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A biochar formulation for mediating anaerobic reductive dechlorination of polychlorinated biphenyls

A technology for polychlorinated biphenyls and biological preparations, applied in the directions of microorganisms, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of long time, cumbersome extraction methods, limitations, etc., and achieve the effect of environmentally friendly products.

Active Publication Date: 2020-06-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the extraction method of humin is very cumbersome and time-consuming, and each extraction and purification takes one to two months
Also, the electronic mediation effects of humin are often limited by soil or sediment sources

Method used

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  • A biochar formulation for mediating anaerobic reductive dechlorination of polychlorinated biphenyls
  • A biochar formulation for mediating anaerobic reductive dechlorination of polychlorinated biphenyls
  • A biochar formulation for mediating anaerobic reductive dechlorination of polychlorinated biphenyls

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A biological preparation that can mediate the anaerobic reduction and dechlorination of PCBs, which is made by mixing the following components: 1L inorganic salt medium, 1mg anaerobic microorganisms, 1.5ml sodium lactate, 1mL vitamin solution, 4g rape stalk biochar .

[0033] In terms of mass percentage, the anaerobic microorganisms consist of the following strains: Desulfuromonassp. 43.5%, Desulfitobacterium sp. 23.6%, Exiguobacterium sp. 3.8%, Citrobacter sp. .) 6.2%, Alkaliphilus sp. 5.1%, Pseudomonassp. 1.8%, Dehalobacters p. 6.5%, Bellilinea sp. 3.6%, Dehalogenimonas sp. 5.9%.

[0034] The formula of the inorganic salt culture medium is: NH 4 Cl 1.0g / L, CaCl 2 ·2H 2 O 0.05g / L, MgCl 2 ·6H2O0.1g / L, K 2 HPO 4 0.4g / L, Se / W solution 1mL / L, SL-10 trace element solution 1mL / L, pH7.2 MOPS buffer solution 10mmol / L, vitamin solution 0.02ml / L, water balance.

[0035] The composition of Se / W solution per liter is: NaOH 0.5g, NaSeO 3 ·5H 2 O 3mg, Na 2 WO 4 ·2H 2 O 4mg, water balance...

Embodiment 2

[0039] A biological preparation that can mediate the anaerobic reduction and dechlorination of polychlorinated biphenyls. It is made by mixing the following components: 1L of inorganic salt medium, 2mg of anaerobic microorganisms, 2.5ml of sodium lactate, 2mL of vitamin solution, and 6g of rape stalk biochar. .

[0040] In terms of mass percentage, the anaerobic microorganisms consist of the following species: Desulfuromonassp. 45.2%, Desulfitobacterium sp. 25.8% Exiguobacterium sp. 5.3%, Citrobacter sp. (Citrobactersp.) ) 6.5%, Alkaliphilus sp. 4.9%, Pseudomonassp. 2.3%, Dehalobacters p. 3.8%, Bellilinea sp. 3.4%, Dehalogenimonas sp. 2.8%.

[0041] Others are the same as in Example 1.

Embodiment 3

[0043] A biological preparation that can mediate the anaerobic reduction and dechlorination of PCBs, which is made by mixing the following components: 1L inorganic salt medium, 1.5mg anaerobic microorganisms, 2ml sodium lactate, 1.5mL vitamin solution, rape stalk biochar 5g.

[0044] In terms of mass percentages, the anaerobic microorganisms consist of the following species: Desulfuromonassp. 45.5%, Desulfitobacterium sp. 21.6% Exiguobacterium sp. 5.2%, Citrobacter sp. (Citrobactersp.) ) 8.1%, Alkaliphilus sp. 4.7%, Pseudomonassp. 1.8%, Dehalobacters p. 3.5%, Bellilinea sp. 4.1%, Dehalogenimonas sp. 5.5%.

[0045] Others are the same as in Example 1.

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Abstract

The invention discloses a biochar preparation for anaerobic reductive dechlorination of mediated polychlorinated biphenyls. The biochar preparation is prepared from the following components of, by weight, 1 L of an inorganic salt culture medium, 1.5-2.5 Ml of sodium lactate, 1-2 mL of a vitamin solution, 1-2 mg of an anaerobic microorganism, and 4-6 g of rape stem biochar. The biological preparation has the characteristics of being environment-friendly, non-toxic and the like, anaerobic reductive dechlorination of the polychlorinated biphenyls can be effectively mediated, and the biological preparation provides a new way for degrading of the polychlorinated biphenyls.

Description

Technical field [0001] The invention specifically relates to a biochar preparation capable of mediating the anaerobic reduction and dechlorination of polychlorinated biphenyls, and relates to the field of organic matter biodegradation. Background technique [0002] Polychlorinated biphenyls (PCBs) is a general term for a class of chlorinated aromatic compounds in which hydrogen on biphenyl is substituted by chlorine atoms to varying degrees, and contains 209 homologs. Due to its good chemical stability, flame retardancy and insulation, it has been widely used in electrical equipment (such as transformers, transformers, etc.), insulation media, carbon paper, ink, paint, lubricants, plasticizers and other PCBs with high The characteristics of toxicity, environmental persistence, bioaccumulation, and long-distance migration. Due to the very stable structure of PCBs, it is difficult to be degraded in the environment. At the same time, it has low water solubility and high fat solubili...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02C12N1/20C12R1/01C12R1/38
CPCA62D3/02A62D2101/22C12N1/20
Inventor 张宁章春芳张冬冬张志超余新威
Owner ZHEJIANG UNIV
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