Agricultural liquid fertilizer for tomato planting

Abstract

According to the agricultural liquid fertilizer for tomato planting, bacillus licheniformis, bacillus amyloliquefaciens, bacillus mucilaginosus and bacillus pumilus are subjected to liquid fermentation and then mixed to prepare the novel agricultural liquid fertilizer for tomato planting. According to the present invention, the tomato growth promotion effect is significant, the prevention and control effects on tomato early blight, tomato black spot pathogen, tomato fusarium wilt, tomato gray mold and tomato anthracnose are significant, the tomato survival rate can be improved, the promotion effect is provided, the fruit quality is improved, the important significance is provided for tomato planting, and the economic benefit is significant. The liquid fertilizer can change the soil flora structure, increases the soil fertility and permeability, and plays an important role in soil improvement.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbiology, and particularly relates to an agricultural liquid fertilizer for tomato cultivation. Background Art

[0002] Agricultural liquid fertilizers provide the nutrient elements required for plant growth, and are beneficial to the absorption and transformation of nutrients by crops, playing a role in promoting crop growth, improving crop quality, and increasing crop yields. They are composed of specific nutrients and microorganisms, which can provide the necessary nutrient elements for crops and promote crop growth. At the same time, they can effectively improve the soil microbial environment, reduce the incidence of soil-borne diseases and pests, improve the utilization rate of chemical fertilizers and pesticides, increase crop yields, and improve the quality of agricultural products. As a new type of fertilizer, agricultural liquid fertilizers represent a new development direction in the fertilizer field. The commonly used production strains for existing liquid fertilizers are Bacillus subtilis, actinomycetes, plant growth-promoting rhizobacteria, arbuscular mycorrhizal fungi, methylotrophic bacilli, etc. However, although the commonly used strains can replace raw materials such as chemical fertilizers, reduce the impact of agrochemical products on the environment, increase crop products, promote plant growth, and have high stress resistance, they have defects such as high production costs, low plant epidemic prevention, and average plant growth effects.

[0003] Tomatoes have a unique flavor and are rich in nutrients. They are widely cultivated around the world and are also the main protected vegetables in China. The quality of tomatoes is closely related to human health: tomato fruits contain vitamin C, protein, phenolic substances, and lycopene, which have certain antioxidant effects.

[0004] The wastewater from trehalose production mainly comes from ion-exchanged water, barrel washing water, workshop floor flushing water, condensate water, etc., and is characterized by high concentration, complex composition, and large fluctuations in discharge concentration and water volume. Treating this type of industrial wastewater requires the use of biological methods to degrade organic matter.

[0005] There is currently no precedent in the reported literature for using Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus, along with the wastewater from the trehalose production process and rice flour wastewater, to prepare a new type of agricultural liquid fertilizer. Summary of the Invention

[0006] The purpose of the present invention is to provide a new type of agricultural liquid fertilizer for tomato cultivation. This process separates and treats the wastewater generated during the fermentation process of rice flour wastewater and trehalose production, uses it as a production raw material, can serve as a carbon source and water in the fermentation, and can also solve the problem of environmental pollution caused by rice flour wastewater and industrial wastewater generated by trehalose, reducing pollution and protecting the environment. At the same time, it plays a promoting role in the growth process of tomatoes, achieving the advantages of waste utilization and environmental protection.

[0007] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] A new type of agricultural liquid fertilizer for tomato cultivation, which is obtained by fermenting wastewater from the trehalose production process by microorganisms and rice flour wastewater as fermentation raw materials, and the fermentation product contains a large number of probiotics and antibacterial peptides.

[0009] The microorganisms are Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus and Bacillus pumilus, and the preservation numbers are BNCC221464, BNCC195265, BNCC335819 and BNCC193099 respectively. The four microorganisms are all purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0010] The preparation method of the new type of agricultural liquid fertilizer for tomato cultivation includes the following steps:

[0011] (1) Strain plate and liquid culture

[0012] LB solid medium for Bacillus licheniformis: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0013] Liquid medium for the first-stage seed liquid of Bacillus licheniformis: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0014] Liquid medium for the second-stage seed liquid of Bacillus licheniformis: 10 g of glucose, 5 g of peptone, 3 g of yeast powder, 5 g of sodium chloride, 0.1 g of ferrous sulfate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0015] For the Bacillus licheniformis, first perform plate culture with the solid medium, the culture temperature is 37 °C and the fermentation time is 20 h, then perform shake flask culture with the liquid medium, the culture temperature is 37 °C and the fermentation time is 18 h, then inoculate into the liquid medium at an inoculation amount of 1.5% (V / V) as the first-stage seed liquid, and then inoculate into the liquid medium at an inoculation amount of 2% (V / V) as the second-stage seed liquid.

