Multi-plant extraction method, staying-up glowing composition containing multi-plant extraction extract and preparation method of staying-up glowing composition

Abstract

The invention discloses a staying-up glowing composition containing multiple plant extracts. The staying-up glowing composition containing the multiple plant extracts is prepared from the following components in parts by mass: 3 to 5 parts of extract A, 10 to 15 parts of extract B, 2 to 4 parts of extract C, 1.5 to 3 parts of curcumin nano-liposome, 2 to 5 parts of lactobacillus fermentation filtrate, 1 to 3 parts of water-locking magnetite, 0.1 to 0.3 part of xanthan gum / acrylate compound thickening agent and 0.5 to 1 part of preservative composition. According to the staying up glowing composition containing the multiple plant extracts, the target component of the extract A is jasmonic acid methyl ester, the target component of the extract B is salidroside and nausea acid, and the curcumin nano-liposome is compounded, so that the staying up glowing composition containing the multiple plant extracts is obtained; compared with similar products sold in the market at present, the anti-oxidation and anti-saccharification capacities of the staying-up glowing composition can be enhanced in a targeted manner, the transdermal efficiency is improved, and meanwhile, the DNA repair effect and the anti-inflammatory effect are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a composition for staying up late and revitalizing the skin containing multiple plant extracts. Background Art

[0002] In today's fast-paced life, frequently staying up late has become the norm for many people. The resulting skin problems such as dullness, barrier damage, oxidative stress, accumulation of inflammatory factors and collagen metabolism disorders have given rise to a huge demand in the "staying up late to rejuvenate" skin care market.

[0003] Chinese patent CN110496090A discloses a composition and its use in preparing a skin care product for repairing skin damaged by staying up late. The invention provides a composition comprising adenosine, Leucophyllum aestivum bulb extract, and sodium hyaluronate. The combination of hyaluronic acid and adenosine achieves skin hydration and moisturizing. Small molecule hyaluronic acid can penetrate into the dermis of the skin to promote the proliferation of fibroblasts; medium molecule hyaluronic acid can penetrate into the stratum corneum to quickly replenish the moisture of the stratum corneum; large molecule hyaluronic acid stays on the surface of the skin to form a thin film that can effectively prevent the loss of moisture from the skin; adenosine, as a small molecule component, can quickly penetrate into the skin. In addition to being directly converted into energy for direct use by cells, it can also promote cell proliferation. After the skin barrier is repaired, the moisture content is increased, which can effectively avoid excessive secretion of physiological oil; Leucophyllum aestivum bulb extract has a strong cell repair effect, strengthens the skin's natural barrier, slows the proliferation of melanocytes, inhibits the production of melanin, and prevents the metabolic waste generated after staying up late from generating inflammation-induced melanin activation, resulting in the accumulation of large amounts of melanin in keratinocytes, causing dull skin.

[0004] Chinese patent CN110448478A discloses a composition and its application in the preparation of skin care products for regulating skin biological rhythms. The composition provided by the present invention includes adenosine, a skin conditioner A containing glycoproteins and amino acids, and a skin conditioner B containing glutamine ethylimidazole. The active ingredients contained in the skin conditioner B can regulate the expression of biological clock genes, promote the repair and proliferation of keratinocytes, promote the synthesis of vitamin D, activate the conversion of vitamin D, and thus activate the barrier capacity of the skin; the skin conditioner A rich in glycoproteins, glutamic acid, valine, and threonine is used to promote sugar degradation and mitochondrial respiration, ensuring that the ATP of skin cells is at a high level all day long, and ensuring that there is sufficient energy supply when skin cells need to be repaired at night; additional adenosine is added to directly supplement the energy needed by the cells. From the three dimensions of biological clock gene regulation + stimulation of the cell's own energy + additional energy supplementation, synergistic enhancement is achieved to solve skin problems caused by biological rhythm problems. However, in addition to addressing the problem of decreased skin repair ability caused by staying up late, current related products need to specifically address problems such as skin oxidation, inflammation, and collagen metabolism disorders caused by staying up late. Summary of the Invention

[0005] Based on this, it is necessary to provide a revitalizing composition for staying up late containing multiple plant extracts to address the current situation where existing skin care products are insufficient to improve problems such as skin oxidation, inflammation and collagen metabolism disorders caused by staying up late.

