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Preparation method of nanocup array biosensor device for ionone detection

A sensing device and ionone technology, which is applied in the field of preparation of nanocup array biosensors, can solve problems such as hindering applications, and achieve the effects of high specificity, simple and convenient operation, and avoiding complicated optical paths.

Active Publication Date: 2015-08-12
ZHEJIANG UNIV
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Problems solved by technology

However, its generally required complex optical system design hinders further applications

Method used

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  • Preparation method of nanocup array biosensor device for ionone detection
  • Preparation method of nanocup array biosensor device for ionone detection
  • Preparation method of nanocup array biosensor device for ionone detection

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Embodiment Construction

[0017] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but the present invention is not limited.

[0018] The preparation method of the nanocup array biosensor device used for ionone detection of the present invention comprises the following steps:

[0019] 1. Processing of nanocup array structure of sensor devices. The sensor nanocup structures were fabricated using standard nanoimprinting techniques. The sensor is based on a square PET (polyethylene terephthalate) material with a thickness of 250 μm and a length of 2 cm. First, UV-curable adhesive is evenly coated on the PET material as an adhesive layer, and then regular tapered nano-pillars are used to The array template imprints the nanocup geometric structure array on the adhesive layer; and then uses the DYMAX EC series UV irradiation system at room temperature at 105mW / cm 2 Power density irradiation for 60s makes the nanocup array sensor ...

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Abstract

The invention discloses a preparation method of a nano cup array biosensor for ionone detection. The method comprises the steps that a nanoscale cup-shaped structure is constructed by a nano impressing technology; gold nanoparticles are deposited on a side wall of a nano cup; odorant binding protein Acer-ASP2 is fixed on the side wall of the nano cup by electrostatic adsorption of the gold nanoparticles; during detection, a target molecule ionone is specifically bound with the odorant binding protein to result in change of an optical refraction characteristic of the side wall of the nano cup; and a spectrum of transmission light passing through the biosensor is changed correspondingly, so that the detection of the target molecule ionone is realized. Compared with the traditional ionone detection means, the nano cup array biosensor avoids a complicated light path required by optical detection and is simple, convenient and quick to operate based on the advantages of high sensitivity and specificity of the optical detection means.

Description

technical field [0001] The invention relates to a preparation technology of a biosensor device, in particular to a preparation method of a nanocup array biosensor device used for detection of edible essence ionone. Background technique [0002] Ionone is a food flavor widely used in the food industry. For its detection, traditional physical and chemical methods such as absorbance detection and titration are generally used, which usually have the problems of complicated operation and low detection efficiency. Odor-binding protein Acer-ASP2, as the olfactory receptor of bees, can specifically bind to specific target molecules, and can be used as a biological sensitive element to construct a biosensor. Optical biosensors are mainly a means of detecting optical changes caused by specific reactions between biological components (proteins, cells, and tissues) and target molecules. Due to their advantages of high sensitivity, no labeling, and short analysis time, etc. It is the k...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/01G01N21/25
Inventor 刘清君张迪鸣王平卢妍利张倩
Owner ZHEJIANG UNIV
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