[0016] LB solid medium for Bacillus amyloliquefaciens: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0017] Liquid medium for the first-stage seed liquid of Bacillus amyloliquefaciens: 5 g of glucose, 3 g of peptone, 3 g of yeast powder, 0.2 g of magnesium chloride, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0018] Bacillus amyloliquefaciens secondary seed liquid medium: 10 g of glucose, 6 g of peptone, 4 g of beef extract, 0.5 g of magnesium chloride, 0.1 g of manganese sulfate, 1 g of dipotassium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0019] For the said Bacillus amyloliquefaciens, first perform plate culture with a solid medium at a culture temperature of 37 °C and a fermentation time of 16 h, then perform shake flask culture with a liquid medium at a culture temperature of 37 °C and a fermentation time of 20 h, then inoculate the liquid medium at an inoculation amount of 2% (V / V) as the primary seed liquid, and then inoculate the liquid medium at an inoculation amount of 3% (V / V) as the secondary seed liquid.

[0020] Bacillus mucilaginosus LB solid medium: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0021] Bacillus mucilaginosus primary seed liquid medium: 5 g of glucose, 5 g of peptone, 5 g of yeast powder, 2 g of potassium dihydrogen phosphate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0022] Bacillus mucilaginosus secondary seed liquid medium: 10 g of glucose, 2 g of ammonium sulfate, 4 g of yeast powder, 3 g of peptone, 0.2 g of zinc sulfate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0023] For the said Bacillus mucilaginosus, first perform plate culture with a solid medium at a culture temperature of 37 °C and a fermentation time of 24 h, then perform shake flask culture with a liquid medium at a culture temperature of 37 °C and a fermentation time of 24 h, then inoculate the liquid medium at an inoculation amount of 3% (V / V) as the primary seed liquid, and then inoculate the liquid medium at an inoculation amount of 3% (V / V) as the secondary seed liquid.

[0024] Bacillus pumilus LB solid medium: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0025] Bacillus pumilus primary seed liquid medium: 5 g of glucose, 5 g of peptone, 5 g of yeast powder, 1 g of potassium dihydrogen phosphate, 1 g of dipotassium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0026] Bacillus pumilus secondary seed liquid medium: 10 g of glucose, 6 g of peptone, 2 g of yeast powder, 0.5 g of calcium chloride, 2 g of disodium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0027] For the Bacillus pumilus, first, it is cultured on a solid medium in a petri dish at a culture temperature of 37°C and a fermentation time of 24 h. Then, it is cultured in a shake flask with a liquid medium at a culture temperature of 37°C and a fermentation time of 22 h. Then, it is inoculated into the liquid medium at an inoculation amount of 2.5% (V / V) as the primary seed liquid, and then inoculated into the liquid medium at an inoculation amount of 5% (V / V) as the secondary seed liquid.

[0028] (2) Take the wastewater generated in the production and processing of rice flour and trehalose, and perform separation and filtration (use a tubular centrifuge to separate the bacteria in the rice flour wastewater and the wastewater generated in the trehalose production process to prevent a large amount of production bacteria from remaining in the waste liquid), and adjust the pH of the wastewater to 7.0 - 8.0.

[0029] (3) Inoculate the seed liquids of four different microorganisms (Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus) into the fermentation medium at an inoculation amount of 2% - 8% respectively. The fermentation temperature is 25°C - 45°C, the aerobic fermentation period is 16 h - 42 h, the aeration ratio is 1 vvm, and the fermentation product contains probiotics and antibacterial peptides.

[0030] The fermentation time is preferably 24 h - 36 h, and more preferably 30 - 36 h.

[0031] The described agricultural liquid fertilizer is characterized in that the treated trehalose waste liquid and rice flour wastewater in step 1) are sterilized at a high temperature of 115°C for 30 min and cooled to below 25°C for standby.

[0032] The trehalose wastewater and the rice flour wastewater are mixed according to a volume ratio of (1 - 5):(1 - 10); preferably, the trehalose wastewater and the rice flour wastewater are mixed according to a volume ratio of 2:1.

[0033] The fermentation medium is 1 - 10 g / L of peptone, 0.1 - 2 g / L of dipotassium hydrogen phosphate, 1 - 5 g / L of sodium chloride, 0.1 - 1 g / L of trace element mixture, 0.1 - 1 g / L of amino acid mixture, and the trehalose wastewater and the rice flour wastewater are supplemented according to a volume ratio of (1 - 5):(1 - 10).