[0006] A multiple plant extraction method, the invention name includes the following steps: A1. Supercritical CO2 extraction of Mirabilis jalapa seeds was performed under the conditions of a pressure of 25-30 MPa, a temperature of 40-50°C, and an entrainer of ethanol (3-5% v / v) to obtain an extract A containing methyl jasmonate as the target component; A2. Low-temperature enzymatic hydrolysis and ultrasonic extraction of Rhodiola rosea roots and Edelweiss callus tissue were performed using 0.3-0.7% (w / w) cellulase at a pH of 4.5-5.5, an ultrasonic power of 250-350 W, and a treatment time of 20-40 min to obtain Extract B containing salidroside and edelweiss acid. A3. Green coffee beans are subjected to subcritical water extraction at a temperature of 110-130° C., a pressure of 8-12 MPa, and a solvent ratio of water to ethanol = 6:4-8:2 to obtain an extract C containing chlorogenic acid as the target component.

[0007] A rejuvenating composition for staying up late containing multiple plant extracts, the rejuvenating composition for staying up late comprising the above-mentioned extract A, extract B and extract C. Specifically, the rejuvenating composition for staying up late comprises the following components by mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener and 0.5-1 part of preservative composition.

[0008] Preferably, the rejuvenating composition for staying up late includes the following components by mass: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0009] Preferably, the rejuvenating composition for staying up late includes the following components by mass: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0010] Preferably, the rejuvenating composition for staying up late includes the following components by mass: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

[0011] Preferably, the rejuvenating composition for staying up late includes the following components by mass: 5 parts of extract A, 12.5 parts of extract B, 2.25 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

[0012] Preferably, the antiseptic composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:1.

[0013] In some embodiments, the number of extract A incorporated may be 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8, or 5.

[0014] In some embodiments, the number of parts of extract B incorporated may be set to 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, or 15.

[0015] In some embodiments, the number of extract C incorporated may be set to 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8 or 4.

[0016] In some embodiments, the incorporation fraction of curcumin nanoliposomes can be set to 1.5, 1.7, 1.9, 2.1, 2.3, 2.5, 2.7, 2.9 or 3.

[0017] A method for preparing the above-mentioned revitalizing composition for staying up late containing multiple plant extracts, the method for preparing the revitalizing composition for staying up late comprising the following steps: B1. Preparation of curcumin nanoliposomes; B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40°C and then vacuum dry to obtain nanoliposomes with an activity retention rate greater than 90%; B3. Take a predetermined amount of extract B, add 3 parts of β-glucan and dissolve in sufficient water at 60°C to complete the preparation of the aqueous phase composition; B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, add 0.5 parts of tocopherol, and dissolve them in a sufficient amount of organic solvent at 50° C. to complete the preparation of the oil phase composition; B5. The aqueous phase composition in step B3 and the oil phase composition obtained in step B4 are homogenized twice at 8000 psi high pressure to form an O / W emulsion. After cooling, 2 parts of lactobacillus fermentation filtrate are added to adjust the microecology to obtain a revitalizing composition for staying up late.

[0018] Preferably, the above step B1 includes the following steps: B11. Turmeric rhizome powder was ultrasonically defatted with n-hexane (1:10 w / v) three times (30 min / time) to remove oil; B12, 70% ethanol, solid-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentrate to obtain crude curcumin extract; B13, column chromatography purification: silica gel column (200-300 mesh), eluent chloroform: methanol (9:1), to obtain high-purity curcumin; B14. Phospholipids and cholesterol were mixed at a ratio of phospholipids: cholesterol = 7:3 (w / w) to obtain a lipid fraction. Curcumin was mixed with the lipid fraction at a ratio of curcumin: total lipids = 1:10. An appropriate amount of chloroform-methanol (2:1 v / v) was then added to dissolve the mixture, and vortexed until transparent. B15. Remove the organic solvent by rotary evaporation under reduced pressure at 40°C to form a uniform lipid film in the flask; B16. Add 46°C ultrapure water containing 0.5% Tween 80 to the plasma membrane flask in step B15, and shake in a 46°C constant temperature water bath for 30 minutes to form multilamellar liposomes. B17. High-pressure homogenize the multilamellar liposomes at a pressure of 15,000-18,000 psi for 3-5 times while controlling the temperature at 4-10° C. throughout the process to obtain curcumin nanoliposomes with a particle size of less than 100 nm.

