Nutrition therapy-based functional potion capable of benefiting qi and nourishing blood and preparation process of nutritional therapy-based functional potion

Abstract

The invention discloses a nutritional therapy-based functional potion for benefiting qi and nourishing blood and a preparation process thereof, and belongs to the technical field of functional foods.The preparation process comprises the following steps: preparing beta-cyclodextrin, ergothioneine and an iron source into an inclusion compound; the inclusion compound, the colloid, the red ginseng, the red date concentrated juice, the lemon concentrated juice and the like are added into a reaction container to form a synergistic effect mechanism of tonifying qi, generating blood and promoting absorption, and the traditional Chinese medicine composition has a more remarkable hematopoietic function improvement effect compared with a single iron agent or a traditional blood replenishing traditional Chinese medicine; the ergothioneine can remarkably promote absorption of iron, and the iron source and the ergothioneine are prepared into the inclusion compound, so that the stability of the ergothioneine and the iron is improved, the damage of gastric acid is avoided, and the bioavailability of the potion is improved; through synergistic compatibility of the active component of red ginseng and iron, the multiplication capacity of bone marrow stroma cells can be remarkably enhanced, and the qi and blood biochemical function of the body is improved; ginsenoside contained in the red ginseng has the effect of promoting hematopoiesis and can activate proliferation and differentiation of hematopoietic stem cells.

Description

Technical Field

[0001] This application belongs to the field of functional food technology, and in particular relates to a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy and its preparation process. Background Technology

[0002] With the accelerating pace of society, high-intensity work, staying up late, and irregular eating habits are leading to increasingly prominent problems of qi and blood deficiency. According to the "China Sub-health Population Survey Report," 76% of office workers experience symptoms of qi and blood deficiency such as fatigue and low immunity; 60% of adults suffer from poor blood circulation due to prolonged sitting and lack of exercise; and 45% of urban white-collar workers experience anemia and pale complexion due to long-term sleep deprivation. Meanwhile, both men and women are experiencing different levels of sub-health symptoms. Men are prone to qi deficiency (lack of energy and fatigue) due to high work pressure, excessive alcohol consumption, and high-fat diets, and the prevalence of anemia in men has reached 12% ("Report on Nutrition and Chronic Diseases of Chinese Residents"). Women, affected by menstruation, pregnancy, and menopause, have an iron-deficiency anemia incidence rate as high as 30%, and long-term qi and blood deficiency can easily lead to problems such as dull skin, hair loss, and menstrual irregularities. With the expansion of the sub-healthy population and the increasing demand for precision nutrition, developing a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy, scientific formulation, and gender differentiation has broad market prospects and can fill existing market gaps.

[0003] Most iron supplements on the market currently use vitamin C to promote iron absorption. However, vitamin C is easily oxidized, which requires high-quality products and is prone to degradation. Ergothioneine, as a novel iron absorption promoter, can inhibit the expression of hepcidin, thus relieving its inhibitory effect on iron absorption. Cell experiments have shown that it can significantly improve the iron absorption rate of Caco-2 cells. However, ergothioneine has poor stability, high water solubility, and is easily degraded in the acidic gastric environment. Summary of the Invention

[0004] The purpose of this application is to provide a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy and its preparation process, which improves the sub-health state caused by qi and blood deficiency through the compounding of medicinal and food homologous ingredients, thereby solving the technical problems of poor stability of ergothioneine and poor bioavailability of iron supplements in the prior art.

[0005] To achieve the above objectives, the technical solution adopted in this application is: to provide a preparation process for a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy, specifically including the following steps: (i) Iron source encapsulation: β-cyclodextrin was mixed with water and heated to obtain a β-cyclodextrin solution; ergothioneine was dissolved in water to obtain an ergothioneine solution; the β-cyclodextrin solution, ergothioneine solution and iron source were mixed and ultrasonicated, and then dried to obtain an inclusion complex; (ii) Mix the adhesive, solid sugar alcohol and water and homogenize to obtain a colloid; (III) Add inclusion complex, colloid, donkey-hide gelatin peptide, red ginseng powder, red date concentrate, concentrated lemon juice, red date concentrate, red ginseng extract, and purified water to a reaction vessel, mix and stir, filter, bottle, sterilize, and package to obtain a qi-tonifying and blood-nourishing beverage.

[0006] In one embodiment, The iron source is one of ferrous sulfate, ferrous gluconate, polysaccharide iron complex, or compound ferrous sulfate and folic acid.

