Botanical attractant for sternum theobratum as well as preparation method and application of botanical attractant

Abstract

The invention discloses a botanical attractant for thymus fasciatus as well as a preparation method and application of the botanical attractant. The botanical attractant comprises hexyl acetate, nonanal, capraldehyde and beta-ocimene. The preparation method of the attractant specifically comprises the following step: uniformly mixing hexyl acetate, nonanal, capraldehyde and beta-ocimene to obtain the attractant. The four compounds are all volatile components generated by induction when the thymus theophyllus eats oil tea leaves, and each compound has an attraction effect on thymus theophyllus adults when being independently used, has a synergistic effect when being compounded for use, has a more remarkable attraction effect on thymus theophyllus, and can be used as an attractant for thymus theophyllus. The compound can be added into a conventional trap or used in cooperation with an environment-friendly red and yellow plate to be used for green prevention and control of the thoracic leaflet beetles. In addition, the botanical attractant for the sternum theobratum is low in cost, harmless to people and livestock, environmentally friendly and not prone to generating drug resistance.

Description

Technical Field

[0001] The present invention relates to the technical field of green pest control, and more specifically to a plant-based attractant for tea leaf beetles, and a preparation method and application thereof. Background Art

[0002] Basilepta melanopus, commonly known as the black-legged horned leaf beetle, belongs to the family Chrysomelidae in the order Coleoptera. This insect poses a serious threat to oil-tea tea and tea trees.

[0003] The adult of this insect is light brown to brown, with a body length of 3.2-5.8mm. The female insect is 0.9-1.4mm larger than the male insect. The female adult has an inverted "eight"-shaped black spot at the end of the last abdominal segment, while the male insect does not have this feature. In Hunan, the tea horn-breasted leaf beetle has one generation per year. The larvae lay eggs and hibernate in the topsoil and fallen leaves. The adults begin to emerge in April when the temperature is above 20℃ for 6 days and the soil moisture is about 79%. The adults are active from May to June, and the number gradually decreases after July.

[0004] The damage of the tea leaf beetle lasts for a long time and covers a wide area. Its larvae feed on the fibrous roots of tea oil plants. After emerging the following year, the adults climb to the treetops to feed on the backs of the newly sprouted tender shoots and leaves. The irregular round holes left by the gnaws connect into pieces, causing the leaves to be riddled with holes and wilt or fall off prematurely. The roots and new leaves of the tea oil plants are seriously damaged, which harms the growth and fruiting of the tea oil plants. The insect has a strong reproductive capacity. Adults can lay eggs many times in their lifetime, with a maximum daily egg production of more than 200 eggs, and an average of 275.5 eggs per female, which causes it to quickly form a disaster under suitable conditions, increasing the difficulty of prevention and control. Current prevention and control measures mainly include chemical control, manual control and biological control, among which chemical control is particularly commonly used.

[0005] The tea leaf beetle has now developed into a major pest in the oil tea industry. For large-scale prevention and control, aerial control methods using various pesticides alone or in combination are adopted. Although the pesticides themselves have a good killing effect, when they are actually used in the forest, they will encounter difficulties in reaching the pests in time. Because the adult insects have weak flying ability when they just emerge from the soil, they will crawl up from the stems of the oil tea trees and eat the lower tender leaves within a week after emerging from the unearthed. The trees in the oil tea forest are often densely spaced, and it is difficult to spray the pesticides on the lower leaves during aerial control. Therefore, it is easy to miss the prevention and control, resulting in the phenomenon that the tender leaves appear to be less damaged on the surface, but the lower leaves are severely damaged. Although manual control can effectively avoid the above problems, it is time-consuming and labor-intensive in actual operation, which is not conducive to large-scale prevention and control. In addition, although there is a patent for using yellow boards for attraction and chemical agents for control, the yellow boards are not the specific tropism color of leaf beetles, and some natural enemies may also be lured and killed. Moreover, the capture rate of tea-horned leaf beetles under natural conditions is low, generally less than 20 heads, which is far from achieving the purpose of luring and killing a large number of leaf beetles.

[0006] Physical and chemical attractant control technology is an important technical measure for green control of tea leaf beetles. It is mainly achieved by hanging color plates and attractant information compounds in the field. Plant volatiles are a type of information chemical released by plants. Insects mainly perceive external volatile information chemicals through their sense of smell and make corresponding behavioral responses. Plant-derived volatile information compounds can change the behavior of insects and play an important role in the insects' search for food, mates, and hosts. These volatile information substances can be used to attract, repel, and gather insects.

