Thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof

A technology of carbon monoxide and streptomyces, applied in the direction of application, bacteria, biochemical equipment and methods, etc., can solve the problems of complex composting process of garden waste

Active Publication Date: 2015-10-21
北京大地聚龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The composting process of garden waste is relatively complicated, a

Method used

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  • Thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof
  • Thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof
  • Thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1, Streptomyces thermophilic carbon monoxide strain Dstr3-3 of the present invention

[0066] One, the isolation and purification of the bacterial strain Dstr3-3

[0067] Using Gao's No. 1 medium as the isolation medium, the Dstr3-3 strain was isolated and purified from the garden waste compost of Beijing Agricultural College by the dilution plate separation method. The specific steps are as follows:

[0068] Take litter compost to make a suspension, and under aseptic conditions, use a 10ml sterile pipette to draw 10ml of water sample and place it in the first bottle of 90ml sterile water, rinse the pipette three times, and shake it by hand Break up the granular samples in 10 minutes. Take 0.5ml of bacterial liquid and inoculate in beef extract peptone medium, Gao's No. 1 medium, and guaiacol-PDA medium, inoculate 9 plates each, and place them upside down in an incubator at 30°C, 50°C, or 60°C for 3-15 days , and observe the result. Various cultured microo...

Embodiment 2

[0098] Embodiment 2, effect verification of strain Dstr3-3 decomposing lignin ability

[0099] 1. Preliminary screening of lignin decomposition ability

[0100] Selection medium: Guaiacol medium

[0101] When the actinomycete Dstr3-3 was cultured in the guaiacol medium, it produced a discoloration reaction, and it was preliminarily determined that the bacteria had the ability to degrade lignin ( image 3 ).

[0102] 2. Determination of ligninase type

[0103] On the edge of the colonies of two identical plate culture medium, use a hole puncher to make several small holes with a diameter of about 5 mm, and drop a few drops of H 2 o 2 and the mixed solution of pyrol and 0.1mol / L α-naphthol, under the condition of constant temperature environment, observe the size of the color-changing circle at the edge of the colony.

[0104] By using the punching method, a few drops of H were added to the edge of the colony of Dstr3-3 and the color-changing plug respectively. 2 o 2 and ...

Embodiment 3

[0105] Embodiment 3, bacterial strain Dstr3-3 is used for the effect verification of garden waste composting

[0106] In this experiment, garden waste such as dead branches and leaves in the suburbs of Beijing was used as the main raw material, and different materials such as cow dung were added. At the same time, ventilation, water and different mixed strains were added. According to the orthogonal design, 4 factors and 4 levels of composting experiments were carried out to screen different composting materials. The influence of factors on the composting effect of garden waste, in order to provide a basis for the resource utilization of garden waste in the suburbs of Beijing.

[0107] 1 Materials and methods

[0108] 1.1 Materials

[0109] Composting materials: Garden waste was collected from the fall of 2012 autumn greening tree species on the campus of the tutoring unit Beijing Agricultural College; various animal manure was purchased from the surrounding villages of Beiji...

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Abstract

The invention discloses a thermophilic carbon monoxide streptomycete low-temperature subspecies Dstr3-3 and application thereof, and belongs to the field of microbial ecology. The preservation number of thermophilic carbon monoxide streptomycete strains is CGMCC No.10774. The invention further provides a product which uses the thermophilic carbon monoxide streptomycete strains as active ingredients and is used for degrading lignin and a product, an additive and a microbial inoculum used for garden waste compost. In addition, the invention further provides a compost method for garden waste based on the thermophilic carbon monoxide streptomycete strains. The compost time can be effectively shortened by adopting the thermophilic carbon monoxide streptomycete strains for garden waste compost, compost maturity is promoted substantially, and a compost product large in nutrient content and high in quality is obtained.

Description

technical field [0001] The invention belongs to the field of microbial ecology, and in particular relates to a strain of Streptomyces thermocarboxydus and its application in degrading lignin and garden waste composting. Background technique [0002] Garden waste (garden waste, yard waste) mainly refers to the plant residues produced by the natural fall of garden plants or artificial pruning, mainly including leaves, grass clippings, tree and shrub pruning, etc., also known as garden waste. With the rapid development of ecological cities, the amount of green waste in urban gardens is increasing. For example, the available resources of landscaping waste in Beijing are about 4.3998 million tons. If these wastes are not handled properly, they will not only affect the city's beauty, but also may cause air, water and soil pollution, thereby affecting the health of urban residents and the construction of ecological cities. Composting is a biochemical process that uses microorganis...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C05F15/00C05F17/00C12R1/465
CPCY02W30/40
Inventor 刘悦秋刘克峰王树涛高程达蔡建超
Owner 北京大地聚龙生物科技有限公司
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