[0007] In one embodiment, The adhesive is one of xanthan gum, cellulose gum, or locust bean gum.

[0008] In one embodiment, By weight, β-cyclodextrin: 115-163 parts, ergothioneine: 14.4-20.4 parts, iron source: 12-17 parts, gelling agent: 3-5 parts, solid sugar alcohol: 15-30 parts, donkey-hide gelatin peptide: 0.1-0.3 parts, red ginseng powder: 0.25-0.5 parts, jujube concentrate: 5.5-7.5 parts, concentrated lemon juice: 0.22-0.27 parts, jujube concentrate: 0.23-0.5 parts, red ginseng extract: 7.5-7.9 parts, purified water: 300-350 parts; The recommended addition amount of jujube concentrate is 460-470 mg / bag, and the recommended addition amount of lemon concentrate is to adjust the pH to 3.8-4.3. Jujubes are rich in iron and cyclic adenosine monophosphate (cAMP), which can work synergistically with red ginseng and iron supplements to enhance the blood-replenishing effect. The organic acids and vitamin C in lemon concentrate can promote iron absorption and stabilize iron ions, preventing oxidation. The amount of red ginseng powder added is 5 mg / bag. Its ultra-fine pulverized form can improve the dissolution rate and bioavailability of ginsenosides, and further enhance the activation effect on bone marrow stromal cells. Red ginseng extract is prepared by low-temperature extraction process to retain the heat-sensitive active ingredients in red ginseng (such as ginsenosides, polysaccharides, etc.) and ensure its hematopoietic regulation effect. In one embodiment, Step (1): The mass ratio of β-cyclodextrin to water is 1:6, and the mass ratio of ergothioneine to water is 1:2.

[0009] In one embodiment, The cavity diameter of β-cyclodextrin is 6.0-6.5 Å.

[0010] In one embodiment, Step (1) The heating temperature is 50-60 ℃, the ultrasonic power is 300-500 W, and the time is 20-30 min; the drying operation conditions are: pre-freezing at -50 ℃ and vacuum drying at -30 ℃ for 24 h.

[0011] In one embodiment, The specific steps for homogenization in step (II) are as follows: dry mix the solid sugar alcohol and the adhesive until the color is uniform, put them into the feeding machine, dry mix and circulate for 5 minutes, then start homogenization at 65% power and 65℃ for 15 minutes.

[0012] In one embodiment, Step (3) requires mixing for 15 minutes.

[0013] This application also provides a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy, which is prepared by the preparation process of any of the above embodiments.

[0014] This application provides a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy and its preparation process. The composition of this application, through the compounding of red ginseng (tonifying qi and generating blood), iron source (direct iron supplementation), red date concentrate (nourishing blood and calming the mind), and ergothioneine and lemon concentrate (promoting iron absorption), forms a synergistic mechanism of "tonifying qi → generating blood → promoting absorption," which has a more significant effect on improving hematopoietic function than single iron supplements or traditional blood-tonifying Chinese medicines. This beverage, through the synergistic combination of active components of red ginseng and ferrous gluconate, can significantly enhance the proliferation capacity of bone marrow stromal cells (BMSCs) and inhibit their apoptosis, thereby optimizing the hematopoietic induction microenvironment (HIM) and improving the body's qi and blood biochemical function. The ginsenosides (such as Rg1 and Rb1) contained in red ginseng have a hematopoietic-promoting effect and can activate the proliferation and differentiation of hematopoietic stem cells. Ferrous gluconate, as a biologically active ingredient, can also promote hematopoiesis. This highly bioavailable iron source directly replenishes the key elements required for hemoglobin synthesis. The synergistic effect of these two components significantly improves the bone marrow microenvironment and promotes hematopoiesis. Ergothioneine significantly promotes iron absorption. Preparing the iron source and ergothioneine into an inclusion complex enhances the stability of both, preventing damage from gastric acid and improving the bioavailability of the beverage. HIM is the core internal environment for maintaining normal hematopoiesis. Its function depends on growth factors and extracellular matrix secreted by bone marrow stromal cells, thereby regulating the proliferation, differentiation, and survival of hematopoietic cells. Through precise formulation, it maximizes the activity of BMSCs, enhances hematopoietic function, and leaves the user with abundant blood and energy. This composition conforms to the concept of "medicine and food sharing the same origin," inheriting the traditional wisdom of blood-tonifying and qi-boosting dietary therapy while combining modern nutrition science to achieve safe and effective daily conditioning effects. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this application, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 HE staining image of experimental group in Example 2; Figure 2 HE staining image of the control group in Experiment 2. Detailed Implementation

[0017] To make the technical problems, technical solutions, and beneficial effects to be solved by this application clearer, this application will be further described in detail. It should be understood that the specific embodiments described herein are only for explaining this application and are not intended to limit this application.

