Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions and screening and domestication method

A low-temperature domestication, water source water technology, applied in microorganism-based methods, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of secondary environmental pollution, accumulation of nitrite and nitrate, unsuitable treatment, etc.

Inactive Publication Date: 2009-11-04
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0004] The purpose of the present invention is to solve the phenomenon of nitrite and nitrate accumulation in the effluent and the production of N 2 O Causes secondary pollution to the environment and is not suitable for the treatmen

Method used

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  • Bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic conditions and screening and domestication method

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specific Embodiment approach 1

[0011] Specific embodiment 1: In this embodiment, the bacterium SRA13 that can remove ammonia nitrogen in slightly polluted water source water under low temperature and aerobic conditions is Brevibacterium mcbrellneri, which belongs to the genus mcbrellneri, and has been preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee , the preservation number is CGMCC No.2890, and the preservation date is January 19, 2009; the bacteria that remove ammonia nitrogen in slightly polluted water source water under low temperature and aerobic conditions are Gram-negative aerobic bacteria, which are short rod-shaped and 0.4 in length μm~0.9μm, 0.7μm~1.0μm in width, often exist in pairs or clusters, without spores and flagella, and twitching; form yellow colonies on beef extract peptone medium, the colonies are round, and the surface of the colonies is raised and smooth .

[0012] In this embodiment, the bacteria that can remove ammonia ni...

specific Embodiment approach 2

[0013] Specific embodiment two: the difference between this embodiment and specific embodiment two is that the bacteria that can remove ammonia nitrogen in slightly polluted water source water under low temperature and aerobic conditions are positive for catalase and negative for oxidase; the methyl red test is negative and does not produce acetyl Methyl methanol; does not generate H 2 S, does not hydrolyze starch, does not liquefy gelatin; produces indole; the growth pH is 6-8, and the growth temperature is 2-30°C. Others are the same as in the second embodiment.

specific Embodiment approach 3

[0014] Specific implementation method three: this embodiment can remove the screening and domestication method of ammonia nitrogen bacteria in slightly polluted water source water under low temperature and aerobic conditions. After 48 hours of enrichment and culture under aerobic conditions for 1 min, the enrichment solution was obtained. The enrichment solution was serially diluted and spread on LB solid medium, separated and cultured at 28-32°C for 48 hours, and then singles with different characteristics were picked. Colonies were inoculated on LB solid medium and purified for 24 hours; 2. Inoculated the purified strains in LB liquid medium and cultured at 28-32°C for 24 hours to obtain bacterial liquid; 3. Take 1ml of bacteria Inoculate the liquid into the screening medium, and cultivate it under the condition of 28-32°C for 48 hours; 4. Take 1ml of the bacterial liquid of the surviving strains and transfer it to the fresh screening medium, and cultivate it under the condit...

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Abstract

The invention relates to bacteria capable of eliminating ammonian in micro-polluted source water under microthermal and aerobic condition and a screening and domestication method, relating to bacteria and screening and domestication method and solving the problem that the existing bacteria are not suitable for the treatment of the micro-polluted source water and low temperature treatment. The bacterium SRA 13 of the invention is Brevibacterium mcbrellneri, belongs to brevibacterium and is collected in the CGMCC, the collection number is CGMCC No. 2890, and the collection date is 19th, January 2009. The screening method includes the steps as follows: first, separation and purification; second, bacteria solution culture; third, the screening and the culture of the bacteria solution of survival strains; fifth, the progressive low-temperature domestication of the survival strains after the repetition of the step fourth; and sixth, the acquisition of bacteria solution of the survival strains after the low-temperature domestication, and secondary screening. The bacteria of the invention can treat the micro-polluted source water under the condition of low temperature of 2 to 10 DEG C.

Description

technical field [0001] The invention relates to a bacterium and a method for screening and acclimating it. Background technique [0002] Ammonia nitrogen (NH 3 -N) as ionic ammonia (NH 4 + ) and non-ionic ammonia (NH 3 ) exist in two forms in water. Ammonia chlorinated in drinking water exceeding 0.2 mg / L will cause smell and taste problems, and will also reduce the disinfection effect. Ammonia in the water pipe will be nitrous bacteria to generate nitrite, which will cause harm to human health. [0003] With the deepening of research on heterotrophic nitrification-aerobic denitrification, a large number of heterotrophic nitrifying bacteria have been isolated, such as Peseudomonas spp, Alcaligenes faecalis, Thiosphaera pantotropha, Thauera mechernichensis, etc. However, during the treatment of the above strains, the accumulation of nitrite and nitrate in the effluent will occur, which is harmful to the human body and will produce N 2 O, causing secondary pollution to t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 李伟光张多英郜玉楠王广智解丰波
Owner HARBIN INST OF TECH
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