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Composite bacteria preparation for reducing ammonia release and preserving nitrogen in cow dung compost

A compound bacterial agent and ammonia release technology, applied in the field of microorganisms, to achieve broad application prospects, increase the content of nitrogen elements, and reduce ammonia pollution

Active Publication Date: 2012-03-28
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem of NH in the composting fermentation process that has not yet been found to be used to reduce cow dung as the main raw material. 3 Volatility and nitrogen retention of biological agents, and provide a compound bacterial agent for cow manure composting to reduce ammonia release and nitrogen retention

Method used

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Examples

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Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific embodiment one: the composite bacterial agent that is used for cow dung composting to reduce ammonia release and nitrogen retention of the present embodiment consists of 12 parts of Serratia plymouth ( Serratia plymuthica ) preservation number ACCC No.01503 fermentation broth, 17 copies of Sphingomonas ( Sphingomonas trueperi ) preservation number ATCC No.12417 fermentation broth, 50 copies of Alcaligenes ( Alcaligenes sp. ) preservation number DSM No.2625 fermentation broth composition; the number of effective viable bacteria in the fermentation broth of the above three bacteria is 10 9 Individuals / mL or more.

[0011] The preparation method of this composite bacterial agent is as follows:

[0012] (1) Activation of strains: the preserved Serratia plymouth ( Serratia plymuthica ), Sphingomonas ( Sphingomonas trueperi ) and Alcaligenes ( Alcaligenes sp. ) were respectively inoculated into 150mL Erlenmeyer flasks containing 50mL of activation medium, th...

specific Embodiment approach 2

[0017] Specific embodiment two: the compound bacterial agent that is used for cow dung composting of the present embodiment to reduce ammonia release and nitrogen retention is made of 20 parts of Serratia plymouth ( Serratia plymuthica ) preservation number ACCC No.01503 fermentation broth, 25 copies of Sphingomonas ( Sphingomonas trueperi ) preservation number ATCC No.12417 fermentation broth, 63 copies of Alcaligenes ( Alcaligenes sp. ) preservation number DSM No.2625 fermentation broth composition; the number of effective viable bacteria in the fermentation broth of the above three bacteria is 10 9 Individuals / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as the first embodiment.

[0018] Inoculate the composite bacterial agent of this embodiment into compost mixed with cow dung and crushed corn stalks at a volume ratio of 2:1, and carry out compost fermentation for 60 days. NH 3 The peak of the release amount appear...

specific Embodiment approach 3

[0020] Specific embodiment three: the present embodiment is used for cow dung composting to reduce the release of ammonia and the composite bacterial agent of nitrogen retention by 13 parts of Serratia plymouth ( Serratia plymuthica ) preservation number ACCC No.01503 fermentation broth, 18 copies of Sphingomonas ( Sphingomonas trueperi ) preservation number ATCC No.12417 fermentation broth, 53 copies of Alcaligenes ( Alcaligenes sp. ) preservation number DSM No.2625 fermentation broth composition; the number of effective viable bacteria in the fermentation broth of the above three bacteria is 10 9 Individual / mL or more. The preparation method of the composite bacterial agent of this embodiment is the same as the first embodiment.

[0021] Inoculate the composite bacterial agent of this embodiment into compost mixed with cow dung and crushed corn stalks at a volume ratio of 2:1, and carry out compost fermentation for 60 days. NH 3 The peak of the release amount appeared o...

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Abstract

The invention relates to a composite bacteria preparation for reducing ammonia release and preserving nitrogen in cow dung compost and belongs to the field of microorganisms. The invention aims at solving the problem that the existing biological preparation for reducing the volatile quantity of NH3 in the compost fermentation process through taking cow dungs as main raw materials and preserving the nitrogen is not invented. The composite bacteria preparation comprises the following components in part by weight: 12-20 parts of Serratiaplymuthica fermentation liquor with a collection number of ACCC No. 01503, 17-25 parts of Sphingomonastrueperi fermentation liquor with a collection number of ACCC No. 12417 and 50-63 parts of Alcaligenessp fermentation liquor with a collection number of DSM No. 2625. The composite bacteria preparation can greatly reduce the release amount of the NH3 in the composting process and effectively improve the content of nitrogen elements in a fertilizer.

Description

technical field [0001] The invention belongs to the field of microbes, and in particular relates to a composite bacterial agent for reducing ammonia release and maintaining nitrogen in cow dung composting. Background technique [0002] With the continuous development of my country's large-scale livestock and poultry breeding industry, the production of livestock and poultry manure is also increasing. If a large amount of livestock and poultry manure is not treated in a harmless manner after production, it will not only produce malodorous gas, but also cause serious environmental pollution. Pollution can also breed mosquitoes and lead to the widespread spread of pathogenic bacteria, which will eventually threaten human health. Therefore, people must carry out harmless treatment to livestock and poultry manure. At present, composting technology is the most commonly used and most effective method for the harmless treatment of livestock and poultry manure. Composting technology...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/425C12R1/05C12R1/01
CPCY02W30/40
Inventor 王伟东王彦杰陈文浩孙志远高亚梅刘权
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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