[0034] The described trace element mixture is composed of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate mixed according to a mass ratio of (1 - 10):(1 - 10):(1 - 10):(1 - 5):(1 - 5). Preferably, the mass ratio of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate in the trace element mixture is 1:1:2:1:1.

[0035] The amino acid mixture is composed of aspartic acid, glutamic acid, lysine and tryptophan mixed in a mass ratio of (1-5):(1-10):(1-10):(1-5); preferably, aspartic acid, glutamic acid, lysine and tryptophan are mixed in a mass ratio of 2:1:1:1. The concentration of the amino acid mixture is preferably 0.5 g / L.

[0036] The microorganism is one or a mixture of several of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus and Bacillus pumilus, and the mixture can be mixed in a volume ratio of (1-10):(1-10):(1-10):

[0037] (1-10).

[0038] A novel agricultural liquid fertilizer for tomato planting.

[0039] The fermentation medium contains rice flour wastewater and trehalose production and processing wastewater components, which not only realizes the reuse of waste resources, reduces the environmental pollution of waste materials, greatly reduces the production cost, but also plays a promoting role in the growth process of tomatoes.

[0040] In the present invention, Bacillus licheniformis can be used as a biological control agent to improve soil fertility and promote plant growth; Bacillus amyloliquefaciens can interact with plants through various mechanisms, and can better promote plant growth, control plant diseases and improve crop quality; Bacillus mucilaginosus is a highly active bacterium that can decompose potassium-containing minerals in silicate, aluminosilicate and other ores, and has the functions of dissolving phosphorus, releasing potassium and fixing nitrogen, which can be directly absorbed and utilized by plants, and release soluble calcium, sulfur, magnesium, iron, molybdenum, manganese and other medium and trace elements, which can not only increase soil fertility, but also provide comprehensive nutrient elements that can be absorbed and utilized by crops, and can effectively improve the utilization rate of chemical fertilizers; Bacillus pumilus promotes the growth of plant roots, inhibits the reproduction of pathogens inside and outside plants, reduces pests and diseases, improves and loosens the soil, and improves the utilization rate of chemical fertilizers.

[0041] Using Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus and Bacillus pumilus to produce probiotics and antibacterial peptides by liquid fermentation with trehalose production and processing wastewater and rice flour wastewater respectively, and then mixing them to prepare agricultural liquid fertilizer, and its application in tomato planting has excellent effects. This patent uses the wastewater in the production process of rice flour wastewater and trehalose as fermentation raw materials in the high-density fermentation process. To a certain extent, it can replace carbon sources such as glucose, xylose and starch to reduce production costs. At the same time, its agricultural liquid fertilizer has a promoting effect on tomato planting, meets the demand of resource utilization, and has a market prospect. Therefore, we propose a method for planting tomatoes with a novel agricultural liquid fertilizer.

[0042] The present invention has the following advantages:

[0043] 1. Prepare an agricultural liquid fertilizer by mixing and producing Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus. When used for tomato cultivation, the contents of vitamin C and soluble sugar are significantly increased, and the taste is better than that of tomatoes grown with conventional nitrogen, phosphorus, and potassium fertilizers.

[0044] 2. Compared with the traditional liquid fermentation method, the fermentation of this strain can utilize the rice flour wastewater and the processing wastewater in the production process of trehalose for fermentation. At the same time, the fermentation process is simple. In terms of cost, the fermentation cost is significantly reduced, realizing pollution-free production, which is safe and environmentally friendly. Specific implementation methods

[0045] Through the following examples, the present invention can be better understood. Then, those skilled in the art can easily understand that the specific material ratios, process conditions, and their results described in the examples are only used to illustrate the present invention and should not and will not limit the present invention described in detail in the claims.

[0046] The microorganisms are Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus, with preservation numbers BNCC221464, BNCC195265, BNCC335819, and BNCC193099 respectively, all purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0047] Both the trehalose production and processing wastewater and the rice flour wastewater mainly come from Nanning Bioengineering Co., Ltd. The treatment method is to take the wastewater generated in the production and processing of rice flour and trehalose, and perform separation and filtration (use a tubular centrifuge to separate the bacterial cells from the rice flour wastewater and the wastewater generated in the trehalose production process to prevent a large number of production bacterial cells from remaining in the waste liquid), and adjust the pH of the wastewater to 7.0 - 8.0.

