2 S 2 o 3 solution, placed in hot water at 50°C for 5 minutes, and then added 400 μL of 0.2M Cu(NO 3 ) 2 Solution, after acting for 12 hours, centrifuge with 8000 rpm for 10 minutes on a centrifuge, remove the supernatant, and disperse it with twice distilled water for subsequent use. Add 300 μL of glucose solutions with concentrations of 0, 0.001, 0.01, 0.02, 0.4, 0.8, 2, 4, and 8 mM and 300 μL of 0.1 mg / mL glucose oxidase into 9 centrifuge tubes, and place them at 37°C for 30 minutes. Then add 300 μL copper-containing nanorod complex and 300 μL 5mmol / LTMB respectively, and finally adjust the pH to about 6 with 0.1mol / L HCl, and the color change is as follows: Figure 5 shown. After the color development is stable, measure its absorption spectrum on an ultraviolet spectrophotometer, and the ultraviolet absorption spectra of glucose ...
Embodiment 3
[0048] For the detection of insulin content:
[0049] Add 200 μL of 10 to 10 mL of copper-containing nanorod complex -4 μmol / L INS-aptamer, acted at 4°C for 12h, centrifuged at 4000rpm for 10min, re-dispersed with twice distilled water, and set aside.
[0050] Add 100 μL of the above-mentioned copper-containing nanorod complexes connected with INS-aptamer to each of the 8 centrifuge tubes, and then add 300 μL of the concentration of 0 and 10 -9 、10 -8 、10 -7 、10 -6 、10 -5 、10 -4 、10 -3 mg / mL insulin, after reacting for 20 minutes, add 200 μL of 10 mmol / L hydrogen peroxide solution for 30 minutes, and then add 300 μL of 5 mmol / L TMB respectively. After 30 minutes, its color changes as Figure 9 shown. The quantitative detection of insulin was performed in parallel three times, and the concentrations from left to right were: 0, 0.014, 0.054, 0.216, 0.270, 0.540, 2.16μU / mL, and the color changes as follows: Figure 10 shown. Under the conditions of different concentrat...
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