[0007] In summary, relying on chemical pesticides to control tea leaf beetles has obvious limitations. In order to respond to the country's call for "reducing weight and reducing medication", finding a green, safe and effective method to control tea leaf beetles is a difficult problem that technical personnel in this field urgently need to solve. Summary of the invention

[0008] In view of this, the object of the present invention is to provide a plant-based attractant for tea leaf beetles and a preparation method and application thereof, which can not only effectively control the damage caused by tea leaf beetles, is harmless to humans and animals, is environmentally friendly, and is not prone to drug resistance, but also can avoid pesticide pollution and food safety problems caused by chemical control, so as to solve the shortcomings of the prior art.

[0009] In order to achieve the above object, the present invention adopts the following technical solution:

[0010] A plant-derived attractant for tea horn-breasted leaf beetle, comprising hexyl acetate, nonanal, decanal and β-ocimene.

[0011] The present invention is to solve the problem that the control of tea horn chest leaf beetle in the prior art is still mainly based on chemical pesticide control, which easily leads to a series of problems such as pest resistance, environmental pollution, and destruction of forest ecosystems. A plant-based attractant for tea horn chest leaf beetle is provided, specifically hexyl acetate, nonanal, decanal and β-ocimene with attractant activity. These four compounds are volatile components induced when tea horn chest leaf beetle takes oil tea leaves, and each compound has an attracting effect on tea horn chest leaf beetle adults when used alone, and has a synergistic effect when used in combination, and has a more significant attracting effect on tea horn chest leaf beetle. It can be added to a conventional trap or used in conjunction with an environmentally friendly red and yellow board for green prevention and control of tea horn chest leaf beetle. Moreover, the plant-based attractant for tea horn chest leaf beetle of the present invention has low cost, is harmless to humans and animals, is environmentally friendly, and is not easy to produce drug resistance.

[0012] Furthermore, the mass ratio of hexyl acetate, nonanal, decanal and β-ocimene is 2:1:1:1.

[0013] A method for preparing the above-mentioned plant-derived attractant for tea horn-breasted leaf beetles specifically comprises the following steps: uniformly mixing hexyl acetate, nonanal, decanal and β-ocimene to obtain the plant-derived attractant for tea horn-breasted leaf beetles.

[0014] The present invention also seeks to protect the use of the plant-derived attractant for tea horn-breasted leaf beetles or the plant-derived attractant for tea horn-breasted leaf beetles prepared by the above-mentioned preparation method in preventing and controlling tea horn-breasted leaf beetles.

[0015] Furthermore, the above application specifically includes the following steps: adding the plant-derived attractant of the tea leaf beetle to a conventional trap or using it in conjunction with a yellow-red environmentally friendly insect trap board.

[0016] It can be seen from the above technical solution that compared with the prior art, the beneficial effects of the present invention are as follows:

[0017] 1. The present invention first uses solid phase microextraction (SPME) to collect plant volatiles produced during the feeding process of tea leaf beetles on young leaves of oil tea, then uses gas chromatography-mass spectrometry (GC-MS) to analyze the compound components of the collected mixed compounds, combines gas chromatography-antennenburg potential (GC-EAD) to identify and analyze the compounds with electrophysiological reactions in the volatiles, purchases standard products to identify active compounds and further uses an olfactory behavior tester to evaluate the behavioral responses of tea leaf beetles to different active compounds, determines the active compounds used in oil tea forests based on the results, combines environmentally friendly red and yellow insect attractant boards to evaluate the trapping and killing effects of different compounds on tea leaf beetles, and prepares a compound agent to evaluate its luring effect on tea leaf beetles, thereby providing a theoretical and practical application basis for green prevention and control and population monitoring of tea leaf beetles.

[0018] 2. When using the environmentally friendly insect trap for tea green leafhoppers disclosed in application number 201820607868.1, the applicant found that it also had a certain trapping effect on tea leaf beetles. By combining plant volatiles, the trapping amount of tea leaf beetles can be doubled. After combining the insect trap with plant volatiles and hanging it on the lower stems of the oil tea tree, it can effectively trap the tea leaf beetles in the early stage of their emergence and reduce the amount of insects by more than 70%.