[0048] LB solid medium for Bacillus licheniformis: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0049] Liquid medium for the first-stage seed liquid of Bacillus licheniformis: 10 g of peptone, 5 g of yeast powder, 10 g of sodium chloride, add distilled water to 1000 mL, sterilize at 115 °C for 20 min;

[0050] Liquid medium for the second-stage seed liquid of Bacillus licheniformis: 10 g of glucose, 5 g of peptone, 3 g of yeast powder, 5 g of sodium chloride, 0.1 g of ferrous sulfate, add distilled water to 1000 mL, sterilize at 110 °C for 20 min;

[0051] For the Bacillus licheniformis, first, it is cultured on a solid medium in a petri dish at a culture temperature of 37°C for 20 h. Then, it is cultured in a shake flask with a liquid medium at a culture temperature of 37°C for 18 h. Next, it is inoculated into the liquid medium at an inoculation amount of 1.5% (V / V) as the primary seed liquid, and then inoculated into the liquid medium at an inoculation amount of 2% (V / V) as the secondary seed liquid.

[0052] Solid medium for Bacillus amyloliquefaciens LB: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115°C for 20 min;

[0053] Liquid medium for the primary seed liquid of Bacillus amyloliquefaciens: 5 g of glucose, 3 g of peptone, 3 g of yeast extract, 0.2 g of magnesium chloride, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0054] Liquid medium for the secondary seed liquid of Bacillus amyloliquefaciens: 10 g of glucose, 6 g of peptone, 4 g of beef extract, 0.5 g of magnesium chloride, 0.1 g of manganese sulfate, 1 g of dipotassium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0055] For the Bacillus amyloliquefaciens, first, it is cultured on a solid medium in a petri dish at a culture temperature of 37°C for 16 h. Then, it is cultured in a shake flask with a liquid medium at a culture temperature of 37°C for 20 h. Next, it is inoculated into the liquid medium at an inoculation amount of 2% (V / V) as the primary seed liquid, and then inoculated into the liquid medium at an inoculation amount of 3% (V / V) as the secondary seed liquid.

[0056] Solid medium for Bacillus mucilaginosus LB: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115°C for 20 min;

[0057] Liquid medium for the primary seed liquid of Bacillus mucilaginosus: 5 g of glucose, 5 g of peptone, 5 g of yeast extract, 2 g of potassium dihydrogen phosphate, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0058] Liquid medium for the secondary seed liquid of Bacillus mucilaginosus: 10 g of glucose, 2 g of ammonium sulfate, 4 g of yeast extract, 3 g of peptone, 0.2 g of zinc sulfate, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0059] The Bacillus mucilaginosus is first cultured on a solid medium in a petri dish at a culture temperature of 37°C for a fermentation time of 24 h. Then, it is cultured in a shake flask with a liquid medium at a culture temperature of 37°C for a fermentation time of 24 h. Subsequently, it is inoculated into the liquid medium at an inoculation amount of 3% (V / V) as the primary seed liquid, and then inoculated into the liquid medium at an inoculation amount of 3% (V / V) as the secondary seed liquid.

[0060] The solid medium for Bacillus pumilus LB: 10 g of peptone, 5 g of yeast extract, 10 g of sodium chloride, 20 g of agar, add distilled water to 1000 mL, sterilize at 115°C for 20 min;

[0061] The liquid medium for the primary seed liquid of Bacillus pumilus: 5 g of glucose, 5 g of peptone, 5 g of yeast extract, 1 g of potassium dihydrogen phosphate, 1 g of dipotassium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0062] The liquid medium for the secondary seed liquid of Bacillus pumilus: 10 g of glucose, 6 g of peptone, 2 g of yeast extract, 0.5 g of calcium chloride, 2 g of disodium hydrogen phosphate, add distilled water to 1000 mL, sterilize at 110°C for 20 min;

[0063] The Bacillus pumilus is first cultured on a solid medium in a petri dish at a culture temperature of 37°C for a fermentation time of 24 h. Then, it is cultured in a shake flask with a liquid medium at a culture temperature of 37°C for a fermentation time of 22 h. Subsequently, it is inoculated into the liquid medium at an inoculation amount of 2.5% (V / V) as the primary seed liquid, and then inoculated into the liquid medium at an inoculation amount of 5% (V / V) as the secondary seed liquid.

