Coding gene OBP11 for OBPs (odorant binding proteins) of brown planthopper and application

A brown planthopper and brown planthopper-resistant technology is applied in the fields of application, genetic engineering, plant gene improvement, etc., to achieve the effect of reducing the amount of pesticides and manpower input

Inactive Publication Date: 2019-02-15
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Before the present invention was published, there was no publi

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The coding gene OBP11 of a brown planthopper odor binding protein is obtained by the following method:

[0022] Using the brown planthopper cDNA as a template, design primers O1: 5'-ATGAAGTCGTTCATCGTTTG-3'; O2: -TCACGGCCGTCCTCCTTCTTA-3' for PCR amplification. The PCR conditions are as follows:

[0023] 3min, 95℃; 30s 94℃; and 60s 57℃, 1min 72℃35 cycles; 10min, 72℃; 1min, 25℃.

[0024] Finally, the OBP11 gene was obtained, its sequence is shown in SEQ ID NO.1, and the corresponding amino acid sequence is shown in SEQ ID NO.2.

Embodiment 2

[0026] The application of OBP11 gene in the control of brown planthopper to breed new resistant rice varieties is as follows:

[0027] Using the rice 35S promoter and DS1301 as the interference vector, construct a recombinant vector capable of producing double-stranded RNA hairpin structure, and transform the constructed recombinant vector into Agrobacterium tumefaciens strain (EHA105). The PCR primers during the vector construction process are as follows : Upstream primer: 5'-CCGGAATTCATGAAGTCGTTC-3'; Downstream primer: -CCGCTCGAGTCACGGCCGTC-3', using the commonly used Agrobacterium-mediated genetic transformation method in this field, the embryogenic callus induced by Japonica rice Zhonghua 11 seeds was used as the recipient Soak and infect the callus with the Agrobacterium suspension medium containing the final carrier of the target gene, and select positive plants on the selection medium containing hygromycin. The specific method refers to the highly efficient genetic tran...

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Abstract

The invention belongs to the technical field of biology and particularly discloses a coding gene OBP11 for OBPs (odorant binding proteins) of brown planthopper and an application. The amino acid sequence of the OBPs of the brown planthopper is shown as SEQ ID NO.2. The OBPs play an essential role in growth and development processes of the brown planthopper, a lethal effect can be realized on the brown planthopper by silencing the gene, the sequence has stronger target specificity, and the killing effect on other types of insects and species is avoided. Therefore, a new direction and theoretical basis are provided for control of the brown planthopper and culture of resistant varieties.

Description

technical field [0001] The invention belongs to the field of biotechnology, and mainly relates to a brown planthopper OBP protein coding gene OBP11 and its application. Background technique [0002] The brown planthopper is a common and important rice field pest in my country. It is a kind of special-feeding pest on rice and wild rice widely distributed in Asia. It starts from Hainan in the south of my country and reaches Jilin in the north, mainly in the Yangtze River Basin, Southwest China, South China and other rice planting areas. , because it is a piercing-sucking pest with strong reproductive ability and is mainly distributed at the base of rice bushes, making it difficult to control. With the intensification of the use of chemical fertilizers and pesticides, the resistance of brown planthoppers has increased, resulting in an increase in the occurrence of brown planthoppers year by year, seriously affecting the yield of rice in my country. In order to explore new metho...

Claims

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Application Information

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IPC IPC(8): C07K14/435C12N15/12C12N15/82A01H5/00A01H6/46
CPCC07K14/43563C12N15/8218C12N15/8286
Inventor 王满囷刘豪
Owner HUAZHONG AGRI UNIV
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