[0018] Example 1 A preparation process for a qi-tonifying and blood-nourishing medicinal beverage based on nutritional therapy specifically includes the following steps: Raw materials weighed: by weight, β-cyclodextrin: 163 parts, ergothioneine: 20.4 parts, ferrous sulfate (calculated as iron): 17 parts, xanthan gum: 5 parts, solid sugar alcohol: 30 parts, donkey-hide gelatin peptide: 0.3 parts, red ginseng powder: 0.5 parts, jujube concentrate: 7.5 parts, concentrated lemon juice: 0.27 parts, jujube concentrate: 0.5 parts, red ginseng extract: 7.9 parts, purified water: 350 parts; (I) Iron source encapsulation: 163 parts of β-cyclodextrin with a cavity diameter of 6.0 Å were mixed with 978 parts of water and heated to 60℃ to dissolve, thus obtaining a β-cyclodextrin solution; 20.4 parts of ergothioneine were dissolved in 40.8 parts of water to obtain an ergothioneine solution; the β-cyclodextrin solution, ergothioneine solution and ferrous sulfate were mixed, sonicated at 400 W for 25 min, pre-frozen at -50 ℃, and vacuum dried at -30 ℃ for 24 h to obtain the inclusion complex; the encapsulation rate of ergothioneine was 92.6% and the encapsulation rate of ferrous sulfate was 96.3% as detected by UV.

[0019] (ii) Dry mix solid sugar alcohol and xanthan gum until the color is uniform, put them into the feeder, dry mix and circulate for 5 minutes, then add water to start homogenization, power 65%, keep at 65 ℃ for 15 minutes to homogenize and obtain colloid. (III) Add inclusion complex, colloid, donkey-hide gelatin peptide, red ginseng powder, red date concentrate, concentrated lemon juice, red date concentrate, red ginseng extract, and purified water to a reaction vessel, mix and stir for 15 min, filter, can, sterilize, and package to obtain a qi-tonifying and blood-nourishing beverage.

[0020] Example 2 The difference between this embodiment and Example 1 is that ferrous sulfate is replaced with ferrous gluconate, while the rest of the operations are the same, and the inclusion complex is obtained; the encapsulation rate of ergothioneine by ultraviolet detection is 93.7%, and the encapsulation rate of ferrous gluconate is 95.5%.

[0021] Example 3 The difference between this embodiment and Example 1 is that ferrous sulfate was replaced with a polysaccharide-iron complex, while the rest of the operations were the same, and the inclusion complex was obtained. The encapsulation rate of ergothioneine was 93.2% as detected by ultraviolet light, and the encapsulation rate of the polysaccharide-iron complex was 97%.

[0022] Example 4 The difference between this embodiment and Example 1 is that ferrous sulfate was replaced with compound ferrous sulfate folic acid, while the rest of the operations were the same, and the inclusion complex was obtained; the encapsulation rate of ergothioneine was 92.8% and the encapsulation rate of compound ferrous sulfate folic acid was 96.2% as detected by ultraviolet light.

[0023] Example 5 The difference between this embodiment and Example 1 is that, by weight, the following components are used: β-cyclodextrin: 115 parts, ergothioneine: 14.4 parts, iron source: 12 parts, gelling agent: 3 parts, solid sugar alcohol: 15 parts, donkey-hide gelatin peptide: 0.1 parts, red ginseng powder: 0.25 parts, jujube concentrate: 5.5 parts, concentrated lemon juice: 0.22 parts, jujube concentrate: 0.23 parts, red ginseng extract: 7.5 parts, purified water: 300 parts. The remaining operations are the same.

[0024] Example 6 The difference between this embodiment and Example 1 is that, by weight, the following components are used: β-cyclodextrin: 139 parts, ergothioneine: 17.4 parts, iron source: 14.5 parts, gelatin: 4 parts, solid sugar alcohol: 22.5 parts, donkey-hide gelatin peptide: 0.2 parts, red ginseng powder: 0.38 parts, jujube concentrate: 6.5 parts, concentrated lemon juice: 0.25 parts, jujube concentrate: 0.35 parts, red ginseng extract: 7.7 parts, purified water: 325 parts. The remaining operations are the same.

