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Ocean-sourced Microbacterium keratanolyticum MCDA02, method for producing enzyme from ocean-sourced Microbacterium keratanolyticum MCDA02, and product

A technology of keratin and microbacteria, applied in the field of microorganisms, can solve the problems of low enzyme production activity, few reports, poor deacetylation effect and the like

Active Publication Date: 2017-09-01
JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Currently reported chitin deacetylases have problems such as low enzyme activity and poor deacetylation effect
Especially for our country, the research on chitin deacetylase started relatively late, and there are few related reports.

Method used

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  • Ocean-sourced Microbacterium keratanolyticum MCDA02, method for producing enzyme from ocean-sourced Microbacterium keratanolyticum MCDA02, and product
  • Ocean-sourced Microbacterium keratanolyticum MCDA02, method for producing enzyme from ocean-sourced Microbacterium keratanolyticum MCDA02, and product
  • Ocean-sourced Microbacterium keratanolyticum MCDA02, method for producing enzyme from ocean-sourced Microbacterium keratanolyticum MCDA02, and product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1, a marine microbacterium keratanolyticum MCDA02 (Microbacterium keratanolyticum) CGMCC NO.13539. The bacterial strain has the following characteristics: the bacterial strain is Gram-negative short bacilli without spores. After the strain was cultured on 2216E solid culture for 48 hours: yellow, translucent, smooth and moist surface, round shape, neat edges, slightly protruding center, easy to pick. A yellow discolouration can be produced on selection media containing colloidal chitin and p-nitro-N-acetanilide. The strain's starch hydrolysis, indole test, hydrogen sulfide production, and arginine decarboxylase tests were all positive, and the citrate utilization, catalase, gelatin liquefaction, VP test, methyl red, glucose fermentation, and nitrate reduction tests were all positive. Negative. The strain grows slowly below 5°C and above 45°C, and the optimum growth temperature is 30°C. The suitable pH range for growth is 6.0-10.0, and the optimal growth pH is...

Embodiment 2

[0082] Example 2, a method for producing chitin deacetylase by fermentation of Microbacterium keratinolyticum MCDA02 as described in Example 1, the steps are as follows: Inoculate the bacterial strain MCDA02 preserved on the 2216E slant into the seed medium, fill the solution Inoculate the seed liquid with 1% inoculation amount in the enzyme-producing medium, and incubate at 20% liquid volume, 180 r / min, and 30°C for 48 hours , centrifuged at 10000r / min for 10min, and the supernatant was obtained as crude chitin deacetylase enzyme. The seed medium is: yeast powder 0.1%, peptone 0.5%, NaCl 1%, prepared from old sea water, pH 7.0; fermentation medium: corn steep liquor 0.5%, soluble starch 0.3%, NH 4 SO 40.3%, KH 2 PO 4 0.15%, MgSO 4 0.05%, powdered chitin 1%, prepared with aged sea water, pH 8.0.

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Abstract

The invention discloses ocean-sourced Microbacterium keratanolyticum MCDA02 with the collection number of CGMCC NO.13539. The strain is Gram-negative spore-free brevibacterium, and after being cultured for 48 h on a 2216E solid culture medium, the strain is yellow, translucent, smooth and moist in surface, circular, neat in edge, slightly protruding in center and easy to pick up. The strain grows slowly at 5 DEG C and 45 DEG C, and the optimal growth temperature is 30 DEG C; the growth pH range is 6.0-9.0, and the optimal growth pH is 8.0; and when the NaCl concentration is 0-10%, the strain can grow, and the optimal growth NaCl concentration is 3%. The invention further discloses a method for producing chitin deacetylase by using the strain MCDA02 and a product. For the chitin deacetylase produced by the Microbacterium keratanolyticum MCDA02 strain provided by the invention, the optimal pH is 8.0 and the optimal temperature is 30 DEG C; and at a low temperature, the chitin deacetylase has relatively high activity, so that energy can be saved and the cost can be reduced in industrial application.

Description

technical field [0001] The present invention relates to a microorganism, especially a kind of microbacterium keratanolyticum MCDA02 (Microbacterium keratanolyticum) isolated from the sea mud of the Haizhou Bay of the Yellow Sea in China; CGMCC, General Microbiology Center of the Management Committee, has a preservation number of CGMCC NO.13539; the present invention also relates to a method and product for producing chitin deacetylase by the strain. Background technique [0002] Chitin, also known as chitin or chitin, is a structural polysaccharide formed by the polymerization of N-acetylglucosamine through β-1,4-glucosidic bonds. Chitin widely exists in the exoskeleton and epidermis of invertebrates and the cell walls of fungi and algae, and is the second most natural organic compound in the world after cellulose. Chitin is insoluble in liquids such as water, alcohol, weak acid and weak base, so it is not easy to be widely developed and utilized. Chitosan is the N-deacety...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/80C12R1/01
CPCC12N9/80C12Y305/01041C12N1/205C12R2001/01
Inventor 房耀维王淑军刘姝吕明生焦豫良来蒋丽胡晟源顾张慧
Owner JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG
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