[0019] 3. The present invention can accurately trap and kill the tea leaf beetle by hanging an insect trap board with plant-derived attractants before the tea leaf beetle emerges, which can not only effectively avoid the use of chemical agents, but also effectively control the total amount of pests before major damage occurs. At the same time, one insect trap board can be used for half a month. According to the maximum period of the tea leaf beetle adult, it is two months, and it only needs to be replaced three times in the middle. 2Hang a board randomly, and only 18 boards are needed for one acre of land. First, it is low-cost, easy to hang, good trapping effect, and long-lasting effect; second, it is environmentally friendly and safe, and can effectively control the insect population without chemical agents; third, it has high-efficiency attractant activity, using a highly specific plant-based attractant to specifically improve the attractant activity to tea horn chest beetles, and using environmentally friendly insect traps can protect natural enemies. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The olfactory selection behavior of the tea leaf beetle on the young tea leaves and whether the tea leaf beetle adults were added; Note: ANOVA test, ** and *** indicate that the attraction of the tea leaf beetle to the tea leaf beetle when the young tea leaves were added to the tea leaf beetle was significantly different from the control (air) at the P<0.01 and P<0.001 levels respectively;

[0021] Figure 2 This is the total ion mass spectrum of volatiles in young tea leaves fed by the tea leaf beetle; Note: "1" represents hexyl acetate, "2" represents β-ocimene, "3" represents nonanal, and "4" represents decanal;

[0022] Figure 3 This is a comparison of five replicate total ion chromatograms of volatiles produced when young tea leaves were fed by tea leaf beetles.

[0023] Figure 4 The electrophysiological responses of four different volatiles to the tea horn chest beetle; Note: A is the EAG relative response value of different concentrations of hexyl acetate, B is the EAG relative response value of different concentrations of nonanal, C is the EAG relative response value of different concentrations of decanal, and D is the EAG relative response value of different concentrations of β-ocimene; Different lowercase letters indicate that there are significant differences between different concentrations of the same volatile according to Tukey analysis (P<0.05);

[0024] Figure 5 The selection behavior of four different volatiles on tea horn-breasted leaf beetles; Note: A is the selection rate of tea horn-breasted leaf beetles to different concentrations of hexyl acetate, B is the selection rate of tea horn-breasted leaf beetles to different concentrations of nonanal, C is the selection rate of tea horn-breasted leaf beetles to different concentrations of decanal, and D is the selection rate of tea horn-breasted leaf beetles to different concentrations of β-ocimene; *, **, *** indicate that the number of tea horn-breasted leaf beetles trapped by the same volatile and the same concentration is significantly different from the number of tea horn-breasted leaf beetles trapped by the control (n-hexane) at the P<0.05, P<0.01, and P<0.001 levels, respectively, after ANOVA test;

[0025] Figure 6 Comparison of the attracting effects of four compounds mixed in different mass ratios on tea leaf beetles in the forest; Note: The values ​​in the figure are mean ± standard deviation (n = 10), and different letters indicate significant differences among different concentrations by Tukey analysis (P < 0.05). DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] Example 1

[0028] The plant-based attractant for tea horned leaf beetle includes hexyl acetate, nonanal, decanal and β-ocimene in a mass ratio of 2:1:1:1.

[0029] The method for preparing the above-mentioned plant-derived attractant for tea horn-breasted leaf beetle specifically includes the following steps: uniformly mixing hexyl acetate, nonanal, decanal and β-ocimene in a mass ratio of 2:1:1:1 to obtain the plant-derived attractant for tea horn-breasted leaf beetle.

[0030] Performance Testing

[0031] 1. The trapping effect of plant-derived attractants

[0032] The experiment was conducted on May 5, 2024 in the camellia oleifera germplasm forest of the National Camellia Oil Engineering Center in Yuhua District, Changsha City, Hunan Province. No chemical pesticides are used in the camellia oleifera forest all year round, and the planted varieties are the Xianglin series.