[0025] Example 7 The difference between this embodiment and Example 1 is that the cavity diameter of β-cyclodextrin is 6.5 Å, while the other operations are the same, and the inclusion complex is obtained; the encapsulation rate of ergothioneine is 89.7% and the encapsulation rate of ferrous sulfate is 92.1% as detected by ultraviolet light.

[0026] Example 8 The difference between this embodiment and embodiment 1 is that the heating temperature in step (i) is 50 ℃, while the rest of the operations are the same.

[0027] Example 9 The difference between this embodiment and embodiment 1 is that the ultrasonic power in step (1) is 300 W and the time is 20 min. The rest of the operation is the same, and the inclusion complex is obtained. The ultraviolet detection shows that the encapsulation rate of ergothionein is 87.4% and the encapsulation rate of ferrous sulfate is 90.3%.

[0028] Example 10 The difference between this embodiment and embodiment 1 is that the ultrasonic power in step (1) is 500 W and the time is 30 min. The rest of the operation is the same, and the inclusion complex is obtained. The ultraviolet detection shows that the encapsulation rate of ergothionein is 93.2% and the encapsulation rate of ferrous sulfate is 96.5%.

[0029] Example 11 The difference between this embodiment and Embodiment 1 is that xanthan gum is replaced with cellulose gum; the rest of the operations are the same.

[0030] Example 12 The difference between this embodiment and Embodiment 1 is that xanthan gum is replaced with locust bean gum; the rest of the operations are the same.

[0031] Experimental Example 1 Example 1 was subjected to a gastric acid environment stability test. The experimental steps are as follows: 1. Solution preparation Simulated gastric acid solution (SGF): Prepare an HCl solution with pH=1.2 using ultrapure water, and add pepsin to achieve a final concentration of approximately 0.1-0.3% (w / v). Prepare immediately before use. Iron stock solution: Prepare a FeSO4 solution (10 mM) of a certain concentration using ultrapure water that has been boiled and deoxygenated. Ergothioneine stock solution: Prepare ergothioneine solution (10mM) with ultrapure water. Termination / Development Buffer: Prepare a buffer containing sufficient amounts of HEPES (pH 7.0) and phenanthridine; 2. Grouping and Incubation The following experimental groups were set up (each group had 3 replicates): Experimental group: Inclusion complex prepared in Example 1 + SGF; Control group: SGF, FeSO4 solution, and ergothioneine solution were added to the reaction tube. 2+ The final concentration was the same as that of ergothioneine, which was 0.5 mM. Blank group: Only SGF; All solutions were immediately placed in a 37 ℃ constant temperature water bath shaker and oscillated at a slow speed to simulate gastric peristalsis. 3. Sampling and Detection At preset time points (0 minutes, 30 minutes, 60 minutes, 120 minutes), accurately aspirate equal amounts of sample from each reaction tube; immediately add the aspirated sample to a test tube containing HEPES buffer to terminate the reaction; Color development: Add phenanthroxazine colorimetric solution to a test tube, mix thoroughly, and let stand at room temperature for 10-30 minutes to allow Fe to develop color. 2+ React fully with phenanthridine; Absorbance determination: Using a spectrophotometer, with the blank group zeroed, the absorbance value of each test tube was measured at a wavelength of 562 nm; HPLC was used to test the change in ergothioneine concentration. 4. Results: The absorbance of the control group decreased rapidly over time, indicating that a large amount of Fe was present in the acidic environment of the stomach. 2+ It was oxidized to Fe 3+ (Fe) 3+ It does not react with phenanthroxazine and does not develop a color. Experimental group: The rate of decrease in absorbance was significantly slower than that of the control group. Fe was measured at various time points. 2+ The remaining amounts were significantly higher than those in the control group; HPLC testing showed that the peak height of ergothionein in the control group was significantly lower than that in the experimental group. The above results indicate that the inclusion complex can effectively slow down the oxidative degradation of ergothioneine and iron ions in the gastric acid environment, avoid the destruction by gastric acid, and improve the bioavailability of iron source.

[0032] Experimental Example 2 The supplement prepared in Example 1 was used to incubate BMSCs cells for 48 h as the experimental group, while blank BMSCs cells were incubated for 48 h as the control group. Cell production was observed by HE staining; the results are as follows. Figure 1-2 As shown in the two HE staining images, it can be seen that the BMSCs cells supplemented with the supplements, the active components of red ginseng and ferrous gluconate can significantly enhance the proliferation capacity of bone marrow stromal cells (BMSCs), while the BMSCs cells in the control group grow slowly and have more apoptotic cells.