[0064] Example 1

[0065] In this example, the fermentation of four strains (Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus) with different trace element ratios was investigated. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a shaking flask with a liquid medium. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loop of bacteria respectively), they were inoculated into the liquid medium as the primary seed liquid. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed liquid. Then, their respective seed liquid inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2%, 2%, 2.5%, and 2.5% respectively) were inoculated into the fermentation medium respectively for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, trace element mixture 0.1 - 1 g / L, amino acid mixture 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to the volume ratio (1:1). Mix and stir, sterilize at 115 °C for 20 minutes. Inoculate into the prepared fermentation medium, at a temperature of 37 °C, carry out aerobic fermentation for 24 h. The mass ratios of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate in the trace element mixture were 1:1:1:1:1, 2:1:1:1:1, 1:2:1:1:1, 1:1:2:1:1, 1:1:1:2:1, and 1:1:1:1:2 to study their effects on fermentation; after determining the optimal ratio of trace element metal ions, the effects of different concentrations of the trace element mixture (0.1 g / L, 0.3 g / L, 0.5 g / L, 0.7 g / L, 0.9 g / L, 1 g / L) on fermentation were investigated, and the colony count and antibacterial peptides in the liquid fermentation broth were measured. The results are shown in Table 1 and Table 2.

[0066] Table 1 Effects of different trace element ratios on the fermentation broth of strains

[0067]

[0068]

[0069] Table 2 Effects of different concentrations of the trace element mixture on the colony count in the fermentation broth of two strains

[0070]

[0071] Example 2

[0072] In this example, the effects of different amino acid element ratios on the single fermentation of two strains were investigated. Bacillus subtilis and Bacillus megaterium were cultured on a solid medium in petri dishes, and then cultured in a liquid medium in shake flasks. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loopful of bacteria respectively), they were inoculated into the liquid medium as the primary seed liquid. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed liquid. Then, their respective seed liquid inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2%, 2%, 2.5%, and 2.5% respectively) were inoculated into the fermentation medium respectively for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixed solution of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, amino acid mixed solution 0.1 - 1 g / L, and the trehalose wastewater and rice flour wastewater were made up according to the volume ratio (1:1). Mix and stir, sterilize at 115 °C for 20 minutes. Inoculate into the prepared fermentation medium, at a temperature of 37 °C, carry out aerobic fermentation for 24 h. Mix and stir, sterilize at 115 °C for 20 minutes. Inoculate into the prepared fermentation medium, at a temperature of 37 °C, carry out aerobic fermentation for 24 h. The mass ratios of aspartic acid, glutamic acid, lysine, and tryptophan in the amino acid mixed solution were 1:1:1:1, 2:1:1:1, 1:2:1:1, 1:1:2:1, 1:1:1:2 respectively to study their effects on fermentation; after determining the optimal ratio of different amino acid contents, the effects of concentrations of 0.1 g / L, 0.3 g / L, 0.5 g / L, 0.7 g / L, 0.9 g / L, and 1 g / L on fermentation were investigated, and the colony counts and antibacterial peptides in the liquid fermentation broth were measured. The results are shown in Table 3 and Table 4.

[0073] Table 3 Effects of different amino acid mass ratios on the fermentation of two strains

[0074]

[0075] Table 4 Effects of different amino acid mixed solution concentrations on the fermentation of two strains

[0076]

[0077]

[0078] Example 3

[0079] In this example, the influence of fermentation time on the fermentation situation was investigated. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a liquid medium in shake flasks. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loopful of bacteria respectively), they were inoculated into the liquid medium as the primary seed liquid. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed liquid. Then, their respective seed liquids (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2%, 2%, 2.5%, and 2.5% respectively) were inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixed solution of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixed solution of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan with a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to the volume ratio (1:1). Mix and stir, sterilize at 115 °C for 20 minutes. Inoculate into the prepared fermentation medium, at a temperature of 37 °C, carry out aerobic fermentation for 18 - 42 h. Measure the colony count and antibacterial peptides in the liquid fermentation broth, and the results are shown in Table 5.

[0080] Table 5 Influence of Different Fermentation Times on the Fermentation of Strains

[0081]

[0082]

[0083] Example 4

[0084] This example studied the effects of different inoculation amounts (1% - 10%) on the colony count and antibacterial peptides of the mixed bacterial liquid; Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on solid medium in petri dishes, then cultured in shake flasks with liquid medium. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were each one loopful of bacteria), they were inoculated into the liquid medium as the primary seed liquid. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed liquid. Then, their respective seed liquids (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2.5%, 3%, 3.5%, and 3% respectively) were inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixed solution of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixed solution of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan with a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to the volume ratio (1:1). The colony count and antibacterial peptide content of the mixed bacterial liquid were measured, and the results are shown in Table 6.

