Method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology

A technology of stable isotope and denitrifying bacteria is applied in the field of active short-range denitrifying bacteria and triclosan degrading bacteria, which can solve the problems of unclear relationship, and achieve the effect of improving the identification rate and the distribution ratio.

Active Publication Date: 2021-04-06
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the active short-range denitrifiers and triclosan-degrading bacteria in the granular sludge short-range denitrification system have not been revealed, and the relationship between the active short-range denitrifiers and triclosan-degrading bacteria is not clear. A Bacteria Can Remove Triclosan and Achieve Short-cut Denitrification Simultaneously

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  • Method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology
  • Method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology
  • Method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology

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Embodiment 1

[0033] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, specifically comprising the following steps:

[0034] (1) Start-up of a short-cut denitrification system based on granular sludge and the biological removal of triclosan in this system. Set the concentration of sodium propionate and nitrate nitrogen in the influent to 315mg / L and 105mg / L respectively, inoculate aerobic granular sludge with a sludge concentration of 6000mg / L, and start a short-range denitrification sequence batch biological system with an effective volume of 3L. The reactor achieved a stable nitrous accumulation rate after 65 days of operation, and then added 3mg / L triclosan to the system for a total of 130 days of operation. ) to carry out the mass conservation experiment of triclosan to explore the removal of triclosan in the short-range denitrification system. The results of the mass cons...

Embodiment 2

[0046] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as in Example 1, the difference is: step (1) and (2) in Sodium acetate was changed to the corresponding sodium acetate.

Embodiment 3

[0048] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as in Example 1, the difference is: in steps (1) and (2) Sodium propionate was changed to the corresponding methanol.

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Abstract

The invention discloses a method for simultaneously identifying short-cut denitrifying bacteria and triclosan degrading bacteria based on a DNA stable isotope nucleic acid probe technology, and belongs to the technical field of sewage biological treatment technologies and microbial ecology. The method comprises the steps of (1) starting a short-cut denitrification system based on granular sludge and biologically removing triclosan in the system; (2) carrying out 13C-propionic acid sodium and 13C-triclosan isotope microcosm experiments; (3) separating heavy-layer DNA by adopting an ultra-high-speed centrifugation method based on a cesium chloride density gradient solution; and (4) carrying out DNA marking degree evaluation and flora structure analysis. According to the method for simultaneously identifying active short-cut denitrifying bacteria and triclosan degrading bacteria in the short-cut denitrification granular sludge system based on the DNA stable isotope nucleic acid probe technology, a microorganism capable of simultaneously realizing short-cut denitrification and triclosan removal can be explored and disclosed, and a theoretical support is provided for an actual process of removing triclosan by short-cut denitrification in a sewage treatment plant.

Description

technical field [0001] The invention belongs to the technical fields of sewage biological treatment technology and microbial ecology, and specifically relates to a method for simultaneously identifying active short-range denitrifying bacteria and triclosan-degrading bacteria in a short-range denitrification granular sludge system based on DNA stable isotope nucleic acid probe technology. Background technique [0002] Anammox is considered as a novel autotrophic and cost-effective wastewater biological treatment technology, and nitrite, as one of the substrates of anammox, is used as an electron acceptor to oxidize ammonia nitrogen to nitrogen gas. So far, the common ways to obtain nitrite are short-range nitrification and short-range denitrification. Short-range nitrification, ammonia oxidizing bacteria oxidize ammonia nitrogen to nitrite nitrogen in an aerobic environment. However, in the actual sewage treatment system, the activity of nitrate oxidizing bacteria is higher t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6851C12Q1/6869C12Q1/689C12Q1/04C12R1/01
CPCC12Q1/6851C12Q1/6869C12Q1/689C12Q2523/32C12Q2531/113C12Q2535/122Y02W10/10
Inventor 高景峰戴慧卉王知其赵轶凡崔影超李定昌
Owner BEIJING UNIV OF TECH
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