Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of acidophilic heterotrophic bacteria DX-X and use thereof

A technology of heterotrophic acidophilus, DX-X, which is applied in heterotrophic acidophilus and in the application field of improving biometallurgical leaching rate, and can solve environmental problems and other problems

Inactive Publication Date: 2009-07-15
李红玉 +1
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, conventional metal mineral smelting methods have brought serious environmental problems to the environment

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of acidophilic heterotrophic bacteria DX-X and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Characteristics of Acidophilus Heterotroph DX-X Strains

[0022] 1. Inoculate DX-X in BSYG liquid medium with constant temperature shaking and culture for a suitable time, then stain and microscopically check, and perform Gram staining. Staining result: the bacterium is short rod-shaped, G - Sex, movement, solitary.

[0023] 2. Temperature adaptation of heterotrophic acidophilus strain DX-X

[0024] Inoculate 1ml of the same amount of strains into 50ml BSYG liquid medium, place them in 20°C, 25°C, 30°C, 35°C, 40°C, 45°C constant temperature shakers, shake at 120rpm for 48h, and observe their growth situation.

[0025] temperature(℃) 20 25 30 35 40 45 0D 600 0.041 0.301 0.512 0.454 0.247 0.065

[0026] It can be seen from the above table that in the temperature range of 25-40°C, DX-X bacteria can grow well, and the optimum temperature of DX-X bacteria is 30-35°C.

[0027] 3. Osmotic pressure adaptation of heterotrophic acidoph...

Embodiment 2

[0072] The promoting effect of embodiment 2 acidophilus heterotrophic bacteria DX-X strain on the growth of Thiobacillus ferrooxidans:

[0073] Configuration FeTSBo (1.26g (NH 4 ) 2 SO 4 , 0.5gMgSO 4· 7H 2 O, 0.25g peptone, 6.95g FeSO 4 .7H 2O, 12g agar, 1000ml distilled water, pH3.0) double layer solid medium. Spread 50ul of DX-3 bacteria solution on the bottom layer of the FeTSBo double-layer medium, and coat 20ul of Thiobacillus ferrooxidans bacteria solution on the upper layer of the medium, culture it upside down at 30°C, and observe the growth of bacteria after one week.

[0074] blank Medium with DX-3 added Growth of Thiobacillus ferrooxidans - +

[0075] "+" indicates growth and "-" indicates no growth.

Embodiment 3

[0076] The promoting action of embodiment 3 acidophilus heterotrophs DX-X bacterial strain to the leaching rate of Thiobacillus ferrooxidans nickel ore:

[0077] The ore collected from Jinchuan Nickel Mine was processed into particles with a particle size of 165 μm, acidified with 10% sulfuric acid solution, rinsed with distilled water and dried. The nickel content of the ore is: 0.29%.

[0078] Configure 9K medium (3.0g (NH 4 ) 2 SO 4 , 0.1g KCl, 0.5g K 2 HPO 4 , 0.5g MgSO 4· 7H 2 O, 0.01gCa(NO 3 ) 2 , 44.7g FeSO 4· 7H 2 O, distilled water 1000ml, pH2.5) and 9KYE medium (3.0g (NH 4 ) 2 SO 4 , 0.1g KCl, 0.5g K 2 HPO 4 , 0.5g MgSO 4· 7H 2 O, 0.01g Ca(NO 3 ) 2 , 44.7g FeSO 4· 7H 2 O, 1g glucose, 0.1g yeast extract, 1000ml distilled water, pH2.5). Add 3 g of acidified mineral powder to the two media respectively.

[0079] Inoculation: Inoculate 10% Thiobacillus ferrooxidans in 9K medium, inoculate Thiobacillus ferrooxidans and DX-3 at the same time in 9KYE ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an Acidiphilium spp.DX-X and uses thereof, the strain can be cultured in a BSYG liquid culture medium and solid culture medium, and can be grown advantageously within a temperature range from 25 to 40 DEG C and within a pH range from 2.5 to 4.5. The strain is Gram-negative bacterium which is in Brevibacterium, capable of moving and solitary, belongs toacidophilic microorganism, and can be grown on condition of 3% of salt concentration. The strain can not utilize sulfur powders and sodium thiosulfate as well as can not regard Fe as an energy source. Glucose, mannitol and glycerol can be utilized as organic energy sources, oxidase is negative while contact enzyme is positive. The bacterium promotes the growth of Thiobacillus ferrooxidans on the solid culture medium, and has, when implementing a mixed leaching on Jinchuan nickel mine with the Thiobacillus ferrooxidans, a leaching rate superior to that of the Thiobacillus ferrooxidans at the time of leaching individually, therefore, the bacterium possesses enormous potential in case of being applied to bio-hydrometallurgy industry.

Description

technical field [0001] The invention relates to an acidiphilium heterotroph DX-X and its application, mainly relates to an acidiphilium heterotroph (Acidiphilium spp. DX-X) and its application in improving the leaching rate of biometallurgy, and belongs to the category of biometallurgy technology. technical background [0002] Due to people's increasing demand for metal mineral raw materials, the earth's metal mineral resources are increasingly scarce. At the same time, conventional metal mineral smelting methods have brought serious environmental problems to the environment. It forces people to start to adopt the microbial hydrometallurgy technology with low cost, simple process, wide range of functions and little environmental pollution. Today, the recovery of precious metals from low-grade raw materials (such as waste ores) through the use of microbial hydrometallurgy has reached an unprecedented scale. [0003] In 1947, American Colmer and Hinkle isolated and identifie...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C22B3/18B01D53/84B01D53/48C12R1/01
Inventor 李红玉张晓雪鲍婧婷
Owner 李红玉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com