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Complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and restoring method thereof

A compound bacterial agent and a technology for polluted soil, applied in the field of soil remediation, can solve the problems of long treatment period, poor treatment effect and high investment, and achieve the effects of improved degradation effect, remarkable treatment effect and simple process

Active Publication Date: 2012-11-28
PETROCHINA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the process of realizing the present invention, the inventors have found that the prior art has at least the following problems: the existing method has a long treatment cycle, is greatly affected by natural factors such as soil and temperature, and has relatively large limitations; the existing oil-degrading bacteria It is difficult to completely degrade the complex composition system of crude oil and other pollution components, and the environmental adaptability is limited, the investment is high, and the treatment effect is not good

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Preparation of microbial compound bacteria agent:

[0021] Pseudomonas aeruginosa, Pseudomonas chrysogenum, Acinetobacter ruficii, Nocardioid chrysogenum and Streptomyces chrysogenum are mixed according to the percentage by weight of 3:1.5:1:2:2 to obtain a microbial composite bacterial agent.

[0022] The method for repairing oil-contaminated soil in the loess plateau area using the described microbial compound bacterial agent comprises the following steps:

[0023] (1) Pretreatment of contaminated soil

[0024] Separate the impurities in the oil-contaminated soil and crush the soil particles with a particle size larger than 1cm to keep the soil moisture content at 30% and adjust the pH to 8.0;

[0025] (2) Degradation of polluted soil

[0026] Add urea and superphosphate in turn to the polluted soil, so that the ratio of soil C:N:P is 85:9:2, add crushed straw to 9% of the polluted soil weight, electron acceptor agent H 2 o 2 1.8% of the weight of the polluted soi...

Embodiment 2

[0030] Preparation of microbial compound bacteria agent:

[0031] Pseudomonas aeruginosa, Pseudomonas chrysogenum, Acinetobacter ruficii, Nocardioid chrysogenum and Streptomyces chrysogenum are mixed according to the weight percentage of 2:0.5:0.5:3:1.5 to obtain a microbial composite bacterial agent.

[0032] The method for repairing oil-contaminated soil in the loess plateau area using the described microbial compound bacterial agent comprises the following steps:

[0033] (1) Pretreatment of contaminated soil

[0034] Separate the impurities in the oil-contaminated soil and crush the soil particles with a particle size larger than 1cm to keep the soil moisture content at 25% and adjust the pH to 7.2;

[0035] (2) Degradation of polluted soil

[0036] Add urea and diammonium phosphate sequentially to the polluted soil, so that the ratio of soil C:N:P is 75:8:3, add crushed straw to 8.3% of the weight of the polluted soil, electron acceptor agent H 2 o 2 It is 1.5% of the...

Embodiment 3

[0040] Preparation of microbial compound bacteria agent:

[0041] Pseudomonas aeruginosa, Pseudomonas chrysogenum, Acinetobacter ruficii, Nocardioid chrysogenum and Streptomyces chrysogenum are mixed according to the percentage by weight of 5:2:1.5:3:3.6 to obtain a microbial composite bacterial agent.

[0042] The method for repairing oil-contaminated soil in the loess plateau area using the described microbial compound bacterial agent comprises the following steps:

[0043] (1) Pretreatment of contaminated soil

[0044] Separate the impurities in the oil-contaminated soil and crush the soil particles with a particle size larger than 1cm to keep the soil moisture content at 35% and adjust the pH to 7.5;

[0045] (2) Degradation of polluted soil

[0046] Add urea and superphosphate in turn to the polluted soil, so that the ratio of soil C:N:P is 85:9:2, add crushed straw to 10% of the polluted soil weight, electron acceptor agent H 2 o 2 It is 2.0wt% of the weight of the p...

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Abstract

The invention discloses a complex microbial inoculant for restoring petroleum-contaminated soil in loess highlands and a restoring method thereof. The microbial body of the complex microbial inoculant comprises Pseudomonas aeruginosa, Pseudomonas fulva, Acinetobacter lwoffii, Nocardioides luteus and Streptomyces flavoviridis. The invention provides a method for restoring petroleum-contaminated soil in loess highlands by using the complex microbial inoculant, which comprises the following steps of (1) carrying out pretreatment on contaminated soil; (2) degrading the contaminated soil; (3) covering a plastic film on the soil; and (4) adding water, urea, diammonium phosphate and the complex microbial inoculant. By using the method disclosed by the invention, the process is simple, the cost is low, and the degradation effect is significantly increased.

Description

technical field [0001] The invention relates to the field of soil remediation, in particular to a microbial composite bacterial agent for remediating oil-contaminated soil in a loess tableland and a remediation method thereof. Background technique [0002] At present, the bioremediation technology of domestic oily soil is mainly based on microorganism and phytoremediation technology, and the former includes in-situ and ex-situ remediation methods. In view of the characteristics of wide and scattered distribution of domestic oil fields, most of them adopt the ex-situ bioremediation technology of centralized stacking. Among them, the tillage treatment adopts measures such as fertilization, irrigation, lime application, and plowing to the polluted soil to improve the soil's aeration, moisture and pH conditions, and promote natural degradation. It is suitable for low-concentration oil-contaminated soil. At present, most of the microorganisms used in indoor experiments, pilot te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 张海玲毛怀新曾亚勤周立辉杨琴任建科冀忠伦陈丽华李岩赵敏张璇任小荣蒋继辉
Owner PETROCHINA CO LTD
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