High-yield production method of GABA (gamma amino butyric acid) through mixed fermentation of brevibacterium tianjinese and lactobacillus plantarum

A technology of Lactobacillus plantarum and Brevibacterium tianjini, applied in the fields of fermentation engineering and enzyme engineering, can solve problems such as lack of γ-aminobutyric acid synthesis pathway, and achieve the effects of improving production efficiency, reducing production cost and improving safety
CN103243128AActive Publication Date: 2013-08-14JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2013-08-14

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Abstract

The invention discloses a high-yield production method of GABA (gamma amino butyric acid) through mixed fermentation of brevibacterium tianjinese and lactobacillus plantarum, and belongs to the fields of fermentation engineering and enzyme engineering. According to the invention, glucose is transformed into glutamic acid by using the brevibacterium tianjinese SW07-1, and then glutamic acid of fermentation liquor obtained in the last step is transformed into GABA by using the lactobacillus plantarum GB01-21. Before transformation, the pH value of glutamic acid fermentation liquor is adjusted to be the optimum pH value of glutamic acid decarboxylase, so that a transformation system is built, and in the step 2, after the fermentation liquor is filtrated or centrifuged, the lactobacillus plantarum GB01-21 is obtained, and then the lactobacillus plantarum GB01-21 is added into the transformation system to carry out whole-cell transformation. When original glutamic acid of the fermentation liquor is completely transformed, GABA in the transformation liquor is 59.2 g / L, and the molar transformation rate is 93.6%. After an exogenous glutamic acid is continued to be added and then transformed, the GABA content is 96.5 g / L, and the molar transformation rate is 91.8%.
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Description

technical field

[0001] The invention discloses a method for high-yield GABA mixed fermentation by using safe and efficient Brevibacterium tianjin and Lactobacillus plantarum, belonging to the fields of fermentation engineering and enzyme engineering. technical background

[0002] γ-Aminobutyric acid (GABA) is a non-protein amino acid naturally present in some organisms. It is an important inhibitory neurotransmitter in the central nervous system of mammals and has a wide range of applications in food, feed, medicine and other fields. γ-aminobutyric acid has physiological functions such as anti-anxiety, lowering blood pressure, calming the nerves, enhancing memory, regulating hormone secretion, promoting reproduction, diuresis, and analgesia.

[0003] The preparation of γ-aminobutyric acid is mainly through chemical synthesis and biological methods. The biological method includes plant enrichment method and microbial fermentation method. The chemical method is mainly obtain...

Claims

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