Method for detecting self-mutilation rate of insects by using odorant binding protein 15 gene

An odor-binding protein and insect technology, which is applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of increasing self-mutilation of the same kind, time-consuming, labor-intensive and costly, and decreased ability of insect odor perception, etc., to achieve wide source of materials , easy to purchase, and simple to operate
CN103060467BInactive Publication Date: 2014-02-05INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2014-02-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for detecting the self-mutilation rate of insects by using an odorant binding protein 15 gene. The invention provides an application of a substance for detecting the expression level of an odorant binding protein 15 coding gene in insect populations fed with different substances in detection of the self-mutilation rate of the insect populations fed with different substances; and the amino acid sequence of an odorant binding protein 15 is sequence 2 in a sequence table. According to the method disclosed by the invention, experiments prove that the detection method provided by the invention can be used for detecting the self-mutilation rate of the insects, in particular to the self-mutilation rate of arma chinensis with different nutrient sources; and the detection of the self-mutilation rate of the insects by the detection method disclosed by the invention only requires 2-3 days. However, the use of a traditional biological method requires about 30-60 days. The detection method disclosed by the invention has the advantages of wide range of involved material sources, easiness in purchase, simplicity in operation, and time-saving and labor-saving properties, and is suitable for popularization and application in commodity inspection and detection of insects.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a method for detecting self-mutilation rate of insects by using odor binding protein 15 gene. Background technique

[0002] Biological pest control plays an irreplaceable and important role in the sustainable development of agriculture and forestry. The mass production of natural enemy insects is the most basic and important link in biological control. The predatory natural enemy insect, Arma chinensis, can control a variety of agricultural and forestry pests from Lepidoptera, Coleoptera, Hemiptera, Homoptera, Hymenoptera, etc., especially it can also control the major invasive pests potato beetle and The American white moth, therefore, is a natural enemy insect commodity with good application prospects.

[0003] As a commodity, reducing the cost of production is a way for people to pursue profit maximization. In the production of the natural enemy insect, the stink bug, different...

Claims

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