[0033] Take the plant-derived attractant prepared in Example 1, dilute it to 100 μg / μL using n-hexane as solvent, add 250 μL to each empty rubber head attractant core to make a plant-derived attractant attractant core. Select three oil tea forests with tea horn-breasted leaf beetle control needs as three repeated test plots, with different plots separated by more than 15m, and randomly place the following devices in each plot for treatment:

[0034] (1) Experimental group: consisting of plant-derived attractant core and red and yellow sticky insect trap;

[0035] (2) Blank group: red and yellow sticky insect board without plant-derived attractant core.

[0036] The above device is placed at a height of 0.5-1m from the trunk of the tea tree, and every 25m 2 Place 1. The number of trapped insects on the sticky insect board was investigated every 24 h. The data of the forest trapping experiment were analyzed by one-way analysis of variance (ANOVA) (p<0.05). Tukey's multiple test (p<0.05) was used to compare the differences between the mean values ​​in pairs. The results are shown in Table 1.

[0037] Table 1 Trapping results of plant-derived attractants

[0038]

[0039] Note: *** indicates extremely significant difference (P < 0.001, Tukey method).

[0040] As shown in Table 1, the number of tea leaf beetles trapped by the plant-derived attractant in Example 1 was relatively large, which was significantly higher than that in the blank group (P < 0.001).

[0041] 2. The tropism behavior of tea leaf beetle adults towards tea oil tea leaves and whether tea leaf beetle adults are present

[0042] A Y-type olfactometer was used to test the tropism behavior of adult tea leaf beetles towards young leaves of oil-tea tea and leaves with added tea leaf beetles.

[0043] The Y-shaped olfactometer is composed of an air pump, a drying tower, a gas washing bottle, a odor source bottle, a flow meter, and a Y-shaped glass tube connected in sequence by Teflon tubes. The air flow rate of the two arms is controlled at 200 mL / min by a gas flow meter. The drying tower is filled with activated carbon for air purification, and the gas washing bottle is filled with 2 / 3 distilled water for humidification. All glassware is cleaned with anhydrous ethanol and then with distilled water. In order to avoid the interference of uneven ambient light intensity on the selection behavior of leaf beetles, a piece of black cloth is used to cover the entire Y-shaped glass tube so that the light intensity of the two test arms is the same. The experiment was carried out at a room temperature of 26°C and a relative humidity of 70%-75%, and the experiment was carried out at the time when the insects are more active (16:00 to 20:00).

[0044] Young tea leaves and tea leaf beetles were placed in odor source bottles, with air as a blank control. Each time, one adult tea leaf beetle was selected and placed in a Y-shaped glass tube for the experiment. Observation was performed within 5 minutes after the start of each experiment. When the leaf beetle entered 2 / 3 of the side wall within 5 minutes and stayed for more than 30 seconds, it was recorded as a response to the substance in the side wall. When the leaf beetle did not make a choice after entering the side wall for 5 minutes, the behavioral observation was terminated. 100 adult tea leaf beetles were tested in each treatment. In order to eliminate the influence of the tube wall position effect, the two side arm positions were exchanged for every 5 leaf beetles tested, and a clean Y-shaped glass tube was replaced for every 10 leaf beetles tested. After each test, the Teflon tube, washing bottle and Y-shaped glass tube were cleaned with anhydrous ethanol and dried before use to eliminate the odor influence between different treatments.

[0045] Figure 1 The results showed that compared with the control (air), the young tea leaves (healthy leaves) and the young tea leaves (infested leaves) infested with adult tea leaf beetles had a very significant attraction to tea leaf beetles (P<0.001); compared with the simple young tea leaves (healthy leaves), the young tea leaves (infested leaves) infested with adult tea leaf beetles attracted more adult tea leaf beetles, and the difference was significant.

[0046] 3. Collection and identification of volatile substances when the tea leaf beetle feeds on young tea leaves

[0047] In this experiment, dynamic headspace adsorption method and gas chromatography-mass spectrometry (GC-MS instrument, purchased from Changzhou Pannuo Instrument Co., Ltd., model: A91Plus GC-MS) were used to collect and identify the volatiles when the tea leaf beetle fed on the young leaves of oil tea. The freshly collected young leaves of oil tea were placed in a collection bag, and the tea leaf beetle was added under indoor conditions of temperature of 26°C and relative humidity of 60%-75%. The volatiles during the feeding process were collected by solid phase microextraction. The microextraction needle was SPME-PDMS / DVB, and each collection lasted for 6 to 10 hours.

