Method for accurately detecting toxicity of water quality by using photobacterium toxicity test

A technology of toxicity test and luminescent bacteria, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of comprehensive toxicity obstacles in luminescent bacteria toxicity test, and achieve high sensitivity, accurate results, and less time-consuming effects

Active Publication Date: 2012-09-05
济南市供排水监测中心
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

For this reason, the luminescent bacteria toxicity test has been greatly hindered in the detection of comprehensive toxicity in water.

Method used

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  • Method for accurately detecting toxicity of water quality by using photobacterium toxicity test

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Effect test

Embodiment 1

[0022] Taking Vibrio Qinghai as an example, the luminescence inhibition rate of the same substance was detected when it was pretreated and not treated:

[0023] Adopt the method of the present invention to prepare Qinghai Vibrio bacteria suspension: Qinghai Vibrio is cultured at 22 DEG C for 24 hours on LB solid medium, 30 single colony strains are inoculated in 150ml liquid LB medium, 22 DEG C shake culture 20h ; The liquid culture medium containing Vibrio qinghai was centrifuged under the centrifugation condition of 4000r / min, and then suspended with sterile normal saline to obtain a bacterial suspension with an OD value of 0.9;

[0024] Untreated Vibrio Qinghai was prepared directly into a bacterial suspension with an OD of 0.9.

[0025] Mix 0.1mg / L of chloroform, 0.02mg / L of deltamethrin, 0.02mg / L of carbon tetrachloride, and 0.25 mg / L of iron in the bacterial suspension at a volume ratio of 1:1, and detect after exposure for 10 minutes Luminescence inhibition rate, the r...

Embodiment 2

[0029] This specific embodiment adopts the following steps:

[0030] (1) Cultivate Vibrio Qinghai on LB solid medium at 22°C for 24h, inoculate 30 single colony strains into 150ml liquid LB medium, and culture with shaking at 22°C for 20h;

[0031] (2) Centrifuge the liquid medium containing luminescent bacteria under the centrifugation condition of 4000r / min, and then suspend it with sterile saline to obtain a bacterial suspension with an OD value of 0.9;

[0032] (3) Collect the factory water of the daily urban water plant, set up 3 parallels, mix the bacterial suspension and the water sample at a ratio of 1:1, control the exposure and reaction time of the bacteria at 10 minutes, and measure the luminescence after 10 minutes of reaction According to the determination, the luminescence inhibition rates of the three parallel samples of the water samples to be tested were 31.2%, 29%, and 30.7%, respectively, and the average luminescence inhibition rate was 30.3%.

[0033] Usin...

Embodiment 3

[0035] This specific embodiment adopts the following steps:

[0036] (1) Cultivate Vibrio Qinghai on LB solid medium at 20°C for 22 hours, inoculate single colony strains into liquid LB medium, and culture with shaking at 25°C for 16 hours. 5 -10 6 Between;

[0037] (2) Centrifuge the liquid medium containing luminescent bacteria under the centrifugation condition of 3500r / min, and then suspend it with sterile saline to obtain a bacterial suspension with an OD value of 0.7;

[0038] (3) Collect daily drinking water, set up 3 parallels, mix the bacterial suspension and the water sample at a ratio of 1:1, control the exposure reaction time of the bacteria at 15 minutes, and measure the luminescence after 15 minutes of reaction. The luminescence inhibition rates of the three parallel samples of the water sample to be tested were 42.4%, 43.5%, and 42.2%, respectively, and the average luminescence inhibition rate was 42.7%.

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Abstract

The invention discloses a method for accurately detecting toxicity of water quality by using a photobacterium toxicity test, and the method comprises the following steps: carrying out solid culture on photobacterium in an LB (lysogeny broth) solid culture medium, inoculating the obtained photobacterium strain into a liquid LB culture medium, and culturing; centrifuging the liquid culture medium containing photobacterium, and carrying out suspension by using aseptic normal saline to obtain a bacterium suspension with the OD (Optical Density) value of 0.4-0.9; and mixing a water sample to be detected with the bacterium suspension, and carrying out the photobacterium toxicity test to detect the photobacterium inhibition rate of the water sample to be detected. In the method disclosed by the invention, the photobacterium is processed, so that both organic toxic substances and inorganic toxic substances can inhibit the luminous intensity of the photobacterium, thereby enhancing the toxicity detection accuracy of water quality by using the photobacterium toxicity test, and solving the problem that the photobacterium can not properly reflect the comprehensive toxicity of the water quality. The method disclosed by the invention has the advantages of low time consumption, high sensitivity and accurate result, and is simple and convenient to operate.

Description

technical field [0001] The invention relates to a water quality toxicity detection method, in particular to a method for accurately detecting the toxicity of organic substances and inorganic substances in water by using a luminescent bacteria toxicity test. Background technique [0002] In order to ensure drinking water safety and reduce the threat of environmental degradation to drinking water quality, people often use various methods to evaluate water quality to effectively grasp the health risks caused by drinking water. [0003] The toxicity test of luminescent bacteria is one of the classic comprehensive biological toxicity evaluation methods. Due to the short growth period of bacteria and their sensitivity to environmental changes, the change degree of their luminous intensity is theoretically linearly related to the concentration of toxic substances. Bacterial tests represented by luminescent bacteria have been widely used at home and abroad, and many countries have a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18
Inventor 贾瑞宝宋艳马中雨孙韶华
Owner 济南市供排水监测中心
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