Fermentation bacteria agent and preparation method and application thereof

A technology for fermenting inoculum and culturing substrate, which is applied in the field of fermentation inoculum and its preparation, can solve the problems of incomplete cellulose composite enzyme system, high requirements on fermentation conditions, reduced enzyme production capacity, etc., and achieves stable microecology and long shelf life. , easy to transport and use

Inactive Publication Date: 2013-04-24
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

However, due to the loss of the coexistence of other bacteria that have been in a long-term synergistic relationship under natural conditions, the bacterial species in the purified single bacterial agent ha...

Method used

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  • Fermentation bacteria agent and preparation method and application thereof
  • Fermentation bacteria agent and preparation method and application thereof

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Embodiment Construction

[0036] 1 Substrate Preparation

[0037] Crush cabbage waste and orange peel to 1-2 mm, dry at 60°C for 3 hours, and set aside. Wheat bran was provided by Angiew Felt Brewery.

[0038] 2 Preparation of fermentation agent

[0039] Aspergillus niger ZJU-RYD1 and Trichodermakoningii ZJU-RDY2 are preserved by the Institute of Food Science and Fermentation Engineering, Zhejiang University. The raw wheat koji is provided by Angiewu Felt Brewery.

[0040] Aspergillus niger (Aspergillus niger) ZJU-RYD1 and Trichodermakoningii (Trichodermakoningii) ZJU-RDY2 were respectively inoculated on the slant of PDA medium, and cultured at 28°C for 3-4d for activation.

[0041] The formula of PDA medium is as follows: 200g potato, chopped and boiled for 30min, the filtrate is obtained by filtering with eight layers of gauze; glucose 20g, agar 15g, water 1000mL, pH natural.

[0042] Obtain the spores of activated Aspergillus niger and Trichoderma konii, and prepare them with distilled water at ...

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Abstract

The invention discloses a fermentation bacteria agent and a preparation method and an application thereof. The preparation method comprises the following steps: (1) mixing unsterilized cabbage stems, orange peels and wheat bran according to the weight ratio of (0.8-1.2):(1.5-1.9):(0.3-0.5), and adding water to wet the mixture, thereby obtaining a culturing substrate; (2) inoculating Aspergillus niger, Trichoderma koningii and wheat koji into the culturing substrate to be stirred evenly; and (3) carrying out a stationary culturing, thereby obtaining the fermentation bacteria agent. The invention further provides the fermentation bacteria agent obtained by utilizing the preparation method and the application thereof in the production of a cellulose compound enzyme system. Compared with the prior art, the preparation method provided by the invention has the advantages that microbes for producing enzyme are tamed by utilizing the natural bacteria strains on the unsterilized carriers, so that the obtained fermentation bacteria agent is stable in microbial ecology and is capable of performing a diastatic fermentation on a cellulose substrate to produce the cellulose compound enzyme system.

Description

technical field [0001] The invention relates to the field of utilization of fiber substrates, in particular to a fermentation agent and its preparation method and application. Background technique [0002] Feed is the material basis for the development of animal husbandry, and a large amount of multi-fiber roughage is an inexhaustible and inexhaustible feed resource in the world. [0003] Cellulosic roughage resources include crop straw, bark, shell, core, waste wood, sawdust, etc. According to statistics, the annual output of crop straw in the world is about 4 billion tons, and our country has about 700-800 million tons per year. These resources have high crude fiber content, low protein, high crude ash, poor palatability, and are not suitable for direct use as feed. low and heavily polluted. [0004] Chinese cabbage is a widely planted vegetable in my country. Its cultivation area and consumption rank first among all kinds of vegetables in my country, and it can be seen ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N9/88C12N9/42C12N9/30C12N9/24C12N9/18C12R1/685C12R1/885
Inventor 阮晖杨璐伍星主何李琳李青青徐娟陈功诸文颖杜姗姗刘松柏何国庆
Owner ZHEJIANG UNIV
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