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Biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid

A technology of pentachlorophenol and anaerobic microorganisms, applied in the field of biochar preparations, can solve the problems of long time consumption, limitation, complicated extraction methods, etc., and achieve the effects of environment-friendly products and easy-to-obtain sources.

Active Publication Date: 2016-03-23
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] However, the extraction method of humin is very cumbersome and time-consuming, and each extraction and purification takes one to two months
Also, the electronic mediation effects of humin are often limited by soil or sediment sources

Method used

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  • Biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid
  • Biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A biochar preparation capable of mediating the anaerobic reduction dechlorination of pentachlorophenol, which is prepared by mixing the following components: 1 L of inorganic salt medium, 0.5 mg of anaerobic microorganisms, 0.6 g of sodium formate, 1 mL of vitamin solution, and Caragana biochar 4g.

[0030] In terms of mass percentage, the anaerobic microorganisms are made by mixing the following strains: 38% of Sulfuromonas, 32% of Bacteroides, 20% of Clostridium bienzyme, 4% of Dehalogenobacillus, and 6% of Desulfovibrio .

[0031] The composition of the inorganic salt medium is: NH 4 Cl1.0g / L, CaCl 2 2H 2 O0.05g / L, MgCl 2 ·6H 2 O0.1g / L, K 2 HPO 4 0.4g / L, SL-10 trace element solution 1mL / L, Se / W solution 1mL / L, pH7.2 MOPs buffer 15mmol / L, water balance.

[0032] The composition of each liter of SL-10 trace element solution is: HCl (7.7M) 10.0mL, FeCl 2 4H 2 O1.5g, ZnCl 2 70.0mg, MnCl 2 4H 2 O100.0mg, H 3 BO 3 6.0 mg, CoCl 2 ·6H 2 O196.0mg, CuCl 2 2H ...

Embodiment 2

[0036] A biochar preparation capable of mediating the anaerobic reduction dechlorination of pentachlorophenol, which is prepared by mixing the following components: 1 L of inorganic salt medium, 1 mg of anaerobic microorganisms, 0.8 g of sodium formate, 2 mL of vitamin solution, and 6 g of caragana biochar .

[0037] In terms of mass percentage, the anaerobic microorganisms are made by mixing the following strains: 40% of Sulfuromonas, 31% of Bacteroides, 19% of Clostridium bienzyme, 6% of Dehalogenobacillus, and 4% of Desulfovibrio .

[0038] Others are with embodiment 1.

Embodiment 3

[0040] A biochar preparation capable of mediating the anaerobic reduction dechlorination of pentachlorophenol, which is prepared by mixing the following components: 1 L of inorganic salt medium, 0.8 mg of anaerobic microorganisms, 0.69 g of sodium formate, 1 mL of vitamin solution, and Caragana biochar 5g.

[0041] In terms of mass percentage, the anaerobic microorganisms are made by mixing the following strains: 39.2% of Sulfuromonas, 31.7% of Bacteroides, 19.5% of Clostridium bienzyme, 4.8% of Dehalogenobacillus, and 4.8% of Desulfovibrio .

[0042] Others are with embodiment 1.

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Abstract

The invention discloses a biochar preparation capable of mediating in anaerobic reductive dechlorination of chlorophenasic acid. The biochar preparation is formed by mixing 1 L of inorganic salt culture media, 0.5-1 mg of anaerobes, 0.6-0.8 g of sodium formate, 1-2 mL of a vitamin solution and 4-6 g of caragana korshinskii biochar. Sources of the raw materials of the biochar preparation are easy to obtain, and the biochar preparation is environmentally friendly, non-toxic and capable of effectively mediating in anaerobic reductive dechlorination of the chlorophenasic acid, and therefore a new way is provided for degrading the chlorophenasic acid.

Description

technical field [0001] The invention relates to the field of biodegradation of organic matter, in particular to a biochar preparation capable of mediating anaerobic reduction dechlorination of pentachlorophenol. Background technique [0002] Halogenated aromatic compounds and their derivatives are chemical raw materials, organic synthesis intermediates and organic solvents widely used in chemical industry, medicine, leather, electronics and other industries. Because of its extensive pollution, high toxicity, difficult to degrade, and most of them will cause teratogenic, carcinogenic, and mutagenic effects on the human body, it has attracted widespread attention. Among them, Pentachlorophenol (PCP) is a typical halogenated aromatic compound, which has been widely used as an insecticide, wood preservative and fungicide, causing worldwide soil and groundwater pollution. Because PCP poses serious threats to human health (high toxicity to the liver and kidneys, and the risk of c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02A62D101/28
CPCA62D3/02A62D2101/28
Inventor 杨生茂章春芳钟哲科刘玉学李智灵张冬冬吕豪豪汪玉瑛张宁
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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