Fermentation bacteria agent and preparation method and application thereof

Abstract

The invention discloses a fermentation bacteria agent and a preparation method and an application thereof. The preparation method comprises the following steps: (1) mixing unsterilized cabbage stems, orange peels and wheat bran according to the weight ratio of (0.8-1.2):(1.5-1.9):(0.3-0.5), and adding water to wet the mixture, thereby obtaining a culturing substrate; (2) inoculating Aspergillus niger, Trichoderma koningii and wheat koji into the culturing substrate to be stirred evenly; and (3) carrying out a stationary culturing, thereby obtaining the fermentation bacteria agent. The invention further provides the fermentation bacteria agent obtained by utilizing the preparation method and the application thereof in the production of a cellulose compound enzyme system. Compared with the prior art, the preparation method provided by the invention has the advantages that microbes for producing enzyme are tamed by utilizing the natural bacteria strains on the unsterilized carriers, so that the obtained fermentation bacteria agent is stable in microbial ecology and is capable of performing a diastatic fermentation on a cellulose substrate to produce the cellulose compound enzyme system.

Description

technical field [0001] The invention relates to the field of utilization of fiber substrates, in particular to a fermentation agent and its preparation method and application. Background technique [0002] Feed is the material basis for the development of animal husbandry, and a large amount of multi-fiber roughage is an inexhaustible and inexhaustible feed resource in the world. [0003] Cellulosic roughage resources include crop straw, bark, shell, core, waste wood, sawdust, etc. According to statistics, the annual output of crop straw in the world is about 4 billion tons, and our country has about 700-800 million tons per year. These resources have high crude fiber content, low protein, high crude ash, poor palatability, and are not suitable for direct use as feed. low and heavily polluted. [0004] Chinese cabbage is a widely planted vegetable in my country. Its cultivation area and consumption rank first among all kinds of vegetables in my country, and it can be seen ...

AI Technical Summary

Problems solved by technology

However, due to the loss of the coexistence of other bacteria that have been in a long-term synergistic relationship under natural conditions, the bacterial species in the purified single bacterial agent have greatly reduced their enzyme-producing ability, and the cellulose compound enzyme system produced is often incomplete, and has higher requirements for fermentation conditions. and susceptible to contamination

Images