[0085] Table 6 Effects of Different Inoculation Amounts on Colony Count and Antibacterial Peptides

[0086]

[0087]

[0088] Example 5

[0089] In this example, the mixing volume ratios of different strains were investigated. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a shaking flask with a liquid medium. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loopful of bacteria respectively), they were inoculated into the liquid medium as the primary seed liquid. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed liquid. Then, their respective seed liquids (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2.5%, 3%, 3.5%, and 3% respectively) were inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixed solution of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixed solution of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan with a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to the volume ratio (1:1). The mixing volume ratios of the Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus bacterial liquid were (1:1:1:1), (2:1:1:1), (1:2:1:1), (1:1:2:1), (1:1:1:2) respectively. The colony counts and antibacterial peptide contents of the mixed bacterial liquid were measured, and the results are shown in Table 7.

[0090] Table 7 Effects of different mixing volume ratios of bacterial liquid on colony counts and antibacterial peptides

[0091] Mixing volume ratio Total viable count, cfu / mL Antibacterial peptide titer, U / mL 1:1:1:1 <![CDATA[2.65×10 9 > 865.8 2:1:1:1 <![CDATA[7.8×10 9 > 1045.5 1:2:1:1 <![CDATA[9.5×10 9 > 1302.3 1:1:2:1 <![CDATA[6.9×10 9 > 996.8 1:1:1:2 <![CDATA[4.7×10 9 > 879.4

[0092] Experimental Example 6

[0093] This example examines the effect of the ratio of trehalose processing wastewater to rice flour wastewater on the fermentation products. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a liquid medium in shake flasks. Then, they were inoculated into the liquid medium as the primary seed solution according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loopful of bacteria each), and then inoculated into the liquid medium as the secondary seed solution according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively). Then, their respective seed solutions (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2.5%, 3%, 3.5%, and 3% respectively) were inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixed solution of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixed solution of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan with a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to a volume ratio of (1 - 5):(1 - 10). The volume ratio of the mixed solutions of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus was 2:1:1:1, and the experimental results are shown in Table 8.

[0094] Table 8 Effect of the ratio of trehalose processing wastewater to rice flour wastewater on the fermentation products

[0095]

[0096] Example 7

[0097] This example examines the effect of different fermentation media on the fermentation products. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a liquid medium in shake flasks. Then, they were inoculated into the liquid medium as the primary seed solution according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were one loopful of bacteria each), and then inoculated into the liquid medium as the secondary seed solution according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively). Then, their respective seed solutions (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2.5%, 3%, 3.5%, and 3% respectively) were inoculated into the fermentation medium for fermentation experiments, and the colony count and antibacterial peptide content of the mixed bacterial solution were measured. The results are shown in Table 9.

[0098] Fermentation medium ①: Peptone 6 g / L, Dipotassium hydrogen phosphate 2 g / L, Sodium chloride 3 g / L, Trace element (a mixed solution of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, Amino acid (a mixed solution of aspartic acid, glutamic acid, lysine, and tryptophan with mass ratios of 2:1:1:1 respectively) 0.5 g / L, Trehalose wastewater and rice flour wastewater are supplemented to make up the volume according to a ratio of 2:1. The volume ratio of the mixed liquid of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus bacterial solutions is 2:1:1:1. Mix and stir, and sterilize at 115 °C for 20 minutes.

[0099] Fermentation medium ②: Glucose 10 g / L, Peptone 6 g / L, Dipotassium hydrogen phosphate 2 g / L, Sodium chloride 3 g / L, Trace element (a mixed solution of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, Amino acid (a mixed solution of aspartic acid, glutamic acid, lysine, and tryptophan with mass ratios of 2:1:1:1 respectively) 0.5 g / L, Made up with tap water. The volume ratio of the mixed liquid of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus bacterial solutions is 2:1:1:1. Mix and stir, and sterilize at 115 °C for 20 minutes.

[0100] Fermentation medium ③: Peptone 6 g / L, Dipotassium hydrogen phosphate 2 g / L, Sodium chloride 3 g / L, Trace element (a mixed solution of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, Amino acid (a mixed solution of aspartic acid, glutamic acid, lysine, and tryptophan with mass ratios of 2:1:1:1 respectively) 0.5 g / L, Made up with rice flour wastewater. The volume ratio of the mixed liquid of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus bacterial solutions is 2:1:1:1. Mix and stir, and sterilize at 115 °C for 20 minutes.