[0048] Chromatographic conditions: Pannuo gas chromatography-mass spectrometer GC-MS, DB-1MS chromatographic column (30m×0.32mm×0.25μm), injection port temperature: 250℃, nitrogen as carrier gas, carrier gas flow rate 1mL / min, in 112Kpa constant pressure mode, no split injection. Mass spectrometry conditions: ion source EI 70eV, voltage 350V, interface temperature 230℃, scanning voltage range 33~400m / Z. Heating program: starting temperature is 50℃, rise to 180℃ at 10℃ / min, keep for 2min, then rise to 280℃ at 5℃ / min, keep for 5min. 1μL sample is injected manually each time. The total ion chromatogram obtained is used to calculate the content of each component by peak area normalization method, and compared with the NIST17 (National Institute of Standards and Technology) database to obtain the name, peak time, molecular formula, CAS number and relative content of each substance. The collection and identification of volatiles were repeated 5 times. The volatiles with the highest frequency and a matching degree of more than 90% in the 5 repetitions were determined to be the volatiles produced by the tea leaf beetle feeding on the young leaves of oil tea, and standard samples were purchased for verification. The standard was diluted to 1 ppm with n-hexane, and 10 μL of the standard dilution was taken using a 50 μL microinjector and the experiment was carried out according to the same GC-MS procedure. The types of volatiles were determined by matching the NIST database and standard identification. The results are shown in Table 2 and Figure 2 shown.

[0049] Table 2 Identification results of the main active components of volatiles from young leaves of tea oil tea fed by tea leaf beetles

[0050]

[0051]

[0052] There are 55 compounds in the volatiles of young tea leaves fed by the tea leaf beetle. After removing impurities and retaining substances with high matching degree, 12 volatiles remain ( Figure 2 , Table 2), among which hexyl acetate, nonanal, decanal, and β-ocimene were all stable in the five repetitions ( Figure 3 ).

[0053] 4. Electrophysiological responses of the tea leaf beetle to volatiles from its feeding on young tea leaves

[0054] Insect antennal potential (EAG) experiments, chemical standards were diluted with n-hexane, and the concentrations of dose-response chemical standards were 0.01μg / μL, 0.1μg / μL, 1μg / μL, 10μg / μL, and 100μg / μL. The reference electrode and recording electrode consisted of two glass capillaries (1.1mm in diameter) filled with Ringer's electrolyte and two silver wires, each of which was immersed in a glass capillary. The head was cut off at the groove between the leaf beetle head and the prothorax, and a small part of the two front legs and the ends of the two antennae was removed. The head was placed in the reference electrode and the two antennae were placed in the recording electrode for testing.

[0055] Filter paper (3mm×40mm) was placed in a 1mL pipette tip, and 15μL of the test compound at concentrations of 0.01μg / μL, 0.1μg / μL, 1μg / μL, 10μg / μL and 100μg / μL was added, respectively. The same volume of filter paper (3mm×40mm) treated with n-hexane was used as a control. The tip of the pipette tip was inserted into a stainless steel tube with a diameter of 12mm and a length of 200mm. The end was connected by a silicone tube. The air flow blown out of the stainless steel tube was about 5mm away from the test compound or n-hexane (control). A new pipette tip must be replaced before each experiment. The filter paper strip treated with n-hexane in the pipette tip was used as a control. The n-hexane blank control was tested once before (EAG CK1) and after (EAG CK2) the chemical standard test. The stimulus (test compound or n-hexane control) was blown into a continuous humidified air flow generated by the air stimulus controller for 0.5s. The recording of EAG signals starts 1 second before the onset of stimulation and lasts for 10 seconds. Between each stimulation, the tactile receptors (antennae) have at least 1 minute of recovery time before testing. The relative value of each compound reference can be obtained by subtracting the average of the two previous and subsequent controls from the absolute value of each compound reference. Each chemical standard was measured 10 times, with 10 beetles each time. By comparing the electrophysiological responses of the antennae of adult tea horn-breasted leaf beetles to volatiles and controls, volatiles that trigger antennae responses of adult tea horn-breasted leaf beetles were screened out. The results are shown in the figure. Figure 4 shown.

