Immobilization pseudomonas aeruginosa as well as preparation method and application thereof

A technology of Pseudomonas aeruginosa and live bacteria, which is applied in biochemical equipment and methods, chemical instruments and methods, fixed on or in inorganic carriers, etc. To deal with the problem of high cost, to achieve the effect of good biocompatibility, good compressive strength and aeration resistance, and high activity

Inactive Publication Date: 2010-09-01
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hexavalent chromium compounds have a strong oxidation effect, are harmful to the human digestive tract, respiratory tract, skin and mucous membranes, and have carcinogenic effects on the lungs, and hexavalent chromium is difficult to degrade and easily bi

Method used

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  • Immobilization pseudomonas aeruginosa as well as preparation method and application thereof
  • Immobilization pseudomonas aeruginosa as well as preparation method and application thereof
  • Immobilization pseudomonas aeruginosa as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] a kind of like figure 1 , figure 2 The shown immobilized Pseudomonas aeruginosa of the present invention includes live Pseudomonas aeruginosa and a mixed carrier for immobilizing the live Pseudomonas aeruginosa, and the mixed carrier is an embedded Pseudomonas aeruginosa The spheres of living bacteria, the mixed carrier is mainly composed of polyvinyl alcohol, calcium alginate and multi-walled carbon nanotubes with a mass ratio of 60:40:4 (although there will be a slight difference in the ratio of the raw materials used, the difference can be can be ignored).

[0030] The above-mentioned immobilized Pseudomonas aeruginosa of the present embodiment is prepared by the following method:

[0031] 1. Preparation of immobilization reagent:

[0032] Mix 6g, 4g, and 0.4g of polyvinyl alcohol, sodium alginate, and multi-walled carbon nanotubes respectively, and dilute to 100mL with water to prepare a mixed solution; then heat the mixed solution to boiling, and stir continuou...

Embodiment 2

[0045] Transfer 20-30 g of the immobilized Pseudomonas aeruginosa prepared in the above-mentioned Example 1 to an Erlenmeyer flask containing 100 mL of the above-mentioned fermentation medium, and activate it at 37°C for two days to fully restore the activity of the immobilized Pseudomonas aeruginosa . Then add hexavalent chromium solutions with different initial concentrations, culture on a shaker at 37°C and 150 rpm, and take samples regularly. After samples are taken at regular intervals and centrifuged at 8000 rpm for 8 minutes (or filtered through a microporous membrane), the supernatant is taken for measurement and the supernatant is used for the determination of hexavalent chromium content. The absorbance was measured at 540 nm by diphenylcarbazide spectrophotometry. All utensils used in the determination were soaked overnight in dilute nitric acid, and then cleaned. In the determination, diphenylcarbazide is prepared with acetone and distilled water with a volume rat...

Embodiment 3

[0050] Using the immobilized Pseudomonas aeruginosa prepared in Example 1 to reduce the hexavalent chromium solution, the operation steps are: first add the immobilized Pseudomonas aeruginosa to 100 mL of fermentation medium for activation, generally for 1 day Above, then the hexavalent chromium solution to be treated is added to the fermentation medium after activating Pseudomonas aeruginosa, so that the concentration of hexavalent chromium is 15mg / L, and the immobilized Pseudomonas aeruginosa is controlled to contain hexavalent chromium The concentration in the fermentation medium was 0.1 g / mL, and the shaking treatment was carried out at 37° C. for 2 days, and the rotational speed during the shaking treatment was 150 rpm. Continue to use the immobilized Pseudomonas aeruginosa for four consecutive times to reduce the hexavalent chromium of 15mg / L. During the reduction process, the continuous use of the immobilized Pseudomonas aeruginosa is to filter the solution after the pre...

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Abstract

The invention belongs to the treatment field of heavy metal polluted wastewater and particularly discloses immobilization pseudomonas aeruginosa. The immobilization pseudomonas aeruginosa comprises pseudomonas aeruginosa live bacteria and a mixing carrier used for fixing the bacteria, wherein the mixing carrier is a spherical object embedded with the live bacteria and mainly comprises the following compositions of polyvinyl alcohol, calcium alginate and multi-wall carbon nano-tube in the mass ratio of 50-60:30-40:1.6-6. The preparation method of the immobilization pseudomonas aeruginosa comprises the following steps of: firstly, preparing a mixed solution by mixing the polyvinyl alcohol, the calcium alginate, the multi-wall carbon nano-tube and water, uniformly heating and stirring, and obtaining an immobilization reagent by standing; secondly, adding a pseudomonas aeruginosa into an immobilization reagent to obtain a bacteria solution, and dripping the bacteria solution into a calcium nitration solution to obtain an immobilization pseudomonas aeruginosa microsphere; and finally, obtaining the immobilization pseudomonas aeruginosa through frozen-thawed treatment. The immobilization pseudomonas aeruginosa can reduce hexavalent chromium to trivalent chromium, has the advantages of high efficiency, favorable effect, also simple operation and no secondary pollution.

Description

technical field [0001] The invention belongs to the field of heavy metal-polluted wastewater treatment, and in particular relates to an immobilized microorganism, a preparation method thereof and an application thereof in treating heavy metal-polluted wastewater. Background technique [0002] In the process of adapting to the environment, microorganisms can use various pollutants as nutrient sources to convert pollutants in the environment into stable, low-toxic or non-toxic substances through a series of reactions such as absorption and metabolism. By artificially strengthening such capabilities, microbial technology can be widely used in environmental protection. Today, microbial technology has been used in water pollution control, air pollution control, degradation of toxic and harmful substances, development of clean and renewable energy, waste recycling, and environmental protection. Various aspects of environmental protection, such as monitoring and remediation of poll...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12N11/10C12N11/08C12N11/04C02F3/34B09C1/10A62D3/02C12R1/385C02F101/22A62D101/43
Inventor 汤琳庞娅曾光明杨春平黄丹莲刘媛媛李贞刘灿
Owner HUNAN UNIV
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