[0101] Fermentation medium ④: Peptone 6 g / L, Dipotassium hydrogen phosphate 2 g / L, Sodium chloride 3 g / L, Trace element (a mixed solution of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, Amino acid (a mixed solution of aspartic acid, glutamic acid, lysine, and tryptophan with mass ratios of 2:1:1:1 respectively) 0.5 g / L, Made up with trehalose wastewater. The volume ratio of the mixed liquid of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus bacterial solutions is 2:1:1:1. Mix and stir, and sterilize at 115 °C for 20 minutes.

[0102] Table 9 Effects of different fermentation media on fermentation

[0103]

[0104]

[0105] Example 8

[0106] This example examines the effects of single-strain fermentation broth and mixed-strain fermentation broth on tomato cultivation. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were cultured on a solid medium in petri dishes, and then cultured in a liquid medium in shake flasks. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were each one loopful of bacteria), they were inoculated into the liquid medium as the primary seed solution. Then, according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 1%, 1%, 1.5%, and 1.5% respectively), they were inoculated into the liquid medium as the secondary seed solution. Then, their respective seed solutions (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus were 2.5%, 3%, 3.5%, and 3% respectively) were inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixture of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate with a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixture of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan with a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater were supplemented according to the volume ratio (1:1). The mixed volume ratios of the bacterial solutions of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus in the mixed-strain fermentation broth were (2:1:1:1) respectively. Among them, the spraying amount was 2% (the dilution factor was 1 L of tap water added to 10 mL of agricultural liquid fertilizer for drip irrigation of vegetables), and the experimental results are shown in Table 10.

[0107] Table 10 Effects of Single-Strain Fermentation Broth and Mixed-Strain Fermentation Broth on Tomato Cultivation

[0108] Fermentation broth treatment method Tomato vitamin C (mg / kg) Soluble sugar (%) Bacillus licheniformis fermentation broth 711.3 24.34 Bacillus amyloliquefaciens fermentation broth 859.9 29.57 Bacillus mucilaginosus fermentation broth 426.8 11.26 Bacillus pumilus fermentation broth 560.5 20.13 Mixed bacteria fermentation broth 1132.7 39.15

[0109] Example 9

[0110] This example examines the cultivation of tomatoes. Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus are cultured on a solid medium in petri dishes, and then cultured in a shaking flask with a liquid medium. Then, they are inoculated into the liquid medium according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus are each one loopful of bacteria) as the primary seed liquid. Then, they are inoculated into the liquid medium according to their respective inoculation amounts (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus are 1%, 1%, 1.5%, and 1.5% respectively) as the secondary seed liquid. Then, their respective seed liquids (the inoculation amounts of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus are 2.5%, 3%, 3.5%, and 3% respectively) are inoculated into the fermentation medium for fermentation experiments. Fermentation medium: peptone 6 g / L, dipotassium hydrogen phosphate 2 g / L, sodium chloride 3 g / L, a mixture of trace elements (magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, zinc sulfate in a mass ratio of 2:1:1:1:1) 0.5 g / L, a mixture of amino acids (aspartic acid, glutamic acid, lysine, and tryptophan in a mass ratio of 2:1:1:1) 0.5 g / L, and the trehalose wastewater and rice flour wastewater are supplemented according to a volume ratio of (1:1). The mixed volume ratios of the bacterial liquids of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus are (2:1:1:1) respectively, and an antibacterial test is carried out on tomato pathogens. The antibacterial effects are significantly different, and the experimental results are shown in Table 11.

[0111] Table 11 Antibacterial effects of the new type of agricultural liquid fertilizer on tomato pathogens

[0112] Tomato pathogenic bacteria Inhibition zone (mm) Early blight of tomato 32.6±0.2 Alternaria alternata f. sp. lycopersici 27.3±0.3 Fusarium wilt of tomato 29.9±0.4 Botrytis cinerea of tomato 35.6±0.2 Anthracnose of tomato 26.8±0.1

