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Microbial fermentation functional organic compound fertilizer and production method thereof

A microbial fermentation and production method technology, which is applied in the field of microbial fermentation functional organic compound fertilizer and its production, can solve the problems of poor survival of effective microorganisms and short shelf life, etc., to improve germination rate and crop stress resistance, improve shelf life, The effect of improving disease resistance

Inactive Publication Date: 2014-04-16
孙震海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In addition, the current biological fermentation fertilizer has a short shelf life and poor survival effect of effective microorganisms; while the number of bacteria in microbial fermented organic fertilizers is maintained, the length of bacterial survival is an important indicator of microbial fertilizers, and the survival time of screened strains is long. Strains with strong activity, adaptability to the environment and strong ability to survive and colonize are the key technology for the production of bio-organic fertilizers and the basic guarantee for large-scale production of bio-organic fertilizers

Method used

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  • Microbial fermentation functional organic compound fertilizer and production method thereof
  • Microbial fermentation functional organic compound fertilizer and production method thereof
  • Microbial fermentation functional organic compound fertilizer and production method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Cultivation of primary strains

[0039] The percentage by weight of each component of the primary strain culture medium is:

[0040] Industrial whey powder 2%, industrial sodium lactate 0.4%, industrial anhydrous sodium acetate 0.1%, industrial peptone 1%, soybean meal powder 3%, industrial glucose 1.5%, yeast extract powder 0.5%, tap water 91.5%, pH natural, above Mix and stir to dissolve.

[0041] Specific operation: first take a 500ml triangular flask, fill each bottle with 200ml of the above-dissolved culture medium, then seal the triangular flask with 6-8 layers of gauze, wrap it with a layer of kraft paper, and put it into a medical pressure sterilizer for steam sterilization; first Turn on the heating source of the sterilizer, and when the steam pressure in the sterilizer reaches 0.05 MPa, open the exhaust valve on the sterilizer to discharge cold air for 6-8 minutes; when the pressure gauge on the sterilizer returns to normal At zero hour, close the exhaust va...

Embodiment 2

[0051] Utilize the method for above-mentioned seed tank cultivating bacterial classification to ferment biological organic fertilizer, concrete steps are as follows:

[0052] The formula is as follows: Take 100kg fertilizer as an example, excluding water and bacteria

[0053] Dry chicken manure 30kg, dry pig manure 53.4kg, 40 mesh fine rice husk powder 10kg, lactose 0.1kg, chitosan oligosaccharide 0.5kg, wheat bran 6kg, weigh and mix all materials, add 40kg of tap water and mix well, pile up and park for 5 hour, then add 2wt% of the above-cultured seed tank bacterial classification (2wt% of the dry material), first add after adding by diluting with tap water in a ratio of 1:3, both 1kg of bacteria liquid and 3kg of tap water, turn over while adding , add the bacteria and mix well and start stacking. It needs to be stacked into a long stack with a bottom width of 1.5 meters, an upper width of 1.2 meters, a height of 1.2 meters, and an unlimited length. The cross-section of the...

experiment example 4

[0075] After the inventor added lactose and chitosan, the impact on the final result was compared, and the results are as follows:

[0076] Comparison table 4 of trehalose produced by active dry yeast with added lactose

[0077]

[0078] Adding chitosan oligosaccharide in production improves the effect of Lactobacillus brevis on degrading hormones, and also improves the effect of Bacillus cereus on degrading drug residues.

[0079] Determination effect of estrogen in organic fertilizer degraded by Lactobacillus brevis Table 5

[0080]

[0081]

[0082] Add the assay result table 6 of the bacterial classification fermentation organic fertilizer hormone of chitosan oligosaccharide

[0083]

[0084] Test results of Bacillus cereus degrading drug residues in organic fertilizer Table 7

[0085]

[0086] Table 8

[0087]

[0088] It can be seen that adding lactose and chitooligosaccharides to the cultured strains can make the strains produce the required effecti...

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Abstract

The invention relates to a microbial fermentation functional organic compound fertilizer and a production method thereof. The method comprises the following steps of: mixing lactobacillus buchneri (i) Lactobacillusbuchneri ( / i) CICC (China Center of Industrial Culture Collection) 6004, bacillus cereus (i) Bacilluscereus ( / i) ACCC (Agricultural Culture Collection of China) 10117, saccharomycescerevisiae (i) Saccharomyces ( / i) (i) cerevisiae ( / i) CICC1905, and serratia marcescent (i) Serratia ( / i) (i) marcescent ( / i) ACCC10119; and fermenting to produce an bio-organic fertilizer. With the adoption of the mixing-fermenting method, the estrogen and medicine residues in excrements of livestocks can be effectively degraded; nematode and fly maggot in the soil can also be eliminated; in addition, the shelf life of the organic fertilizer and the survival time of viable organism can be greatly prolonged, and the germination rate and stress resistance of crops seeds can also be improved, etc.

Description

technical field [0001] The invention relates to the field of bioengineering, and relates to a microbial fermentation functional organic compound fertilizer and a production method thereof. Background technique [0002] The development of modern agriculture attaches great importance to the development of ecological agriculture, and high-yield and high-quality crop cultivation techniques are inseparable from fertilizers. "A single flower in agriculture depends entirely on fertilizer". The rational use of organic fertilizers plays an increasingly important role in modern agricultural production, and organic agriculture has become the direction of agricultural development in the future. We know that the rapid development of agriculture is inseparable from the use of chemical fertilizers, but due to the long-term abuse of chemical fertilizers in agricultural production, soil compaction, environmental pollution and decline in the quality of agricultural products have also led to ...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05F17/00C05F15/00
CPCY02W30/40
Inventor 孙震海
Owner 孙震海
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