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Preparation method of microbial flora preparation and application thereof

A microbial flora and preparation technology, applied in the fields of soil improvement, microorganisms, water treatment, and composting, can solve problems such as limited cognition of natural flora, achieve obvious effects, wide application fields, and easy to use

Inactive Publication Date: 2016-02-24
金龙 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although the composition of natural microbial flora is complex, people often use several, a dozen or dozens of species to characterize this flora. One of the reasons is that people's knowledge of all natural flora is still very limited

Method used

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  • Preparation method of microbial flora preparation and application thereof
  • Preparation method of microbial flora preparation and application thereof
  • Preparation method of microbial flora preparation and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Disposal methods of agricultural product processing waste

[0025] In the present invention, five types of agricultural product processing wastes, namely onion waste, wheat bran, rice bran, discarded figs, and bean processing waste, are used as raw materials. Onion waste includes onion skins, onion roots, spoiled onions, onion residues, etc. Bean processing waste mainly uses bean curd residue and industrial bean cake. The relative content of the 5 wastes of the present invention does not need to be fixed in particular, but generally includes 20-30% onion waste, 25% wheat bran bran, 30-40% rice bran, 10% discarded figs, 5% of pulse processing waste. The above raw materials are usually stored in a ventilated and cool place at room temperature, and only mixed in a certain proportion before use and crushed immediately until the final average particle diameter is not greater than 2 mm.

Embodiment 2

[0027] Preparation and Preservation Method of Bacteria Preparation Mother Bacteria (koji powder)

[0028] 7 parts of freshly mixed and pulverized agricultural product processing waste plus 3 parts by weight of water are uniformly mixed and fermented in an open container at a constant temperature of 37° C. for 48-72 hours. In order to properly accelerate the speed and degree of fermentation, a certain amount (0.01-0.1%) of stock koji powder can also be appropriately added in this step. After the fermented product is naturally ventilated and dried or heated and dried, it becomes koji powder. The water content of koji powder is between 5-15%, and it can be stored in a cool and airtight place at room temperature for a long time. Koji powder should not be prepared frequently in small quantities, but needs to be prepared on a large scale. The quantity prepared at one time is preferably enough to be used for more than 5 years, so that the natural flora species of the original koji po...

Embodiment 3

[0030] Method for large-scale preparation of liquid bacterial preparation by using koji powder

[0031] The scale of preparation of the liquid bacterial preparation is not particularly limited. For example, it can be prepared in a 1-ton container, a 5-ton container or a 10-ton container. For example: add 10 kg of koji powder, 20 kg of sugarcane molasses, 1000 g of red ginseng beard powder (the particle diameter after crushing is not greater than 2 mm) into a 1-ton container, add water to a total volume of about 1000 liters, and stir for 20-30 Cultivate at ℃ for 72 hours, during which an aeration machine (power 3500 watts) is used to stir and oxygenate for 1 hour every 4 hours; the stirring speed is 120 revolutions per minute. In this way, a liquid composite bacteria preparation is obtained.

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Abstract

The invention discloses a preparation method of a microbial flora preparation and application thereof. The preparation method comprises the steps of fermenting five agricultural product processing wastes such as onion processing wastes, soybean processing wastes, rice bran, wheat bran and abandoned fig at 37 DEG C to prepare mother bacteria yeast powder, the obtained yeast powder contains pediococcus acidilactici, lactobacillus plantarum, lactobacillus pentosus, lactobacillus paracasei, agrobacterium tumefaciens, Serratia, lactobacillus fermenti, agrobacterium, iron base autotrophic nitrifying bacteria, cucumber stem xanthomonas ochrobactrum anthropi, lactobacillus casei, nasturtium fructose bacillus, bacillus aceticus and the like. The yeast powder can be put into bags for long-term preservation after being dried. The yeast powder, molasses, red ginseng tails and water are stirred and cultured for 72 hours by introducing oxygen to prepare a liquid compound bacteria preparation, and the liquid bacteria preparation can be preserved for one year under room-temperature and shady and cool conditions. The microbial flora preparation can be used when attenuation by water before being used, and the effects on aspects of cultivation, compost treatment, wastewater treatment, soil improvement and the like are obvious.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a method for preparing a microbial flora preparation and the microbial flora preparation and its application. The flora comes from the mixed semi-solid fermentation of onions and a batch of agricultural crop processing waste. The prepared flora preparation can be widely applied to the fields of soil improvement, water treatment, composting treatment and the like. Background technique [0002] There are a large number of natural microbial communities or flora in nature. For example, the microbial flora in seawater can include thousands of different microorganisms; the microbial flora in wine cellars generally contains hundreds to thousands of microorganisms; Including more than 1,000 different microorganisms, of which about 110 species are relatively high; about half of the species with the highest content in the natural microbial flora may belong to unknown bacteri...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00C02F3/34C05F11/08C12R1/25C12R1/225C12R1/425C12R1/41C12R1/245C12R1/02C12R1/01
Inventor 金龙金勇智
Owner 金龙
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