[0113] Example 10

[0114] This invention examines the application of the agricultural liquid fertilizer on tomatoes, and studies the growth and development and yield of tomatoes with the above-mentioned agricultural liquid fertilizer. Three treatments are set in the experiment, repeated three times, with a total of nine plots, randomly arranged. The plot area is 4 m × 8 m = 32 m 2 . Conventional fertilization + spraying 500 mL of microbial inoculant (100-fold dilution with water) / 666.7 m 2 . Conventional fertilization + spraying 500 mL of inactivated matrix (100-fold dilution with water) / 666.7 m 2· times; conventional fertilization. Treatment 3 is conventional fertilization. The tomatoes are planted in a large arched shed. According to the field observation after spraying the agricultural liquid fertilizer, compared with Treatment 2 (inactivated substrate) and Treatment 3 (conventional fertilization), the growth of tomatoes in Treatment 1 (test liquid fertilizer) is stronger, the leaf color is emerald green, the leaves are thick, the plants are robust, and the disease resistance is enhanced. In Treatment 1, the transverse diameter of the fruit increases by 0.5 cm compared with Treatment 2, the longitudinal diameter increases by 0.4 cm, the single fruit weight increases by 7.8 g, the commercial rate increases by 6.5%, and the umbilical rot disease decreases by 3.0%; compared with Treatment 3, the transverse diameter of the fruit in Treatment 1 increases by 0.7 cm, the longitudinal diameter increases by 0.6 cm, the single fruit weight increases by 8.4 g, the commercial rate increases by 8.2%, and the umbilical rot disease decreases by 3.2%. The average yield increase of Treatment 1 (test fertilizer) and Treatment 2 (inactivated substrate) is 358.3 Kg / 666.7m 2 , and the yield increase rate is 7.6%; compared with Treatment 3 (conventional fertilization), the average increase is 435.4 Kg / 666.7m 2 Kg, and the yield increase rate is 9.4%. As shown in Tables 12 and 13.

[0115] Table 12 Effects of different treatments on the biological traits and disease resistance of tomatoes

[0116]

[0117] Table 13 Tomato yield table

[0118]

[0119] The technical solutions provided by the embodiments of the present invention have been introduced in detail above. Specific examples are used in this article to elaborate on the principles and implementation manners of the embodiments of the present invention. The descriptions of the above embodiments are only applicable to help understand the principles of the embodiments of the present invention. At the same time, for those of ordinary skill in the art, according to the embodiments of the present invention, there will be changes in the specific implementation manners and application scopes. In summary, the content of this specification should not be construed as a limitation to the present invention.

Claims

1. An agricultural liquid fertilizer for tomato cultivation, characterized in that, The agricultural liquid fertilizer is obtained by fermenting a variety of Bacillus strains using rice flour wastewater and trehalose wastewater as fermentation raw materials, and the fermentation product contains a large number of probiotics and antibacterial peptides.

2. The agricultural liquid fertilizer for tomato cultivation according to claim 1, wherein The Bacillus strains are one or a mixture of more than one of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus.

3. The agricultural liquid fertilizer for tomato cultivation according to claim 1, characterized in that, The number of microbial colonies in the liquid fertilizer ≥ 7.8×10 9 cfu / mL, and the titer of antibacterial peptide ≥ 865.8 U / mL.

4. The agricultural liquid fertilizer for tomato planting according to claim 1, characterized in that, The trehalose wastewater and rice flour wastewater are mixed as fermentation raw materials in a volume ratio of (1-5):(1-10).

5. The agricultural liquid fertilizer for tomato cultivation according to claim 1, wherein, The fermentation medium is 1-10 g / L of peptone, 0.1-2 g / L of dipotassium hydrogen phosphate, 1-5 g / L of sodium chloride, 0.1-1 g / L of trace element mixture, 0.1-1 g / L of amino acid mixture, and the mixture of trehalose wastewater and rice flour wastewater is added to make up the volume.

6. The agricultural liquid fertilizer for tomato cultivation according to claim 2, wherein The Bacillus strains are a mixture of Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus mucilaginosus, and Bacillus pumilus in a mass ratio of (1-10):(1-10):(1-10):(1-10).

7. The agricultural liquid fertilizer for tomato cultivation according to claim 5, characterized in that, The trace element mixture is a mixture of magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate, and zinc sulfate in a mass ratio of (1-10):(1-10):(1-10):(1-5):(1-5).

8. The agricultural liquid fertilizer for tomato cultivation according to claim 5, characterized in that, The amino acid mixture is a mixture of aspartic acid, glutamic acid, lysine, and tryptophan in a mass ratio of (1-5):(1-10):(1-10):(1-5).

9. The preparation method of the agricultural liquid fertilizer for tomato planting according to any one of claims 1-8, characterized in that, It includes the following steps: 1) Take the wastewater generated in the production and processing of rice flour and trehalose, carry out separation and filtration, and adjust the pH of the wastewater to 7.0-8.0; 2) Inoculate the Bacillus seed liquid into the fermentation medium at an inoculation amount of 2%-8%, the fermentation temperature is 25 °C - 45 °C, the aerobic fermentation period is 16 h - 32 h, the aeration ratio is 1 vvm, and the fermentation product contains probiotics and antibacterial peptides.

10. Application of the agricultural liquid fertilizer for tomato planting according to any one of claims 1-8 in tomato planting.

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