[0019] The aforementioned revitalizing composition for staying up late, containing multiple plant extracts, is formulated with extract A containing methyl jasmonate as the target ingredient, extract B containing salidroside and edelweiss acid as the target ingredients, and curcumin nanoliposomes. Compared to similar products currently on the market, it can specifically enhance the antioxidant and anti-glycation capabilities of the revitalizing composition for staying up late, improve transdermal efficiency, and enhance DNA repair and anti-inflammatory effects. Specifically: (1) In the system of the present invention, based on the antioxidant and permeation-promoting effects of methyl jasmonate and the anti-inflammatory and free radical scavenging effects of curcumin nanoliposomes, methyl jasmonate can cooperate with curcumin to activate the Nrf2 pathway to enhance endogenous antioxidant capacity while directly neutralizing free radicals, thereby forming a double defense.

[0020] (2) Based on the anti-glycation and mitochondrial protection effects of salidroside and the lipid peroxidation inhibition and microcirculation promotion effects of chlorogenic acid, salidroside and chlorogenic acid can inhibit RAGE and reduce glycation stress, while downregulating LOX / COX and reducing inflammation, thereby jointly amplifying the antioxidant and anti-inflammatory effects of the late-night rejuvenation composition.

[0021] (3) Edelweiss acid promotes collagen synthesis through TGF-β, and curcumin inhibits the expression of MMP-1 / 9 and reduces collagen degradation, thereby achieving a balanced regulation of collagen synthesis and degradation; based on the permeation-promoting effect of methyl jasmonate, liposomes deliver curcumin to the dermis through the hair follicle bypass, thereby achieving a transdermal-retention synergistic effect of curcumin. DETAILED DESCRIPTION

[0022] To make the above-mentioned objects, features, and advantages of the present invention more readily apparent, specific embodiments of the present invention are described in detail below. The following description sets forth numerous specific details to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways than those described herein, and those skilled in the art may make similar modifications without departing from the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0023] The present invention discloses a multiple plant extraction method, which comprises the following steps: A1. Supercritical CO2 extraction of Mirabilis jalapa seeds was performed under the conditions of a pressure of 25-30 MPa, a temperature of 40-50°C, and an entrainer of ethanol (3-5% v / v) to obtain an extract A containing methyl jasmonate as the target component; A2. Low-temperature enzymatic hydrolysis and ultrasonic extraction of Rhodiola rosea roots and Edelweiss callus tissue were performed using 0.3-0.7% (w / w) cellulase at a pH of 4.5-5.5, an ultrasonic power of 250-350 W, and a treatment time of 20-40 min to obtain Extract B containing salidroside and edelweiss acid. A3. Green coffee beans are subjected to subcritical water extraction at a temperature of 110-130° C., a pressure of 8-12 MPa, and a solvent ratio of water to ethanol = 6:4-8:2 to obtain an extract C containing chlorogenic acid as the target component.

[0024] A rejuvenating composition for staying up late containing multiple plant extracts, the rejuvenating composition for staying up late comprising the above-mentioned extract A, extract B and extract C. Specifically, the rejuvenating composition for staying up late comprises the following components by mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener and 0.5-1 part of preservative composition.

[0025] Preferably, the rejuvenating composition for staying up late contains the above-mentioned extract A, extract B and extract C. Specifically, the rejuvenating composition for staying up late includes the following components by mass: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0026] Preferably, the rejuvenating composition for staying up late contains the above-mentioned extract A, extract B and extract C. Specifically, the rejuvenating composition for staying up late includes the following components by mass: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

[0027] Preferably, the antiseptic composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:1.

