Method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442

Pseudomonas putida, the technology of the second step, applied in the biological field, can solve the problems of complex PHA method and high cost, and achieve the effects of strong product decomposition performance, low cost and good application prospect

Inactive Publication Date: 2013-07-24
SHANGHAI MEDICAL INSTR COLLEGE
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Problems solved by technology

[0006] However, the method for preparing PHA in the pr

Method used

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  • Method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442

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Embodiment

[0027] The present embodiment provides a method for synthesizing PHA with Pseudomonas putida KT2442, comprising the steps of:

[0028] Step 1: Take 1g of Pseudomonas putida KT2442 and mix it in 100g of LB medium. The culture temperature is 30°C, and cultured for 24 hours to obtain a seed culture solution containing bacteria. The composition of the LB medium is shown in Table 1:

[0029] Step 2, add 1g of the first group of culture fluid and 0.15g of octanoic acid and the second group of 1g of culture fluid and 1.5g of glucose into 100g of this medium for cultivation, and add 0.2g of the first group after 18 hours Octanoic acid, after 24 hours, add 0.15g octanoic acid to cultivate, separate, quick-freeze, dry, take the dried 1g PHA into 60ml chloroform to dissolve, filter, and heat at 30°C to obtain crude PHA. Crude PHA was added to 60ml of acetone and methanol mixture, heated at 30°C, and cultivated for 72 hours to obtain the final product PHA, wherein the composition of the c...

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Abstract

The invention provides a method for synthesizing PHA (Phytohemagglutinin) by using pseudomonas putida KT2442. The method comprises the following steps of: 1, mixing a small quantity of pseudomonas putida KT2442 in an LB (lysogeny broth) culture medium, and culturing to obtain a seed culture solution containing strains; and 2, adding the seed culture solution obtained in the step 1 and a carbon source to the culture medium, culturing, separating, quick-freezing, drying, and refining to obtain a final product PHA. According to the method, the pseudomonas putida KT2442 in culture wastewater is used, the supply of the carbon source is adjusted, and correlated matrix octane acid and non-correlated matrix glucose are respectively added in the culturing process, thus the final product PHA is obtained. The product obtained by using the method has strong decomposability, low glass transition temperature, good flexibility and adhesion and can be applied to the medicine field, and therefore, the production has a bright application prospect.

Description

technical field [0001] The invention relates to a method for preparing PHA in the biological field, in particular to a method for synthesizing PHA with Pseudomonas putida KT2442. Background technique [0002] Polyhydroxyalkanoates (PHA, polyhydroxyalkanoates): It is a biopolymer material that has developed rapidly in the past 20 years - polyhydroxyalkanoate (PHA). It is a kind of intracellular polyester synthesized by many microorganisms. It is a natural polymer biomaterials. Because PHA has good biocompatibility, biodegradability and thermal processing performance of plastics at the same time. Because it can be used as biomedical materials and biodegradable packaging materials at the same time, it has become the most active research hotspot in the field of biomaterials in recent years. PHA also has many high value-added properties such as nonlinear optics, piezoelectricity, and gas barrier properties. [0003] Natural or synthetic biodegradable high-strength materials te...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/40
Inventor 王志雄
Owner SHANGHAI MEDICAL INSTR COLLEGE
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