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Method for remedying salmonella-polluted soil by utilizing antagonistic microbe agent

A microbial agent, salmonella technology, applied in the field of microbial remediation, to achieve the effect of protecting soil ecological function, avoiding secondary pollution, and avoiding damage to soil structure and soil organisms

Inactive Publication Date: 2010-01-20
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on microbial remediation of Salmonella-contaminated soil at home and abroad.

Method used

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  • Method for remedying salmonella-polluted soil by utilizing antagonistic microbe agent
  • Method for remedying salmonella-polluted soil by utilizing antagonistic microbe agent
  • Method for remedying salmonella-polluted soil by utilizing antagonistic microbe agent

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Experimental program
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Effect test

Embodiment 1

[0011] 1. Isolation and screening of antagonistic bacterial strains

[0012] 1.1 Medium

[0013] Salmonella identification medium: Magnesium Chloride Malachite Green Enrichment Medium, HE Agar Medium

[0014] Bacterial medium: LB (Luria-Bertani) medium: peptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000ml, pH 7.0, sterilized at 121°C for 20min; add agar powder 1.2-1.5% to the solid medium.

[0015] 1.2 Salmonella strains tested

[0016] Standard strains: Salmonella enteritidis, Salmonella typhimurium.

[0017] Salmonella strains isolated from natural environment: isolated from soil samples contaminated with livestock and poultry manure.

[0018] 1.3 Separation and screening method:

[0019] Isolation of bacterial strains: Weigh 5g fresh soil samples into 50ml sterile water respectively, shake for 45 minutes, then graded dilution (10 -3 -10 -8 ) spread on bacterial LB medium. After the colony grew out, a single colony was selected and inoculated in a 96-well p...

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Abstract

The invention discloses a method for remedying salmonella-polluted soil by utilizing antagonistic microbe agent, comprising the following steps: separating the salmonella-polluted soil by utilizing an aseptic filter paper plate method to obtain antagonistic bacterium suitable for the soil environment and having the function of inhibiting or killing the salmonella; fermenting antagonistic microbe strains through the following processes: inoculum inoculation, first-stage culture, second-stage culture and inoculation and second-stage extensive culture to obtain antagonistic bacteria liquid; spraying the antagonistic bacteria liquid to the salmonella-polluted soil, and then uniformly raking the soil. In the method, the microbial ecology principle is applied, and the function of inhibiting or killing the antagonistic bacterium of the salmonella which is obtained from the soil separated and screened from the soil is utilized to remedy the in-situ microbes for the salmonella-polluted soil, not only the damage of the physical remediation to the soil structure and the soil organism is avoided, but also the secondary pollution caused by the chemical remediation is avoided, therefore, the method is a low-cost and environmentally friendly remediation way.

Description

1. Technical field [0001] The invention belongs to the field of microbial remediation in soil pollution remediation technology, and in particular relates to a microbial ecological remediation method for remediating Salmonella-contaminated soil by using an antagonistic microbial agent in soil microbial pollution. 2. Background technology [0002] Salmonella is an important pathogen that causes intestinal bacterial infection and bacterial food poisoning. Globally, Salmonella poisoning incidents have risen sharply in recent years, many of which are caused by eating Salmonella-contaminated food and drinking Salmonella-contaminated water, so people pay more attention to soil pathogen contamination that causes food and water pollution. Studies have shown that a large number of human, livestock, and poultry manures that have not been properly treated are applied to the soil and domestic and breeding sewage are discharged into the soil environment. These are the main factors that in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12Q1/04C12N1/20C12R1/42
Inventor 尹睿宋德显林先贵
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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