A method for simultaneous identification of short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology

A stable isotope and nucleic acid probe technology, applied in the field of active short-range denitrifying bacteria and triclosan-degrading bacteria, can solve problems such as unclear relationships, and achieve the effect of improving identification rate and distribution ratio

Active Publication Date: 2022-08-09
BEIJING UNIV OF TECH
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Problems solved by technology

However, the active short-range denitrifiers and triclosan-degrading bacteria in the granular sludge short-range denitrification system have not been revealed, and the relationship between the active short-range denitrifiers and triclosan-degrading bacteria is not clear. A Bacteria Can Remove Triclosan and Achieve Short-cut Denitrification Simultaneously

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  • A method for simultaneous identification of short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology
  • A method for simultaneous identification of short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology
  • A method for simultaneous identification of short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology

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Embodiment 1

[0033] A method for simultaneously identifying short-range denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology, specifically comprising the following steps:

[0034] (1) Start-up of a short-range denitrification system based on granular sludge and biological removal of triclosan in the system. Set the influent sodium propionate and nitrate nitrogen concentrations to 315mg / L and 105mg / L, respectively, inoculate aerobic granular sludge, the sludge concentration is 6000mg / L, and start a short-range denitrification sequencing batch biological with an effective volume of 3L. The reactor achieved a stable nitrous accumulation rate after 65 days of operation, and then added 3 mg / L triclosan to the system for a total of 130 days of operation. ) Take the mud to carry out the triclosan mass conservation experiment to explore the removal of triclosan in the short-range denitrification system. The quality conservation results of...

Embodiment 2

[0046] A method for simultaneously identifying short-range denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as those in Example 1, the difference is: C in steps (1) and (2) Sodium was changed to the corresponding sodium acetate.

Embodiment 3

[0048] A method for simultaneously identifying short-range denitrifying bacteria and triclosan-degrading bacteria based on DNA stable isotope nucleic acid probe technology, the specific steps are basically the same as those in Example 1, the difference is: in steps (1) and (2) Sodium propionate was changed to the corresponding methanol.

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Abstract

A method for simultaneously identifying short-range denitrifying bacteria and triclosan degrading bacteria based on DNA stable isotope nucleic acid probe technology belongs to the technical fields of sewage biological treatment technology and microbial ecology. The method includes the following steps: (1) startup of a short-range denitrification system based on granular sludge and biological removal of triclosan in the system; (2) carrying out 13 C-Sodium Propionate and 13 C-triclosan isotope microcosm experiment; (3) separation of heavy-layer DNA by ultracentrifugation based on cesium chloride density gradient solution; (4) DNA labeling degree evaluation and bacterial community structure analysis. The present invention is based on the DNA stable isotope nucleic acid probe technology to simultaneously identify the active short-range denitrification bacteria and triclosan degrading bacteria in the short-range denitrification granular sludge system, and can explore and reveal a method that can simultaneously realize short-range denitrification and triclosan. The removed microorganisms provide theoretical support for the short-range denitrification removal process of triclosan in actual sewage treatment plants.

Description

technical field [0001] The invention belongs to the technical fields of sewage biological treatment technology and microbial ecology, in particular to a method for simultaneously identifying active short-range denitrifying bacteria and triclosan-degrading bacteria in a short-range denitrifying granular sludge system based on DNA stable isotope nucleic acid probe technology. Background technique [0002] Anammox is considered as a novel autotrophic and cost-effective biological wastewater treatment technology, and nitrite, one of the substrates of anammox, is used as an electron acceptor to oxidize ammonia nitrogen to nitrogen. So far, the common ways to obtain nitrite are short-range nitrification and short-range denitrification. In short-range nitrification, ammonia oxidizing bacteria oxidize ammonia nitrogen to nitrite nitrogen in an aerobic environment. However, in the actual sewage treatment system, the activity of nitrate oxidizing bacteria is higher than that of ammoni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6851C12Q1/6869C12Q1/689C12Q1/04C12R1/01
CPCC12Q1/6851C12Q1/6869C12Q1/689C12Q2523/32C12Q2531/113C12Q2535/122Y02W10/10
Inventor 高景峰戴慧卉王知其赵轶凡崔影超李定昌
Owner BEIJING UNIV OF TECH
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