Aeromonas veronii rapid detection primer, kit and application

Abstract

The invention discloses an aeromonas veronii rapid detection primer, a kit and application. The aeromonas veronii rapid detection primer is characterized by comprising an outer primer and an inner primer; the outer primer is composed of an outer primer upstream primer shown in SEQ ID NO.1 and an outer primer downstream primer shown in SEQ ID NO.2; the inner primer is composed of an inner primer upstream primer shown in SEQ ID NO.3 and an inner primer downstream primer shown in SEQ ID NO.4. According to the method, the period for detecting aeromonas veronii is short, the detection cost is low, sequencing and standardization of the detection process are achieved, operation is standard, and errors are not prone to being caused. The specificity is good. The dye built-in method is adopted, a reaction tube does not need to be opened after a reaction is finished, results can be observed directly with the naked eyes after the reaction tube is taken out, a follow-up to-be-detected sample is prevented from being polluted by amplified products, bacterial culture does not need to be carried out, and detection can be carried out only through fewer samples such as fish blood.

Description

technical field [0001] The invention relates to a rapid detection primer, kit and application of Aeromonas verkistan, belonging to the field of rapid detection of aquatic pathogens. Background technique [0002] Aeromonas veronii, also known as Aeromonas verona, Aeromonas vanlon, and Aeromonas veronii, is a genus of Gram-negative bacilli. It is a kind of Aeromonas discovered and identified in recent years. Aeromonas verdeii is an opportunistic pathogenic bacterium that affects humans and animals. In recent years, freshwater cultured fish, such as catfish, carp, koi, Chinese soft-shelled turtle, and river crab, have been infected by Aeromonas verdeii. Reports have brought huge economic losses to farmers. At present, for the detection of Aeromonas victorii in fish, traditional bacterial isolation and identification, serological reaction and PCR detection technology are mainly used. Bacterial isolation and identification is time-consuming and costly. Most of them are based on...

AI Technical Summary

Problems solved by technology

Aeromonas verdeii is an opportunistic pathogenic bacterium that affects humans and animals. In recent years, freshwater cultured fish, such as catfish, carp, koi, Chinese soft-shelled turtle, and river crab, have been infected by Aeromonas verdeii. Reports have brought huge economic losses to the farmers

At present, for the detection of Aeromonas victorii in fish, traditional bacterial isolation and identification, serological reaction and PCR detection technology are mainly used. Bacterial isolation and identification is time-consuming and costly. Most of them are based on the premise of killing aquatic animals, and the operation is complicated. Professional equipment and technical personnel are needed, and there is an urgent need for a rapid, accurate, and easy-to-operate detection technology and its products for Aeromonas victoria that can be applied to the farming site.

There is no report on the LAMP detection technology of Aeromonas verkisii

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