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Compound probiotics preparation for biological organic fertilizers and preparation method thereof

A technology of compound probiotics and bio-organic fertilizers, which is applied in urban greening, environmental protection, and agricultural applications, can solve the problem that the microbial flora of soil flora cannot be supplemented, the effective number of viable bacteria of bio-organic fertilizers cannot be reached, and the products do not meet the requirements. Bio-organic fertilizers and other issues, to achieve the effect of being suitable for large-scale production and application, improving crop yield and disease resistance, and improving poor soil

Inactive Publication Date: 2012-06-20
周剑平 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, the decomposing and fermentation period is long, and industrial production is not used
Adding decomposing bacteria agent can effectively shorten the fermentation and decomposing cycle and improve production efficiency, but the products produced do not meet the standards of bio-organic fertilizers
Because the added decomposing bacteria are inconsistent with the soil microbial flora, the soil flora microbial flora cannot be supplemented; in addition, although some decomposing bacteria contain strains in the soil microbial flora, most of them are added before granulation The production process of probiotics will kill these beneficial microbial flora due to high temperature granulation
Therefore, the total number of effective viable bacteria (≥0.2 billion / g) required by the national standard for bio-organic fertilizers cannot be reached.

Method used

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  • Compound probiotics preparation for biological organic fertilizers and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034]Use the mixed inoculation method to activate the good strains, according to Pseudomonas: Streptomyces mutatis mutans: Rhizobium: Brown spherical nitrogen-fixing bacteria: Bacillus subtilis: Bacillus amyloliquefaciens: Bacillus cereus: Bacillus licheniformis: Webster Bacillus: jelly-like Bacillus: Bacillus thuringiensis: Saccharomyces luteus: Streptomyces griseus rust var: Streptomyces luteus=1:1:1:1:1:1:1:1:1:1:1: The ratio of 1:1:1 was mixed and inoculated into 500mL liquid seed medium, cultured on a shaker at 28°C with a speed of 200 rpm, and fermented for 2 days. Then transferred to the 5L secondary seed shake flask, cultured on a shaker at 28°C with a rotation speed of 200 rpm, and fermented for 2 days. Transfer to 50L seed tank again, temperature 28 ℃, rotating speed 200 rpm, ferment for 2 days. The strains in the seed tank were transported to a 500L fermenter at a temperature of 28°C and a rotation speed of 200 rpm, and fermented for 3 days to obtain a liquid comp...

Embodiment 2

[0041] Change the fermentation time and temperature in embodiment 1, namely the fermentation temperature of first-level liquid seed, second-level liquid seed, seed tank and expanded cultivation has 28 ℃ to change to 30 ℃, and the fermentation time has 2 days to prolong to 3 days, simultaneously Molasses concentration increased from 3% to 5%. The obtained liquid composite probiotic agent was mixed evenly with 5 tons of weathered coal, and the stacking time was extended from 3 days to 5 days. Under this condition, solid powder is made, and the effective number of viable bacteria of the solid composite probiotic is 3.8 billion / gram.

[0042] Liquid seed medium formula is identical with embodiment 1.

[0043] The composition and mass content of the 500L fermentation tank culture medium are: molasses 5%, that is 25000g; beef extract 0.3%, that is 1500g; peptone 1%, that is 5000g; sodium chloride 0.5%, that is 2500g; magnesium sulfate 0.05%, that is 250g; Potassium dihydrogen phos...

Embodiment 3

[0045] Change the pH6.8 of the fermentation medium in Example 1 to pH7.4, and the fermentation temperature and fermentation time are the same as in Example 2. That is, in terms of Pseudomonas: Streptomyces violaceum: Rhizobium: Azotobacter sphaeroides: Bacillus subtilis: Bacillus amyloliquefaciens: Bacillus cereus: Bacillus licheniformis: Bacillus weideri: Bacillus jelly-like: Bacillus thuringiensis: Saccharomyces luteus: Streptomyces griseus rust variant: Streptomyces luteus = 1:1:1:1:1:1:1:1:1:1:1:1:1:1:1:1 ratio inoculation, The fermentation temperature of the first-grade liquid seeds, second-grade liquid seeds, seed tanks and expanded cultivation is 30°C, the fermentation time is 3 days, the pH value is 7.4, the concentration of molasses is 3%, and the stacking time is 5 days. Under this condition, solid powder is made, and the effective number of viable bacteria of this solid composite probiotic is 3.5 billion / gram.

[0046] Liquid seed medium formula is identical with e...

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Abstract

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus,Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

Description

technical field [0001] The invention relates to a biological organic fertilizer compound probiotic agent and a preparation method thereof, belonging to the fields of agricultural application, urban greening, environmental protection and the like. Background technique [0002] Livestock and poultry manure, crop stalks, urban domestic waste and garden plant waste are the direct sources of disturbing the world's environment and people's health. And due to the long-term unreasonable use of chemical fertilizers, the proportion of nutrients in some farmland is out of balance, resulting in different degrees of damage to the ecological environment of farmland, soil physical and chemical properties and floristic soil microorganisms, endangering the safety of agricultural products and people's lives to a certain extent. quality. The bio-organic fertilizer not only provides the nutrients needed for plant growth, but also supplements the microorganisms of the soil flora, and can also e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C05F11/08C05F17/00C05F9/04C05G1/00C12R1/38C12R1/465C12R1/41C12R1/065C12R1/125C12R1/07C12R1/085C12R1/10
CPCY02A40/20Y02W30/40
Inventor 周剑平祝英王治业祁宏山季彬宗有祥
Owner 周剑平
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