[0028] In some embodiments, the number of extract A incorporated may be 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8, or 5.

[0029] In some embodiments, the number of parts of extract B incorporated may be set to 10, 10.5, 11, 11.5, 12, 12.5, 13, 13.5, 14, 14.5, or 15.

[0030] In some embodiments, the number of extract C incorporated may be set to 2, 2.2, 2.4, 2.6, 2.8, 3, 3.2, 3.4, 3.6, 3.8 or 4.

[0031] In some embodiments, the incorporation fraction of curcumin nanoliposomes can be set to 1.5, 1.7, 1.9, 2.1, 2.3, 2.5, 2.7, 2.9 or 3.

[0032] A method for preparing the above-mentioned revitalizing composition for staying up late containing multiple plant extracts, the method for preparing the revitalizing composition for staying up late comprising the following steps: B1. Preparation of curcumin nanoliposomes; B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40°C and then vacuum dry to obtain nanoliposomes with an activity retention rate greater than 90%; B3. Take a predetermined amount of extract B, add 3 parts of β-glucan and dissolve in sufficient water at 60°C to complete the preparation of the aqueous phase composition; B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, add 0.5 parts of tocopherol, and dissolve them in a sufficient amount of organic solvent at 50° C. to complete the preparation of the oil phase composition; B5. The aqueous phase composition in step B3 and the oil phase composition obtained in step B4 are homogenized twice at 8000 psi high pressure to form an O / W emulsion. After cooling, 2 parts of lactobacillus fermentation filtrate are added to adjust the microecology to obtain a revitalizing composition for staying up late.

[0033] Preferably, the above step B1 includes the following steps: B11. Turmeric rhizome powder was ultrasonically defatted with n-hexane (1:10 w / v) three times (30 min / time) to remove oil; B12, 70% ethanol, solid-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentrate to obtain crude curcumin extract; B13, column chromatography purification: silica gel column (200-300 mesh), eluent chloroform: methanol (9:1), to obtain high-purity curcumin; B14. Phospholipids and cholesterol were mixed at a ratio of phospholipids: cholesterol = 7:3 (w / w) to obtain a lipid fraction. Curcumin was mixed with the lipid fraction at a ratio of curcumin: total lipids = 1:10. An appropriate amount of chloroform-methanol (2:1 v / v) was then added to dissolve the mixture, and vortexed until transparent. B15. Remove the organic solvent by rotary evaporation under reduced pressure at 40°C to form a uniform lipid film in the flask; B16. Add 46°C ultrapure water containing 0.5% Tween 80 to the plasma membrane flask in step B15, and shake in a 46°C constant temperature water bath for 30 minutes to form multilamellar liposomes. B17. High-pressure homogenize the multilamellar liposomes at a pressure of 15,000-18,000 psi for 3-5 times while controlling the temperature at 4-10° C. throughout the process to obtain curcumin nanoliposomes with a particle size of less than 100 nm.

[0034] In summary, the staying-up-night revitalizing composition containing multiple plant extracts of the present invention is able to specifically enhance the antioxidant and anti-glycation capabilities of the staying-up-night revitalizing composition, improve transdermal efficiency, and improve DNA repair and anti-inflammatory effects compared to similar products currently on the market by compounding extract A whose target ingredient is methyl jasmonate, extract B whose target ingredients are salidroside and edelweiss acid, and curcumin nanoliposomes. Specifically, in the system of the present invention, based on the antioxidant and penetration-promoting effects of methyl jasmonate and the anti-inflammatory and free radical scavenging effects of curcumin nanoliposomes, methyl jasmonate synergizes with curcumin to activate the Nrf2 pathway while enhancing endogenous antioxidant capacity and directly neutralizing free radicals, thereby forming a double defense; based on the anti-glycation and mitochondrial protective effects of salidroside and the lipid peroxidation and microcirculation rent inhibitory effects of chlorogenic acid, the salidroside system chlorogenic acid can inhibit RAGE to reduce glycation stress, while downregulating LOX / COX to reduce inflammation, thereby jointly amplifying the antioxidant and anti-inflammatory effects of the staying-up-night revitalizing composition. In addition, edelweiss acid promotes collagen synthesis through TGF-β, and curcumin inhibits MMP-1 / 9 expression and reduces collagen degradation, thereby achieving balanced regulation of collagen synthesis-degradation; based on the permeation-promoting effect of methyl jasmonate, liposomes deliver curcumin to the dermis through the hair follicle bypass, thereby achieving the transdermal-retention synergistic effect of curcumin.

