A device and method for detecting the binding process of odorant binding protein and pheromone

Abstract

The invention discloses an apparatus and a method for detecting a combination process of odorant-binding protein and pheromone. The apparatus comprises a sample introduction system, a measure transition system, a counter electrode, a reference electrode, a working electrode, a measure groove, an oscillator, a liquid-outlet peristaltic pump, a waste liquid groove and a computer; the sample introduction system consists of a first peristaltic pump, a second peristaltic pump, a third peristaltic pump, a first injector, a second injector, a third injector and a controller; and the measure transition system consists of a measure circuit, a high-resistance electrometer and a measure circuit. The method comprises preparing honeybee pheromone isoamyl-acetate standard solutions with different concentration, an apis-cerana-ceraca odorant-binding protein (Acer-ASP2) standard solution, and mixed solutions of apis-cerana-ceraca odorant-binding protein (Acer-ASP2) and honeybee pheromone isoamyl acetate and with different concentration, and detecting variation of electrochemical impedance of the three kinds of solutions along with time, so as to obtain corresponding electrochemical impedance spectrograms, and further to obtain the combination of odorant-binding protein and pheromone.

Description

technical field [0001] The invention relates to an analysis method based on electrochemical impedance spectroscopy, in particular to a device and method for detecting the binding process of odorant binding protein and pheromone. Background technique [0002] In the field of biosensing, electrochemical impedance detection technology is widely used because of its low cost, easy signal processing and analysis, and real-time detection. Odor-binding protein is an extracellular low-molecular-weight hydrophobic protein that is easy to purify and can specifically bind to target molecules. In previous studies, the research on odorant-binding proteins mainly used fluorescent labeling and mass spectrometry. Fluorescent labeling requires labeling of the target, while mass spectrometry requires very expensive equipment. Utilizing the advantages of odorant-binding protein and electrochemical impedance detection technology, a method based on electrochemical impedance spectroscopy was deve...

AI Technical Summary

Problems solved by technology

In previous studies, the research on odorant-binding proteins mainly used fluorescent labeling and mass spectrometry. Fluorescent labeling requires labeling of the target, while mass spectrometry requires very expensive equipment.

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