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Microbial preparation for mineralized and consolidated copper ions and use method thereof

A microbial preparation, copper ion technology, applied in chemical instruments and methods, biological water/sewage treatment, water pollutants, etc., can solve problems such as limitations and few microorganisms to repair heavy metal soil, achieve low environmental impact, and ensure non-migration. Sexual, resource-rich effects

Inactive Publication Date: 2011-08-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, so far, most microbial remediation technologies for soil heavy metal pollution are still limited to the laboratory level, and there are few examples of microbial remediation of heavy metal soils.

Method used

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  • Microbial preparation for mineralized and consolidated copper ions and use method thereof
  • Microbial preparation for mineralized and consolidated copper ions and use method thereof
  • Microbial preparation for mineralized and consolidated copper ions and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Prepare 100ml of culture solution, each liter of culture solution contains 5g of peptone and 3g of beef extract, adjust the pH of the culture solution to 7 with NaOH solution, and put it into an autoclave for sterilization. After sterilization, inoculate Bacillus pasteurii (Bacillus pasteurii) into fresh culture medium with an inoculation amount of 2% volume ratio, place it in a shaker at 170rpm, and cultivate it at a constant temperature of 37°C, take it out after 24h, and freeze and centrifuge at 0°C to obtain the strain Cells, refrigerated until use. Get Bacillus pasteurii (Bacillus pasteurii) 0.04g, urea CO(NH 2 ) 2 0.8 g of CuCl in 100 mL 2 solution, CuCl 2 Concentration is 0.2mol / L, blue precipitate appears in the solution after 1h, weighs after filtering and drying, and the data are listed in Table 1, Cu 2+ The mineralization rate is 84.52%.

[0028] Table 1Cu 2+ Mineralization rate

[0029]

[0030] Note: Assume all copper ions are converted to Cu 2 (...

Embodiment 2

[0035] Prepare 100ml of culture solution, each liter of culture solution contains 5g of peptone and 3g of beef extract, adjust the pH of the culture solution to 7 with NaOH solution, and put it into an autoclave for sterilization. After sterilization, inoculate Bacillus pasteurii (Bacillus pasteurii) into fresh culture medium with an inoculum of 2% volume ratio, place it in a shaker at 170rpm, and cultivate it at a constant temperature of 37°C, take it out after 24h, freeze and centrifuge at 0°C, and obtain the strain Cells, refrigerated until use. Get Bacillus pasteurii (Bacillus pasteurii) 0.04g, urea CO(NH 2 ) 2 0.6 g of CuCl in 100 mL 2 solution, CuCl 2 Concentration is 0.2mol / L, blue precipitate appears in the solution after 1h, weighs after filtering and drying, and the data are listed in Table 2, Cu 2+ The mineralization rate is 81.31%.

[0036] Table 2Cu 2+ Mineralization rate

[0037]

[0038] Note: Assume all copper ions are converted to Cu 2 (OH) 2 CO 3...

Embodiment 3

[0040]Prepare 100ml of culture solution, each liter of culture solution contains 5g of peptone and 3g of beef extract, adjust the pH of the culture solution to 7 with NaOH solution, and put it into an autoclave for sterilization. After sterilization, inoculate Bacillus pasteurii (Bacillus pasteurii) into fresh culture medium with an inoculation amount of 2% volume ratio, place it in a shaker at 170rpm, and cultivate it at a constant temperature of 37°C, take it out after 24h, and freeze and centrifuge at 0°C to obtain the strain Cells, refrigerated until use. Get Bacillus pasteurii (Bacillus pasteurii) 0.06g, urea CO(NH 2 ) 2 0.8 g of CuCl in 100 mL 2 solution, CuCl 2 The concentration was 0.2mol / L, blue precipitate appeared in the solution after 1h, weighed after filtering and drying, and the data are listed in Table 3, Cu 2+ The mineralization rate is 88.6%.

[0041] Table 3Cu 2+ Mineralization rate

[0042]

[0043] Note: Assume all copper ions are converted to Cu...

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Abstract

The invention discloses a microbial preparation for mineralized and consolidated copper ions and a use method thereof. The microbial preparation comprises double components of urine and pasteur bacilli. 0.2-0.6g / L of pasteur bacilli and 5-10g / L of urine CO(NH2)2 in proportion are sprayed to a polluted area of the copper ions after being mixed, and are decomposed to produce carbonate ions by adopting the pasteur bacilli and utilizing the enzymatic function of microorganisms under the induction of the urine; Cu2+ in the solution can be effectively mineralized and consolidated into CuCO3 and more stable Cu2(OH)2CO3 within 1h; and the efficient removal rate of the Cu2+ reaches over 90%.

Description

technical field [0001] The invention relates to a microbial preparation for mineralizing and consolidating copper ions, which uses the enzymatic action of microorganisms induced by urea to decompose and produce carbonate ions, which can decompose Cu in the environment within 1 hour. 2+ Effectively consolidates to the mineral state. Background technique [0002] Heavy metal pollution has become a global problem. Whether in soil or water, various heavy metals have entered the bottom of the human food chain and entered the human body along with the food chain, seriously threatening human health. In recent years, heavy metals Pollution control has become an important direction for the full support of governments and the research institutions of various countries. [0003] There are many traditional physical and chemical methods to deal with heavy metals, but there are disadvantages such as large investment, high energy consumption, difficult operation, and easy to produce secon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C02F3/34C02F101/20
Inventor 钱春香陆兆文王瑞兴
Owner SOUTHEAST UNIV
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