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Microbial insecticide for preventing and controlling aphids and preparation method of microbial insecticide

A technology for microbial insecticides and aphids, which is applied in the field of pest biological control, can solve problems such as loss, environmental pollution, and shortages, and achieve the effect of improving efficiency.

Inactive Publication Date: 2020-11-06
SHANDONG SUNWAY LANDSCAPE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aphid adults and nymphs gather on the back of crop leaves and tender stems to suck juice and secrete honeydew, which affects crop yield and quality, causing serious losses
Chemical pesticide control is a common measure to control aphids at this stage, but long-term use of chemical pesticides is a common measure to control aphids at this stage, but long-term use of chemical pesticides will cause aphids to develop resistance, and systemic insecticides can be absorbed by plants. It kills a lot of insects, but it can also kill beneficial insects, destroy the ecosystem and pollute the environment
Biological control is further divided into ecological control of natural enemies and microbial pesticide control. Among them, ecological control of natural enemies and the use of natural enemy insects of aphids such as ladybugs, hoverflies, lacewings, aphids, spiders, etc. Insect control, microbial pesticide control, including agricultural antibiotics and live microbial pesticides, live microbial control, control insects with bacteria, its biggest advantage is strong selectivity, safe for humans, animals, crops and the natural environment, does not harm natural enemies, and is not easy to produce resistance. Beauveria bassiana and Metarhizium anisopliae are commonly used in the control of biological pesticides, and there are few research reports on other fungi

Method used

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  • Microbial insecticide for preventing and controlling aphids and preparation method of microbial insecticide

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Experimental program
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Effect test

Embodiment 1

[0016] A kind of microbial insecticide for preventing and treating aphids, microbial insecticide is obtained by solid activation culture of strains, bacterial strains include: 25% of Neoaphididae mold, 15% of Acinetobacter baumannii, 18% of Bacillus thuringiensis, Bacillus amyloliquefaciens 16%, Bacillus subtilis 14%, Serratia 12%.

[0017] The microbial insecticide for controlling aphids of the present invention is prepared according to the following method:

[0018] (1) Introduce P. neophidii and Acinetobacter baumannii into the liquid seed activation medium for activation, and then insert 6% of the inoculum into the expansion medium for primary culture. The concentration obtained from the cultivation is (1-4)×10 8 CFU / ml, and then the bacterial solution obtained from the primary culture was inserted into another expansion medium with an inoculation amount of 7% for secondary culture to obtain a concentration of (5-10)×10 9 CFU / ml;

[0019] (2) Insert Bacillus thuringiens...

Embodiment 2

[0025] A kind of microbial insecticide that is used for preventing and treating aphids, microbial insecticide is obtained by the solid activation culture of strain, bacterial strain comprises: 22% of new aphids Placiformis, 20% of Acinetobacter baumannii, 14% of Bacillus thuringiensis, Bacillus amyloliquefaciens 13%, Bacillus subtilis 15%, Serratia 16%.

[0026] The microbial insecticide for controlling aphids of the present invention is prepared according to the following method:

[0027](1) Introduce P. neoaphidii and Acinetobacter baumannii into the liquid seed activation medium for activation, and then insert 8% of the inoculum into the expansion medium for primary culture. The concentration obtained from the cultivation is (1-4)×10 8 CFU / ml, and then the bacterial solution obtained from the primary culture was inserted into another expansion medium with a 5% inoculation amount for secondary culture to obtain a concentration of (5-10)×10 9 CFU / ml;

[0028] (2) Insert Ba...

Embodiment 3

[0034] A kind of microbial insecticide that is used for preventing and treating aphids, microbial insecticide is obtained by the bacterial strain solid activation culture, and bacterial strain comprises: 28% of new aphidia mold, 17% of Acinetobacter baumannii, 12% of Bacillus thuringiensis, Bacillus amyloliquefaciens 17%, Bacillus subtilis 10%, Serratia 16%.

[0035] The microbial insecticide for controlling aphids of the present invention is prepared according to the following method:

[0036] (1) Introduce P. neophidii and Acinetobacter baumannii into the liquid seed activation medium for activation, and then insert 5% of the inoculum into the expansion medium for primary culture. The concentration obtained from the cultivation is (1-4)×10 8 CFU / ml, and then the bacterial solution obtained from the primary culture was inserted into another expansion medium with an inoculation amount of 7% for secondary culture to obtain a concentration of (5-10)×10 9 CFU / ml;

[0037] (2) ...

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Abstract

The invention discloses a microbial insecticide for preventing and controlling aphids. The microbial insecticide is obtained by activation culturing of solid strains. The strains include 22-28% of Pandora neoaphidis strain, 15-20% of acinetobacter baumannii, 12-18% of bacillus thuringiensis, 13-18% of bacillus amyloliquefaciens, 10-15% of bacillus subtilis, and 12-16% of serratia. The microbial insecticide has the beneficial effects that by using the symbiotic relationship of Pandora neoaphidis strain and acinetobacter baumannii and the mutual cooperative effects of acinetobacter baumannii, bacillus thuringiensis, bacillus amyloliquefaciens, bacillus subtilis and serratia, Pandora neoaphidis strain helps acinetobacter baumannii, bacillus thuringiensis, bacillus amyloliquefaciens, bacillussubtilis and the like to invading into cells of aphids, and finally the aphids are sick, so that the efficiency of killing insects is improved.

Description

technical field [0001] The invention belongs to the field of pest biological control, in particular to a microbial insecticide for controlling aphids and a preparation method thereof. Background technique [0002] Aphids are an important group in Homoptera Rhozomorpha, which have many types, large numbers, fast reproduction speed, and serious damage. Aphids are also important vector insects that spread plant virus diseases. So far, the world's known aphid species There are nearly 4,000 species, most of which are crop pests. Adults and nymphs of aphids gather on the back of leaves and tender stems of crops to suck juice and secrete honeydew, which affects crop yield and quality and causes serious losses. Chemical pesticide control is a common measure to control aphids at this stage, but long-term use of chemical pesticides is a common measure to control aphids at this stage, but long-term use of chemical pesticides will cause aphids to develop resistance, and systemic insect...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/23A01N63/22A01N63/20A01N63/30A01P7/04C12R1/07C12R1/125C12R1/425C12R1/645C12R1/01
CPCA01N63/20A01N63/22A01N63/23A01N63/30C12N1/14C12N1/20
Inventor 王胜
Owner SHANDONG SUNWAY LANDSCAPE TECH
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