[0056] Figure 4 The results showed that the tea leaf beetle adults were sensitive to hexyl acetate ( Figure 4 A in), β-ocimene ( Figure 4 B in), nonanal ( Figure 4C) and decanal ( Figure 4 D) These four volatiles all have electrophysiological responses. Moreover, the EAG responses of adults of the tea horn-breasted leaf beetle to these four volatiles within a certain concentration range increase with the increase of concentration.

[0057] 5. The selection behavior of tea leaf beetle adults on volatiles from tea oil leaf feeding

[0058] The experiment set up the comparison of tea horn-breasted leaf beetle adults to four different volatiles (hexyl acetate, nonanal, decanal, β-ocimene) and blank control (air), the comparison between different concentrations of the same volatiles, and the comparison between the optimal concentrations of four different volatiles. The Y-type olfactometer test was used, and the experimental method was the same as the above-mentioned Y-type olfactometer test. The results are as follows Figure 5 shown.

[0059] Adult tea leaf beetles showed behavioral responses to the four volatiles. Among them, 1μg / μL, 10μg / μL and 100μg / μL of hexyl acetate and nonanal ( Figure 5 A, B), 10μg / μL and 100μg / μL decanal ( Figure 5 C) and β-ocimene ( Figure 5 D) in the figure all had a very significant attraction effect on the adults of the tea leaf beetle (P<0.001).

[0060] 6. Forest attraction test of four compounds mixed in different mass ratios against tea leaf beetles

[0061] The forest luring test was carried out in the oil tea forest. According to the volatiles that have an attracting effect on the tea horn-breasted leaf beetle screened by the Y-type olfactometer indoor, the volatiles were diluted with paraffin oil to the corresponding concentration for use. The four compounds had a very significant attracting effect at a concentration of 10μg / μL. Based on this, combined with cost and application practice, the four compounds were all selected at a concentration of 10μg / μL as the test concentration. The mixing mass ratios of the four compounds hexyl acetate, nonanal, decanal and β-ocimene were 1:1:1:1 (ratio 1), 2:1:1:1 (ratio 2), 1:2:1:1 (ratio 3), 1:1:2:1 (ratio 4), and 1:1:1:2 (ratio 5). The sticky trap was placed at a height of 1m from the ground. The sticky trap was a red and yellow insect trap coated with sticky insect glue and natural enemy repellent. A high-density sponge plate was attached to the center of a transparent plastic plate, and then 100 μL of volatiles or blank control (paraffin oil) was pipetted onto the sponge plate and marked with a marker. Sticky traps were randomly arranged in the tea forest, with one sticky trap set up for every 25 m2. Each trap was at least 5 m apart, and different concentrations were separated by at least one row (about 2 m). Each substance was repeated 10 times with different ratios. The number of captured tea horn-breasted leaf beetles was recorded every other day (24 h later). The results are shown in Figure 2. Figure 6 shown.

[0062] Depend on Figure 6 It can be seen that hexyl acetate, nonanal, decanal, and β-ocimene have the best effect on attracting tea horn beetles. Not only female tea horn beetles but also male tea horn beetles are captured on the sticky trap. The ratio 2 (hexyl acetate: nonanal: decanal: β-ocimene = 2:1:1:1) has the largest number of lured tea horn beetles, and the difference with the other four ratios is significant. Therefore, it is recommended to select a composition with a mass ratio of hexyl acetate, nonanal, decanal, and β-ocimene of 2:1:1:1 to attract tea horn beetles.

[0063] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A plant-derived attractant for tea leaf beetles, characterized in that: These include hexyl acetate, nonanal, decanal, and beta-ocimene.

2. The plant-derived attractant for the tea leaf beetle according to claim 1, characterized in that: The mass ratio of hexyl acetate, nonanal, decanal and β-ocimene is 2:1:1:

1.

3. A method for preparing the plant-derived attractant for the tea leaf beetle as claimed in claim 1, characterized in that: The specific steps include: Hexyl acetate, nonanal, decanal and β-ocimene are mixed evenly to obtain the plant-derived attractant for the tea leaf beetle.

4. Use of the plant-derived attractant for tea horn leaf beetles as claimed in claim 1 or the plant-derived attractant for tea horn leaf beetles prepared by the preparation method as claimed in claim 3 in preventing and controlling tea horn leaf beetles.

5. The use according to claim 4, characterized in that: The specific steps include: Add plant-based attractants for tea leaf beetles to conventional traps or use with yellow-red environmentally friendly insect traps.

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