Biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls

A technology of polychlorinated biphenyls and biological agents, applied in the direction of microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of long time-consuming, cumbersome extraction methods, restrictions, etc., and achieve the effect of environmental friendliness of products

Active Publication Date: 2018-04-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the extraction method of humin is very cumbersome and time-consuming, and each extraction and purification takes one to two months
Also, the electronic mediation effects of humin are often limited by soil or sediment sources

Method used

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  • Biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls
  • Biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls
  • Biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A biological preparation capable of mediating anaerobic reductive dechlorination of polychlorinated biphenyls, made by mixing the following components: 1L of inorganic salt medium, 1mg of anaerobic microorganisms, 1.5ml of sodium lactate, 1mL of vitamin solution, and 4g of rapeseed biochar .

[0033] In terms of mass percentage, the anaerobic microorganisms are composed of the following species: Desulfuromonas sp. 43.5%, Desulfitobacterium sp. 23.6%, Exiguobacterium sp. 3.8%, Citrobacter sp. .) 6.2%, Alkaliphilus sp. 5.1%, Pseudomonas sp. 1.8%, Dehalobacter sp. 6.5%, Bellilinea sp. 3.6%, Dehalogenimonas sp. 5.9%.

[0034] The formula of the inorganic salt medium is: NH 4 Cl 1.0g / L, CaCl 2 2H 2 O 0.05g / L, MgCl 2 6H2O0.1g / L, K 2 HPO 4 0.4g / L, Se / W solution 1mL / L, SL-10 trace element solution 1mL / L, pH7.2 MOPS buffer 10mmol / L, vitamin solution 0.02ml / L, water balance.

[0035] The composition of each liter of Se / W solution is: NaOH 0.5g, NaSeO 3 ·5H 2 O 3 mg, Na ...

Embodiment 2

[0039] A biological preparation capable of mediating anaerobic reductive dechlorination of polychlorinated biphenyls, made by mixing the following components: 1L of inorganic salt medium, 2mg of anaerobic microorganisms, 2.5ml of sodium lactate, 2mL of vitamin solution, and 6g of rapeseed biochar .

[0040] In terms of mass percent, the anaerobic microorganisms are composed of the following bacterial species: Desulfuromonas sp. 45.2%, Desulfitobacterium sp. 25.8% Exiguobacterium sp. 5.3%, Citrobacter sp. ) 6.5%, Alkaliphilus sp. 4.9%, Pseudomonas sp. 2.3%, Dehalobacter sp. 3.8%, Bellilinea sp. 3.4%, Dehalogenimonas sp. 2.8%.

[0041] Others are with embodiment 1.

Embodiment 3

[0043] A biological preparation capable of mediating anaerobic reductive dechlorination of polychlorinated biphenyls, made by mixing the following components: 1L of inorganic salt medium, 1.5mg of anaerobic microorganisms, 2ml of sodium lactate, 1.5mL of vitamin solution, and biochar of rape stalks 5g.

[0044] In terms of mass percentage, the anaerobic microorganisms are composed of the following bacterial species: Desulfuromonas sp. 45.5%, Desulfitobacterium sp. 21.6% Exiguobacterium sp. 5.2%, Citrobacter sp. ) 8.1%, Alkaliphilus sp. 4.7%, Pseudomonas sp. 1.8%, Dehalobacter sp. 3.5%, Bellilinea sp. 4.1%, Dehalogenimonas sp. 5.5%.

[0045] Others are with embodiment 1.

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Abstract

The invention discloses a biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls. The biochar preparation is prepared from the following components of, by weight, 1 L of an inorganic salt culture medium, 1.5-2.5 Ml of sodium lactate, 1-2 mL of a vitamin solution, 1-2 mg of an anaerobic microorganism, and 4-6 g of rape stem biochar. The biological preparation has the characteristics of being environment-friendly, non-toxic and the like, anaerobic reductive dechlorination of the polychlorinated biphenyls can be effectively mediated, and the biological preparation provides a new way for degrading of the polychlorinated biphenyls.

Description

technical field [0001] The invention specifically relates to a biochar preparation capable of mediating anaerobic reduction dechlorination of polychlorinated biphenyls, and relates to the field of organic matter biodegradation. Background technique [0002] Polychlorinated biphenyls (PCBs) is a general term for a class of chlorinated aromatic compounds that are replaced by chlorine atoms to varying degrees of hydrogen on biphenyls, including 209 homologues. Due to its good chemical stability, flame retardancy and insulation, it has been widely used in electrical equipment (such as transformers, varactors, etc.) as insulating media, carbon paper, ink, paint, lubricants, plasticizers, etc. Toxicity, environmental persistence, bioaccumulation, and long-distance migration. Because the structure of PCBs is very stable, it is difficult to be degraded in the environment. At the same time, it has low water solubility and high fat solubility, and can be enriched from the surrounding ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A62D3/02C12N1/20C12R1/01C12R1/38
CPCA62D3/02A62D2101/22C12N1/20
Inventor 张宁章春芳张冬冬张志超余新威
Owner ZHEJIANG UNIV
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