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A method of consolidating loose sand particles using phosphate mineralizing bacteria

A technology of mineralizing bacteria and phosphate, applied in the direction of chemical instruments and methods, applications, soil protection, etc., to achieve the effect of not being easy to secondary pollution, low cost, and small environmental impact

Active Publication Date: 2015-08-19
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current microbial immobilization studies focus on the immobilization of Bacillus pasteuriani

Method used

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  • A method of consolidating loose sand particles using phosphate mineralizing bacteria
  • A method of consolidating loose sand particles using phosphate mineralizing bacteria
  • A method of consolidating loose sand particles using phosphate mineralizing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Weigh 5g of peptone, 3g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 7.0, sterilize and dry, inoculate Bacillus subtilis into the prepared medium, and carry out shaking culture at 30°C , the oscillation frequency is 170r / min, cultivated for 24h; then weigh 4g of disodium phenyl phosphate and put it in the 100mL bacterial liquid prepared above to obtain the bacterial liquid containing disodium phenyl phosphate;

[0041] (2) Configure CaCl 2 The solution concentration is 0.5mol L -1 ;

[0042] (3) Weigh 56.56g of sand with a particle size below 0.15mm and 23.44g of sand with a particle size of 0.15-0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each loading process to The loaded sand is densely packed, and the tight void ratio is 43.1%;

[0043] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution containing disodium phenyl ph...

Embodiment 2

[0047] (1) Weigh 5g of peptone, 3g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 7.0, sterilize and dry, inoculate Bacillus subtilis into the prepared medium, and carry out shaking culture at 30°C , the oscillation frequency was 170r / min, and cultured for 16h; then weighed 5g of disodium phenyl phosphate and put it in the 100mL bacterial liquid prepared above to obtain the bacterial liquid containing disodium phenyl phosphate;

[0048] (2) Configure CaCl 2 The solution concentration is 1mol L -1 ;

[0049] (3) Weigh 63.63g of sand with a particle size below 0.15mm and 26.37g of sand with a particle size of 0.15-0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each loading process to The loaded sand is densely packed, and the tight void ratio is 42.8%;

[0050] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution containing disodium phenyl...

Embodiment 3

[0052] (1) Weigh 5g of peptone, 3g of beef extract and 1000ml of distilled water to prepare a medium, adjust the pH to 8.0, sterilize and dry, inoculate Bacillus subtilis into the prepared medium, and carry out shaking culture at 30°C , the oscillation frequency is 170r / min, and cultured for 24h; then weigh 6g of disodium phenyl phosphate and put it in the 100mL bacterial liquid prepared above to obtain the bacterial liquid containing disodium phenyl phosphate;

[0053] (2) Configure CaCl 2 The solution concentration is 1.5mol L -1 ;

[0054] (3) Weigh 49.49g of sand with a particle size below 0.15mm and 20.51g of sand with a particle size of 0.15-0.3mm, and put them into a 60ml disposable syringe in three times, and tap the outside of the syringe during each filling process to The loaded sand is densely packed, and the tight void ratio is 42.6%;

[0055] (4) Connect the bottom of the syringe with a peristaltic pump, inject the bacterial solution containing disodium phenyl ...

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Abstract

The invention discloses a method utilizing phosphate mineralized bacteria to solidify and loosen loose sand particles. The method comprises the steps that bacillus subtilis is inoculated to beef extract and a peptone culture medium for obtaining bacterial liquid, then disodium phenyl phosphate is added to the prepared bacterial liquid, an anhydrous calcium chloride solution with the concentration of 0.5-1.5mol / L is prepared, 70-90g of sand with two levels of less than 0.15mm and of 0.15-0.30mm is prepared according to the closest Fuller packing method and then loaded into a trial mould with a buffering pad and a sand filter, the bacterial liquid and a chemical solution are respectively poured into the prepared sand in the third step through a peristaltic pump according to a volume ratio of 1:1, the flowing speed of the bacterial liquid is controlled to be 4-6mL / min, the flowing speed of the chemical solution is 10-15mL / min, continuous pouring is carried out for 8-15 days, and the sand with the mould is placed into an oven of 60 DEG C for being conserved for 48h and then taken out and released from the mould. The compressive strength of the sand can reach 1.0MPa.

Description

technical field [0001] The invention relates to a method for strengthening foundation, in particular to a method for using phosphate mineralization bacteria to consolidate loose sand particles. Background technique [0002] At present, the chemical reinforcement materials used to strengthen the foundation are artificial chemical materials such as Portland cement, lime, gypsum, water glass and epoxy resin. These materials, except epoxy resin, all have the impact of polluting the environment and destroying the ecology. [0003] With the advancement of science and technology, more and more people are now paying attention to the field of microbial reinforcement, which is a process of forming inorganic minerals in organisms, in which the metabolism of organisms, cells, and organic substrates are involved. There are two forms, one is that the metabolites of organisms directly form minerals with intracellular and extracellular cations, such as intercellular aragonite of some algae...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): E02D3/12C09K17/00C09K103/00
Inventor 钱春香荣辉王欣
Owner SOUTHEAST UNIV
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