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Inoculant, feed or additive and removal method for mycotoxins

A technology of mycotoxins and additives, applied in the field of microorganisms, can solve the problems of decreased removal efficiency, less research, and difficulty in achieving efficient removal

Inactive Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, in terms of biological removal, it is difficult to remove multiple toxins efficiently at the same time. When using a compound bacterial agent, the removal efficiency of one toxin is often improved, while the removal efficiency of other toxins is lower. Decreased
When it comes to fumonisins (FUM), ochratoxins, and T-2 toxins, the three toxins are relatively less well-studied due to their much lower levels relative to deoxynivalenol, aflatoxins, and zearalenone toxins. less, it is more difficult to achieve efficient removal of them at the same time

Method used

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  • Inoculant, feed or additive and removal method for mycotoxins
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  • Inoculant, feed or additive and removal method for mycotoxins

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preparation example Construction

[0038] The present invention also includes the preparation method of the bacterial agent, wherein, the method comprises respectively inoculating at least two of the above-mentioned molds, yeasts and bacteria in the corresponding medium for cultivation, and then inoculating the obtained The culture is preferably compounded according to the above proportions to obtain the microbial agent of the present invention.

[0039] In the present invention, the method of inoculating the mold, yeast and bacteria in the culture medium can be changed in a wide range, for example, the method can include: respectively cultivating the mold, yeast and bacteria in separate culture systems Bacteria; and the respectively cultured mold, yeast and bacteria are mixed in proportion to obtain the microbial inoculant.

[0040] In the second aspect, the present invention also provides the application of the above bacterial agent in removing mycotoxins.

[0041] According to the present invention, the myc...

preparation example

[0067] Inoculate mold spores and yeast in PDA medium with 1% inoculum respectively, and cultivate them at 37°C for a period of time to make the concentration of mold spores 10 9 CFU / ml, the concentration of yeast is 10 9 CFU / ml. Bacteria were inoculated in LB medium with an inoculum of 1%, cultured at 37°C, and the culture time was such that the concentration of bacteria was 10 9 CFU / ml.

[0068] The bacterium liquid after cultivating is carried out the preparation of composite bacterial agent according to the proportioning of each embodiment in the following table 1, and in the final bacterial agent, the total number of viable bacteria contained in each gram of the bacterial agent is 10 9 CFU.

[0069] Table 1

[0070]

[0071]

Embodiment 1-7

[0073] Used to illustrate the removal of bacterial agents provided by the invention to mycotoxins

[0074] Carry out the preparation of bacterial agent according to the proportioning of each embodiment in Table 1, then respectively add 0.5ppm ochratoxin A, 0.5ppm fumonisin and 0.5ppm T2 toxin in the prepared bacterial agent, after adding, in 37 ℃, After reacting for 2 hours under the condition of pH value 7, 20 μL of the treated sample was taken into high performance liquid chromatography to detect the residues of each toxin, and the removal rate was calculated. The results are shown in Table 2.

[0075] Wherein, toxin removal rate=(toxin initial concentration-toxin concentration after treatment) / toxin initial concentration×100%.

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Abstract

Relating to the field of microorganisms, the invention discloses an inoculant, a feed or additive and a removal method for mycotoxins. The invention specifically discloses an inoculant, which includesat least two of mould, yeast and bacteria, wherein the bacteria can be at least one of brevibacterium, acinetobacter, rhodococcus and pseudomonas. Specifically, the inoculant can remove mycotoxins. The invention also discloses application of the inoculant in removal of mycotoxins, an additive containing the inoculant and a removal method for mycotoxins. According to the technical scheme, combineduse of at least two of the yeast, mould and bacteria can achieve simultaneous removal of ochratoxin A, fumonisins and T-2 toxin, and the removal efficiency of ochratoxin A and fumonisins is the highest.

Description

technical field [0001] The invention relates to the field of microbes, in particular to a microbial agent and a feed or additive containing the agent, as well as the application of the agent in removing mycotoxins and a method for removing mycotoxins. Background technique [0002] Mycotoxins are a class of secondary metabolites produced by toxin-producing fungi during the damage process, mainly including aflatoxin, deoxynivalenol (also known as vomitoxin, DON), zearalenone (ZEN) and Fumonisins (FUM), ochratoxins and T-2 toxins, etc. [0003] In recent years, due to the frequent occurrence of extreme climates in my country, the head blight that mainly harms wheat and corn has become a pandemic, and the pollution of fumonisins (FUM), ochratoxin and T-2 produced by fungi has increased. Excessive wheat and corn are on the rise. Research on the safe use of grains with excessive toxins can not only protect the interests of farmers in our country, but also prevent them from flowin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14A23K10/18A23L5/20C12R1/645C12R1/84C12R1/01C12R1/40C12R1/685C12R1/10C12R1/125C12R1/23C12R1/245C12R1/225C12R1/25C12R1/72C12R1/46C12R1/69C12R1/07
CPCC12N1/14C12N1/16C12N1/20
Inventor 苏会波林海龙熊强唐堂谭剑黄锦张子剑李文钊李凡陈博朱镜羲刘劲松杨勇范洪岩刘辉
Owner COFCO NUTRITION & HEALTH RES INST
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