[0033] This application provides a qi-tonifying and blood-nourishing functional beverage based on nutritional therapy and its preparation process, including the following steps: mixing β-cyclodextrin with water and heating to obtain a β-cyclodextrin solution; dissolving ergothioneine in water to obtain an ergothioneine solution; mixing the β-cyclodextrin solution, ergothioneine solution, and an iron source, followed by ultrasonication and drying to obtain an inclusion complex; homogenizing a gelling agent, solid sugar alcohol, and water to obtain a colloid; adding the inclusion complex, colloid, donkey-hide gelatin peptide, red ginseng powder, jujube concentrate, concentrated lemon juice, jujube concentrate, red ginseng extract, and purified water to a reaction vessel, mixing and stirring, filtering, bottling, sterilizing, and packaging to obtain the qi-tonifying and blood-nourishing functional beverage; Based on the concept of food and medicine sharing the same origin and the essence of ancient formulas for replenishing both qi and blood, this application provides a dietary therapy composition with the effects of strengthening the spleen and generating blood, and nourishing qi and blood. This composition is scientifically formulated with authentic medicinal materials, iron absorption promoters and natural iron supplements, in line with the traditional Chinese medicine principles of nourishing like with like and regulating the organs with flavor. It can be consumed daily to improve qi and blood deficiency and improve the bioavailability of iron. This application solves the problems of difficult swallowing, slow absorption and poor component stability of traditional solid preparations. Moreover, this functional beverage does not require decoction, brewing or chewing, and can be drunk directly after opening the bag. The scientifically formulated liquid form enables rapid penetration and absorption of nutrient molecules, making replenishing qi and blood as simple and efficient as drinking water. The above description is merely a preferred embodiment of this application and is not intended to limit this application. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A preparation process of a functional drink for tonifying qi and nourishing blood based on nutrition therapy, characterized in that, Specifically comprising the following steps: (I) iron source embedding: β-cyclodextrin is mixed with water and heated to prepare a β-cyclodextrin solution; ergothioneine is dissolved in water to prepare an ergothioneine solution; the β-cyclodextrin solution, the ergothioneine solution and an iron source are mixed and then subjected to ultrasonic treatment, and a clathrate is prepared after drying; (II) homogenizing the gum, solid sugar alcohol and water to prepare a colloid; (III) adding the clathrate, the colloid, donkey-hide gelatin peptide, red ginseng powder, red date concentrate, concentrated lemon juice, red date concentrate juice, red ginseng extract, purified water into a reaction container, mixing and stirring, filtering, canning, sterilizing and packaging to prepare a functional drink for tonifying qi and nourishing blood.

2. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The iron source is one of ferrous sulfate, ferrous gluconate, polysaccharide iron complex or compound ferrous sulfate folic acid.

3. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The gum is one of xanthan gum, cellulose gum or locust bean gum.

4. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, According to weight parts, β-cyclodextrin: 115-163 parts, ergothioneine: 14.4-20.4 parts, iron source: 12-17 parts, gum: 3-5 parts, solid sugar alcohol: 15-30 parts, donkey-hide gelatin peptide: 0.1-0.3 parts, red ginseng powder: 0.25-0.5 parts, red date concentrate: 5.5-7.5 parts, concentrated lemon juice: 0.22-0.27 parts, red date concentrate juice: 0.23-0.5 parts, red ginseng extract: 7.5-7.9 parts, and purified water: 300-350 parts.

5. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The mass ratio of β-cyclodextrin to water in step (I) is 1:6, and the mass ratio of ergothioneine to water is 1:

2.

6. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The cavity diameter of the β-cyclodextrin is 6.0-6.5 Å.

7. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The heating temperature in step (I) is 50-60°C, the ultrasonic power is 300-500 W, and the time is 20-30 min; the operation conditions for drying are pre-freezing at -50°C and vacuum drying at -30°C for 24 h.

8. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The specific steps for homogenization in step (II) are as follows: dry mixing the solid sugar alcohol and the gum until the color is uniform, feeding into a feeding machine, and then homogenizing after 5 min of dry mixing cycle at a power of 65% and a temperature of 65°C for 15 min.

9. The preparation process of the functional drink for tonifying Qi and nourishing blood based on nutrition therapy according to claim 1, characterized in that, The mixing and stirring time in step (III) is 15 min.

10. A functional drink for tonifying Qi and nourishing blood based on nutrition therapy, characterized in that, Prepared by the preparation process of any one of claims 1-9.

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