Method for analyzing microbial community structure of fermented grain

A technology for microbial communities and fermented grains, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as incompatibility and difficult cultivation, and achieve the effect of saving time and simple operation.
CN103031384AInactive Publication Date: 2013-04-10LUZHOU PINCHUANG TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
LUZHOU PINCHUANG TECH CO LTD
Publication Date
2013-04-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for analyzing a microbial community structure of a fermented grain, belongs to the technical field of microbial ecology and aims to provide the method for analyzing the microbial community structure of the fermented grain. The technical scheme of the invention is as follows: the method for analyzing the microbial community structure of the fermented grain comprises extracting genomic DNA (Deoxyribose Nucleic Acid), expanding PCR (Polymerase Chain Reaction) of 16S rDNA, carrying out electrophoresis on DGGE (Denaturing Gradient Gel Electrophoresis), recycling a single strip of the 16S rDNA which is subjected to electrophoresis, and analyzing the recycled strip, wherein the step of analyzing the recycled strip comprises the following steps: establishing a clone bank by using the recycled 16S rDNA which is subjected to electrophoresis, carrying out enzyme segmentation, testing the sequence and carrying out comparison and analysis on GenBank so as to obtain the community structure information of the microorganism in the fermented grain. The method provided by the invention is widely applied to microbial community structure analysis and change regulation research in the white wine brewing process.
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Description

Technical field

[0001] The invention relates to a method for analyzing the microbial community structure of fermented wine, belonging to the technical field of microbial ecology. Background technique

[0002] Denaturing gradient gel electrophoresis based on polymerase chain reaction (denaturing gradient gel electrophoresis) has been widely used in recent years as a molecular fingerprint analysis technique for microbial genetic diversity analysis in different environments. In 1993, Muyer et al. first applied this technology to the study of microbial ecology, and subsequently used it extensively in the study of genetic diversity and population differences in natural microbial communities. According to the melting characteristics of DNA, the denaturant concentration required for the denaturation of DNA double helices with different base composition is different. When mixed double-stranded DNA is electrophoresed on a polyacrylamide gel with a linear gradient of denaturant concentrati...

Claims

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