Preparing method and application of odorant binding protein sensor based on impedance analysis

An odor-binding protein and impedance analysis technology, applied in the direction of electrochemical variables of materials, can solve problems such as complex operation, and achieve the effects of high detection sensitivity, low detection limit and strong specificity

Active Publication Date: 2013-12-25
ZHEJIANG UNIV
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Problems solved by technology

[0002] At present, the detection of chemical substances such as floral fragrance substances and bee pheromones is mainly through mass spectrometry, fluorescence and other methods, but the equipment is sophisticated and the operation is complicated

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  • Preparing method and application of odorant binding protein sensor based on impedance analysis
  • Preparing method and application of odorant binding protein sensor based on impedance analysis
  • Preparing method and application of odorant binding protein sensor based on impedance analysis

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0018] The preparation method of the odor binding protein sensor based on electrochemical impedance analysis of the present invention comprises the following steps:

[0019] 1. Processing of interdigital gold electrode impedance sensor.

[0020] The processing of interdigitated gold electrodes adopts standard processing technology. Using glass with a thickness of 0.5 mm and a diameter of 4 inches as the substrate, a 20 nm thick titanium tungsten (TiW) film was magnetron sputtered on the glass as an adhesion layer, followed by a 300 nm thick gold (Au ) thin film as the electrode layer; after the electrode pattern is etched with AZ photoresist, the non-electrode area is etched away by dry etching to obtain the interdigitated gold electrode 1, as figure 1 shown. Finally, the chip cavity 3 made of plex...

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Abstract

The invention discloses a preparing method and application of an odorant binding protein sensor based on impedance analysis. A plane interdigital gold electrode array is formed by machining in a standard MEMS technology, a nitrocellulose membrane is used for processing the surface of an interdigital gold electrode, odorant binding protein is directly fixed, and the impedance sensor is obtained. An analyzing method with impedance and molecular docking combined is used, and the Chinese bee odorant binding protein Acer-ASP2 is fixed on the interdigital gold electrode and is used for detecting flower fragrance matter and bee pheromone. The established electrochemical sensor can fix the odorant binding protein on the surface of the interdigital gold electrode stably and easily, sensor detecting sensitivity is high, detecting lower limit is low, and specificity is strong.

Description

technical field [0001] The present invention relates to a preparation technology of a sensing device, in particular to a preparation method of an electrochemical impedance sensor for detecting floral aroma substances and honeybee pheromones by using Chinese honeybee odorant binding protein (Acer-ASP2), and the combination of impedance sensing technology and molecular docking technology Combined Applied Analysis Methods. Background technique [0002] At present, the detection of chemical substances such as floral fragrance substances and bee pheromones is mainly through mass spectrometry, fluorescence and other methods, but the instruments and equipment are sophisticated and the operation is complicated. In the field of biosensing, electrochemical impedance detection technology is widely used in the detection of cell and protein-specific responses due to its low cost, real-time, non-destructive, high throughput, and easy display and processing of signals. Odor-binding protei...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 刘清君卢妍利王平张迪鸣张倩
Owner ZHEJIANG UNIV
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