Bacterial strain for degradation of 2,4,6-trichlorophenol and screening separation method thereof

A technology of trichlorophenol and separation method, applied in the field of degradation 2, can solve the problems of inability to meet the large-scale treatment requirements of chlorophenols, low purity of bacillus, low yield, etc., and achieves a simple method, high yield and high purity. Effect

Pending Publication Date: 2016-11-16
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] To sum up, Bacillus spindle-shaped Lysinica can degrade chlorophenols organic matter through reductive dechlorination under anaerobic conditions, and the degradation effect is good. Low, low output, unable to meet the large-scale processing needs of chlorophenols

Method used

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  • Bacterial strain for degradation of 2,4,6-trichlorophenol and screening separation method thereof
  • Bacterial strain for degradation of 2,4,6-trichlorophenol and screening separation method thereof
  • Bacterial strain for degradation of 2,4,6-trichlorophenol and screening separation method thereof

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Effect test

Embodiment 1

[0058] Collect the activated sludge in the internal circulation anaerobic reactor used to treat papermaking wastewater in a paper mill, measure 1L of semi-fluid activated sludge and add it to the domestication culture bottle, then add 1L of the domestication medium to adjust the pH to 7.0 , the acclimatization system after pH adjustment was enriched and acclimatized at 30°C in a strict anaerobic environment until the concentration of 2,4,6-trichlorophenol in the acclimatized system reached 800mg / L, and acclimatized sludge.

[0059] The domesticated sludge was inoculated in the solid screening medium supplemented with 8mg / L 2,4,6-trichlorophenol according to the dilution gradient, and three kinds of 2,4,6-trichlorophenol degradable Purified strains: A1, A2, A3.

[0060] The purified strains A1, A2, and A3 were respectively inoculated in the degradation medium supplemented with 90 mg / L 2,4,6-trichlorophenol. After testing, the degradation of the purified strain A1 to 2,4,6-tric...

Embodiment 2

[0062] Collect the activated sludge in the internal circulation anaerobic reactor used to treat papermaking wastewater in a paper mill, measure 1L of semi-fluid activated sludge and add it to the domestication culture bottle, then add 1L of domestication medium to adjust the pH to 7.2 , the domestication system after pH adjustment was enriched and domesticated at 40°C in a strict anaerobic environment until the concentration of 2,4,6-trichlorophenol in the domesticated system reached 800mg / L, and the domesticated sludge.

[0063]The domesticated sludge was inoculated in the solid screening medium supplemented with 12mg / L 2,4,6-trichlorophenol according to the dilution gradient, and five kinds of 2,4,6-trichlorophenol degradable Purified strains: C1, C2, C3, C4, C5.

[0064] The purified strains C1, C2, C3, C4, and C5 were respectively inoculated in the degradation medium supplemented with 110 mg / L 2,4,6-trichlorophenol. After testing, the purified strain C2 was resistant to 2...

Embodiment 3

[0066] The activated sludge in the anaerobic treatment reactor in the internal circulation of wastewater from a paper mill was collected, and 1L of semi-fluid activated sludge was added to the domestication culture bottle, and then 1L of the domestication medium was added to adjust the pH to 7.0. Fill the acclimatization culture bottle with nitrogen, and then seal the mouth of the acclimatization culture bottle with a rubber stopper with an air guide tube, so that the end of the air guide tube inside the bottle is inserted into the acclimatization system, and the end of the air guide tube outside the bottle is inserted into the sodium hydroxide solution Perform a liquid seal. The whole set of acclimatization device is placed in an anaerobic biochemical incubator for cultivation at 30-40°C. Transfer the culture to a solid medium supplemented with 50 mg / L of 2,4,6-trichlorophenol according to the inoculum size of 10%, and put it back into the anaerobic biochemical incubator to c...

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Abstract

The invention discloses a screening separation method of a bacterial strain for degradation of 2,4,6-trichlorophenol, and relates to the technical field of treatment of organic pollutants. The method comprises the steps: collecting activated sludge in a papermaking wastewater inner circulation anaerobic reactor, carrying out enrichment acclimation in a strictly anaerobic environment, then inoculating solid screening culture mediums having addition of 2,4,6-trichlorophenol respectively with the acclimated sludge according to the dilution gradient, and separating to obtain various purified bacterial strains having the ability to degrade 2,4,6-trichlorophenol; and finally, respectively inoculating degradation culture mediums having addition of 2,4,6-trichlorophenol with the various purified bacterial strains, and screening to obtain the bacterial strain having the highest degradation efficiency on 2,4,6-trichlorophenol. The method is simple, and the obtained spindle-shaped lysine bacillus has relatively high purity and high yield. The invention also provides the bacterial strain for degradation of 2,4,6-trichlorophenol; the bacterial strain can degrade chlorophenol organic matters through reductive dechlorination under anaerobic conditions, and has good degradation effect.

Description

technical field [0001] The invention relates to the technical field of organic pollutant treatment, and in particular to a bacterial strain for degrading 2,4,6-trichlorophenol and a screening and isolation method thereof. Background technique [0002] Chlorophenols are chlorinated hydrocarbons, insecticides or anti-microbial reagents used for non-agricultural insecticide. The concentration threshold of chlorophenols that are toxic to humans is 40ppm, and the threshold of toxic effects on fish is only Therefore, chlorophenols are very likely to cause water pollution, and are also listed as priority pollutants by the US EPA and key pollutants in China. [0003] At present, the treatment methods for p-chlorophenols mainly include ultrafiltration, chemical precipitation, and biological methods. Among them, the ultrafiltration and chemical precipitation methods have the disadvantages of high energy consumption, high cost, and secondary pollution caused by chemical addition. Trea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/36C12N1/02C02F3/34C02F3/28C12R1/07C02F101/36
CPCC12N1/20C02F3/28C02F3/34C02F2101/36C12N1/02C12N1/36
Inventor 陈天明殷春涛丁成杨文骏肖波金建祥许安盈张珍珍
Owner YANCHENG INST OF TECH
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