[0035] The embodiments of the present invention will be described in detail below with reference to the examples. It should be understood that these examples are intended to illustrate the present invention only and are not intended to limit the scope of the invention. For experimental methods in the following examples where specific conditions are not specified, reference is made to the guidance provided in the present invention, and may also be made according to experimental manuals or conventional conditions in the art, or according to conditions recommended by the manufacturer, or with reference to experimental methods known in the art. Example 1

[0036] In this embodiment, the revitalizing composition for staying up late includes the following components by weight: 3 parts of extract A, 10 parts of extract B, 3 parts of extract C, 1.5 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnetite, 0.2 parts of a xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. The revitalizing composition for staying up late is obtained based on the above-mentioned multiple plant extraction method and the method for preparing the revitalizing composition for staying up late containing multiple plant extracts. Example 2

[0037] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the incorporation amount of extract B was set to 12.5 parts; and the incorporation amount of curcumin nanoliposomes was set to 2.25 parts. Example 3

[0038] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the number of extract B incorporated was set to 15; the number of curcumin nanoliposomes incorporated was set to 3. Example 4

[0039] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the incorporation amount of extract A was set to 4; and the incorporation amount of curcumin nanoliposomes was set to 2.25. Example 5

[0040] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the incorporation amount of extract A was set to 4; the incorporation amount of extract B was set to 12.5; and the incorporation amount of curcumin nanoliposomes was set to 3. Example 6

[0041] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the number of extract A incorporated was set to 4; the number of extract B incorporated was set to 15. Example 7

[0042] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the number of extract A incorporated was set to 5; the number of curcumin nanoliposomes incorporated was set to 3. Example 8

[0043] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the number of extract A incorporated was set to 5; and the number of extract B incorporated was set to 12.5. Example 9

[0044] A rejuvenating composition for staying up late was prepared according to the method of Example 1, except that the incorporation amount of extract A was set to 5; the incorporation amount of extract B was set to 15; and the incorporation amount of curcumin nanoliposomes was set to 2.25.

[0045] The revitalizing compositions prepared in Examples 1-9 were tested for ORAC values ​​using the fluorescence decay method, NO inhibition rate using the Griess method, transdermal permeation using the Franz diffusion cell method, and chlorogenic acid retention using HPLC. The test indicators included antioxidant capacity (ORAC value (μmol TE / g), anti-inflammatory effect (NO inhibition rate (%)), transdermal efficiency (24-hour cumulative permeation of chlorogenic acid (μg / cm²), and stability (high-temperature retention rate of chlorogenic acid (%)). The results are shown in Table 1.

[0046]

[0047] Performing range analysis on the data in the above table yields the following: (1) Curcumin nanoliposomes are the core variable: they have the greatest impact on transdermal efficiency and anti-inflammatory effects.

[0048] (2) Extract A significantly improves antioxidant activity: the addition of 5 servings increases the ORAC value by nearly 40%.

[0049] (3) Extract B needs to be added in sufficient amounts: 15 parts has the best anti-glycation and DNA repair effects.

