Compound microorganism preparation, preparing method and application of compound microorganism preparation to processing animal carcasses
A technology of compound microorganisms and preparations, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, animal carcass fertilizers, etc., can solve the problems of anoxic decay of plants, pollution of the surrounding environment, high oil content of organic fertilizers, and reduce odor. Concentration, good synergy, effect of high deodorization function
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Embodiment 1
[0042] A compound microbial preparation provided in this example comprises Bacillus stearothermophilus, Pseudomonas aeruginosa, Streptococcus thermophilus, Bacillus megaterium and Bacillus flexus.
[0043] The present embodiment also provides the preparation method of above-mentioned compound microbial preparation, comprises the steps:
[0044] (1) Activated bacteria
[0045] Each strain was inoculated on the slant medium corresponding to each strain for cultivation, among which Bacillus stearothermophilus (Bacillus stearothermophilus) CGMCC 1.1923, Pseudomonas aeruginosa (Pseudomonas aeruginosa) CICC 23683, Bacillus megaterium ) ACCC 01509 and Bacillus flexus (Bacillus flexus) ACCC 02938 were inoculated on LB medium, and cultured at 30°C for 24 hours; Activation of various strains;
[0046] (2) Erlenmeyer shake flask culture
[0047] Inoculate each bacterial strain activated in step (1) onto the corresponding Erlenmeyer flask liquid culture medium of each bacterial strain ...
Embodiment 2
[0062] A compound microbial preparation provided in this example comprises Bacillus stearothermophilus, Pseudomonas aeruginosa, Streptococcus thermophilus, Bacillus megaterium and Bacillus flexus.
[0063] The present embodiment also provides the preparation method of above-mentioned compound microbial preparation, comprises the steps:
[0064] (1) Activated bacteria
[0065] Each strain was inoculated on the slant medium corresponding to each strain for cultivation, among which Bacillus stearothermophilus (Bacillus stearothermophilus) CGMCC 1.1923, Pseudomonas aeruginosa (Pseudomonas aeruginosa) CICC 23683, Bacillus megaterium ) ACCC 01509 and Bacillus flexus (Bacillus flexus) ACCC 02938 were inoculated on LB medium, and cultured at 35°C for 24 hours; Activation of various strains;
[0066] (2) Erlenmeyer shake flask culture
[0067] Inoculate each bacterial strain activated in step (1) into the corresponding Erlenmeyer flask liquid culture medium of each bacterial strain ...
Embodiment 3
[0082] A compound microbial preparation provided in this example comprises Bacillus stearothermophilus, Pseudomonas aeruginosa, Streptococcus thermophilus, Bacillus megaterium and Bacillus flexus.
[0083] The present embodiment also provides the preparation method of above-mentioned compound microbial preparation, comprises the steps:
[0084] (1) Activated bacteria
[0085] Each strain was inoculated on the slant medium corresponding to each strain for cultivation, among which Bacillus stearothermophilus (Bacillus stearothermophilus) CGMCC 1.1923, Pseudomonas aeruginosa (Pseudomonas aeruginosa) CICC 23683, Bacillus megaterium ) ACCC 01509 and Bacillus flexus (Bacillus flexus) ACCC 02938 were inoculated on LB medium, and cultured at 40°C for 36 hours; Activation of various strains;
[0086] (2) Erlenmeyer shake flask culture
[0087] Inoculate each bacterial strain activated in step (1) onto the corresponding Erlenmeyer flask liquid culture medium of each bacterial strain ...
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