Method for detecting self-mutilation rate of insects by using odorant binding protein 15 gene

An odor-binding protein and insect technology, which is applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of increasing self-mutilation of the same kind, time-consuming, labor-intensive and costly, and decreased ability of insect odor perception, etc., to achieve wide source of materials , easy to purchase, and simple to operate

Inactive Publication Date: 2014-02-05
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

However, the detection method for the self-mutilation rate of commercial stinkbugs from different food sources or different merchants is still to test the self-mutilation rate during the breeding process, which is time-consuming, laborious and expensive
It is difficult to quickly test goods without biological assays
[0005] Odorant binding protein 15 (odorant binding protein 15) is a protein for insects to sense external odors. Changes in the expression level of odorant binding protein 15 will affect the degree to which insects perceive external odors. The decrease in the expression level of odorant binding protein 15 gene will make insects more sensitive to similar odors. Decreased perception, which can greatly increase the phenomenon of self-mutilation of the same kind

Method used

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  • Method for detecting self-mutilation rate of insects by using odorant binding protein 15 gene
  • Method for detecting self-mutilation rate of insects by using odorant binding protein 15 gene
  • Method for detecting self-mutilation rate of insects by using odorant binding protein 15 gene

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Embodiment 1

[0043] Example 1. Discovery of Odor Binding Protein 15 and its Encoding Gene for Detecting Insect Self-mutilation Rate and Design of Special Primers

[0044] Studies have found that the odorant binding protein 15 gene is a good molecular marker for detecting the self-mutilation rate of insects, so the present invention uses the expression of the odorant binding protein 15 (odorant binding protein 15) encoding gene of the stinkbug to detect the self-mutilation rate of the stinkbug .

[0045] The nucleotide sequence of the gene encoding the odor binding protein 15 of the gnat bug is sequence 1 in the sequence listing or sequence 1 in the sequence listing from the 10th to 273rd nucleotides at the 5' end, and the amino acid sequence of the protein is in the sequence listing Sequence 2 of .

[0046] The primers designed to amplify the coding gene according to the coding gene of odorant binding protein 15 of the stinkbug are as follows:

[0047] Upstream primer: ATTACCGACATTAGGAGA...

Embodiment 2

[0049] Example 2. Application of Odor Binding Protein 15 or Encoding Gene or Special Primer in Detecting the Self-mutilation Rate of C. stinkbug

[0050] The cockroach (Arma chinensis) adopted in the following experiments is recorded in Taxonomic and bionomic notes on Arma chinensis (Fallou) (Hemiptera: Pentatomidae: Asopinae, Zootaxa, 3382: 41-52, 2012, the public can obtain from Chinese Academy of Agricultural Sciences Plant Protection Research Obtained) were divided into two groups according to the difference of food quality, 50 in each group:

[0051] Cockbugs eating artificial diet: Cockbugs fed artificial diet (27±1°C, 16:8(L:D), 75±5%RH) were fed with 160 μl of artificial diet per adult per day until the Stinkbugs die naturally;

[0052] The prey-feeding group of gnats: the gnats that feed on prey (27±1°C, 16:8(L:D), 75±5%RH) each pair of adults feeds one prey, every 7-15 days according to When the prey is eaten, the prey is replaced once until the stinkbug dies natur...

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Abstract

The invention discloses a method for detecting the self-mutilation rate of insects by using an odorant binding protein 15 gene. The invention provides an application of a substance for detecting the expression level of an odorant binding protein 15 coding gene in insect populations fed with different substances in detection of the self-mutilation rate of the insect populations fed with different substances; and the amino acid sequence of an odorant binding protein 15 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the self-mutilation rate of the insects, in particular to the self-mutilation rate of arma chinensis with different nutrient sources; and the detection of the self-mutilation rate of the insects by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for detecting self-mutilation rate of insects by using odor binding protein 15 gene. Background technique [0002] Biological pest control plays an irreplaceable and important role in the sustainable development of agriculture and forestry. The mass production of natural enemy insects is the most basic and important link in biological control. The predatory natural enemy insect, Arma chinensis, can control a variety of agricultural and forestry pests from Lepidoptera, Coleoptera, Hemiptera, Homoptera, Hymenoptera, etc., especially it can also control the major invasive pests potato beetle and The American white moth, therefore, is a natural enemy insect commodity with good application prospects. [0003] As a commodity, reducing the cost of production is a way for people to pursue profit maximization. In the production of the natural enemy insect, the stink bug, different...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/12C07K14/435
Inventor 邹德玉陈红印张礼生王树英陈长风王孟卿刘晨曦
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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