[0050] In summary, the theoretically optimal incorporation ratio of Extract A, Extract B, and Curcumin nanoliposomes in the multi-plant extract revitalizing composition of the present invention is: 5 parts Extract A, 15 parts Extract B, and 3 parts Curcumin nanoliposomes. Based on the orthogonal test, its performance is predicted to be: ORAC value: 1400-1500 μmol TE / g, NO inhibition rate: 85-88%, transdermal permeation: 14-15 μg / cm², and chlorogenic acid retention rate: 90-92%. In actual production, for cost control purposes, the incorporation ratios of Extract B and Curcumin nanoliposomes can be balanced to achieve a balanced incorporation ratio of: 5 parts Extract A, 12.5 parts Extract B, and 2.25 parts Curcumin nanoliposomes, thereby reducing raw material costs by approximately 15%.

[0051] In order to verify the performance of the above-mentioned optimal incorporation amount and balanced incorporation amount compared with the currently available products, further comparative verification tests of the theoretical optimal group, balanced group and control group will be designed below to verify the actual performance of the optimal solution and balanced solution of the present invention.

[0052] Control Example A commercially available overnight essence containing 3% niacinamide + 1% caffeine was used to test ORAC, NO inhibition rate and transdermal efficiency.

[0053] Theoretical optimal example In this example, the revitalizing composition for staying up late includes the following components by weight: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnetite, 0.2 parts of a xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. Based on the above-mentioned multiple plant extraction method and preparation method for a revitalizing composition for staying up late containing multiple plant extracts, a revitalizing composition for staying up late was obtained, and ORAC, NO inhibition rate, and transdermal efficiency testing were performed.

[0054] Balance Example In this example, the revitalizing composition for staying up late includes the following components by weight: 5 parts of extract A, 12.5 parts of extract B, 3 parts of extract C, 2.25 parts of curcumin nanoliposomes, 3 parts of Lactobacillus fermentation filtrate, 2 parts of water-locking magnetite, 0.2 parts of a xanthan gum / acrylate thickener, and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin. Based on the above-mentioned multiple plant extraction method and preparation method for a revitalizing composition for staying up late containing multiple plant extracts, a revitalizing composition for staying up late was obtained, and ORAC, NO inhibition rate, and transdermal efficiency testing were performed.

[0055] Then, the verification data of the control example, the theoretical optimal example and the balanced example were compared, and the results are shown in Table 2 below.

[0056]

[0057] In summary, the rejuvenating composition for staying up late containing multiple plant extracts of the present invention has the best comprehensive performance when its components are combined as the theoretical optimal example compared with similar products currently on the market; and in actual production and sales, it can be adjusted according to actual production costs, such as the above-mentioned balanced example, whose comprehensive performance is second only to the theoretical optimal example, but better than the control example.

[0058] The technical features of the above-mentioned embodiments can be combined arbitrarily. In order to make the description concise, not all possible combinations of the technical features in the above-mentioned embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0059] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A multiple plant extraction method, characterized in that: The steps include: A1. Supercritical CO2 extraction of Mirabilis jalapa seeds was performed under the conditions of a pressure of 25-30 MPa, a temperature of 40-50°C, and an entrainer of ethanol (3-5% v / v) to obtain an extract A containing methyl jasmonate as the target component; A2. Low-temperature enzymatic hydrolysis and ultrasonic extraction of Rhodiola rosea roots and Edelweiss callus tissue were performed using 0.3-0.7% (w / w) cellulase at a pH of 4.5-5.5, an ultrasonic power of 250-350 W, and a treatment time of 20-40 min to obtain Extract B containing salidroside and edelweiss acid. A3. Green coffee beans are subjected to subcritical water extraction at a temperature of 110-130° C., a pressure of 8-12 MPa, and a solvent ratio of water to ethanol = 6:4-8:2 to obtain an extract C containing chlorogenic acid as the target component.

2. A composition for staying up late and revitalizing containing multiple plant extracts, characterized in that: The composition for staying up late and revitalizing the body comprises the extract A, extract B and extract C described in claim 1.

3. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The rejuvenating composition for staying up late includes the following components by mass: 3-5 parts of extract A, 10-15 parts of extract B, 2-4 parts of extract C, 1.5-3 parts of curcumin nanoliposomes, 2-5 parts of lactobacillus fermentation filtrate, 1-3 parts of water-locking magnet, 0.1-0.3 parts of xanthan gum / acrylate compound thickener and 0.5-1 parts of preservative composition.

4. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The rejuvenating composition for staying up late includes the following components by mass: 3-4 parts of extract A, 10-12.5 parts of extract B, 3 parts of extract C, 1.5-2.25 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

5. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The rejuvenating composition for staying up late includes the following components by mass: 4-5 parts of extract A, 12.5-15 parts of extract B, 3 parts of extract C, 2.25-3 parts of curcumin nanoliposomes, 4 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of and 0.8 parts of preservative composition.

6. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The rejuvenating composition for staying up late includes the following components by mass: 5 parts of extract A, 15 parts of extract B, 3 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

7. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The rejuvenating composition for staying up late includes the following components by mass: 5 parts of extract A, 12.5 parts of extract B, 2.25 parts of extract C, 3 parts of curcumin nanoliposomes, 3 parts of lactobacillus fermentation filtrate, 2 parts of water-locking magnet, 0.2 parts of xanthan gum / acrylate compound thickener and 0.8 parts of a 3:1 mixture of phenoxyethanol and ethylhexylglycerin.

8. The composition for staying up late and revitalizing containing multiple plant extracts according to claim 2, characterized in that: The antiseptic composition is a mixture of phenoxyethanol and ethylhexylglycerin in a mass ratio of 3:

1.

9. A method for preparing the revitalizing composition for staying up late containing multiple plant extracts according to any one of claims 2 to 8, characterized in that: The preparation method of the staying-up-late-night revitalizing composition comprises the following steps: B1. Preparation of curcumin nanoliposomes; B2. Add 3% w / w trehalose and 2% w / w mannitol to the curcumin nanoliposomes obtained in step B1, pre-freeze at -40°C and then vacuum dry to obtain nanoliposomes with an activity retention rate greater than 90%; B3. Take a predetermined amount of extract B, add 3 parts of β-glucan and dissolve in sufficient water at 60°C to complete the preparation of the aqueous phase composition; B4. Take a predetermined amount of extract A and the nanoliposomes obtained in step B2, add 0.5 parts of tocopherol, and dissolve them in a sufficient amount of organic solvent at 50° C. to complete the preparation of the oil phase composition; B5. The aqueous phase composition in step B3 and the oil phase composition obtained in step B4 are homogenized twice at 8000 psi high pressure to form an O / W emulsion. After cooling, 2 parts of lactobacillus fermentation filtrate are added to adjust the microecology to obtain a revitalizing composition for staying up late.

10. The method for preparing the composition for staying up late and revitalizing the body containing multiple plant extracts according to claim 9, characterized in that: The above-mentioned step B1 includes the following steps: B11. Turmeric rhizome powder was ultrasonically defatted with n-hexane (1:10 w / v) three times (30 min / time) to remove oil; B12, 70% ethanol, solid-liquid ratio 1:15, reflux extraction at 50℃ for 2 hours, and concentrate to obtain crude curcumin extract; B13, column chromatography purification: silica gel column (200-300 mesh), eluent chloroform: methanol (9:1), to obtain high-purity curcumin; B14. Phospholipids and cholesterol were mixed at a ratio of phospholipids: cholesterol = 7:3 (w / w) to obtain a lipid fraction. Curcumin was mixed with the lipid fraction at a ratio of curcumin: total lipids = 1:

10. An appropriate amount of chloroform-methanol (2:1 v / v) was then added to dissolve the mixture, and vortexed until transparent. B15. Remove the organic solvent by rotary evaporation under reduced pressure at 40°C to form a uniform lipid film in the flask; B16. Add 46°C ultrapure water containing 0.5% Tween 80 to the plasma membrane flask in step B15, and shake in a 46°C constant temperature water bath for 30 minutes to form multilamellar liposomes. B17. High-pressure homogenize the multilamellar liposomes at a pressure of 15,000-18,000 psi for 3-5 times while controlling the temperature at 4-10° C. throughout the process to obtain curcumin nanoliposomes with a particle size of less than 100 nm.

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